Sentences with phrase «used human cell line»

Now Carette and co-workers have used a similar approach and used a human cell line, in which nearly all human chromosomes are present in a single copy.

Since their isolation in 1951, HeLa cells have been the most widely used human cell line in research.

HeLa cells are the most widely used human cell lines in existence today.

Not exact matches

In August of last year, President Bush approved the use of federal funds to support research on a limited number of existing human embryonic stem cell lines.

Before we established in - house human taste cell (HTC) technologies, it was not possible to use human tongue - derived cells as a model, due to the lack of homogenous, proliferating cell lines with defined properties, which is a prerequisite to establish comprehensive research and screening programs.

As well as allowing the use of stem cells grown from established cell lines, the technology could enable the creation of improved human tissue models for drug testing and potentially even purpose - built replacement organs.

Ongoing attempts to create human stem cell lines using SCNT have yet to achieve success.

Using both fruit fly and human lung cancer cell lines, researchers targeted two of the most common genetic mutations associated with NSCLC — Ras and PTEN (P13K).

To see whether cancer stem cell renewal involves a chain of events similar to that used by embryonic stem cells, and whether the process was affected by oxygen levels, Semenza and graduate student Chuanzhao Zhang focused their studies on two human breast cancer cell lines that responded to low oxygen by ramping up production of the protein ALKBH5, which removes methyl groups from mRNAs.

The laboratory process, described in the journal Scientific Reports, entails genetically modifying a line of human embryonic stem cells to become fluorescent upon their differentiation to retinal ganglion cells, and then using that cell line for development of new differentiation methods and characterization of the resulting cells.

McCain would ban scientists from using donor eggs to create disease - specific stem cell lines or chimeric animals to see how human stem cells behave during development.

Stem cell advocates have been expressing serious worry that ethical requirements spelled out in the draft guidelines — in particular, informed consent procedures for embryo donors — will rule out the use of many existing human embryonic stem cell lines, including the 21 lines approved under the Bush Administration.

The final guidelines on research with human embryonic stem cells issued on Monday by the National Institutes of Health set out criteria for determining which ES cell lines can be used in federally funded experiments and give NIH discretion to approve old lines that don't meet stringent modern ethical requirements.

Mindful of public sensitivities, Daley opted to pursue experiments using what he considers the least controversial human materials to create new nonpresidential stem cell lines — poor quality embryos and oocytes that, in his words, «otherwise would have been disposed of as medical waste.»

In May 2006, Eggan's lab received approval from Harvard to seek healthy human eggs from female donors, a first step toward using research cloning to create new stem cell lines.

A team of researchers in Germany and Denmark led by Steven Johnsen, Professor at the University Medical Center Göttingen, Germany, used human prostate cancer cell lines and depleted them of the DNA - binding protein CHD1.

The scientists used the new indexing method on several human cell lines and from a mouse brain to reveal the methylome of 3,282 single cells.

They used the gene editing technology CRISPR to engineer a series of human embryonic stem cell lines, which were identical apart from the number of DNA repeats that occurred at the ends of their HTT genes.

Derived mostly from human embryonic kidney 293T (HEK293T) and HeLa cell lines, EdiGene Knockout (KO) Cell Lysates have been optimized through the use of genome editing technology and validated at the genomic level through PCR and Sanger - sequencing techniques to ensure the accuracy and knockout of the target gcell lines, EdiGene Knockout (KO) Cell Lysates have been optimized through the use of genome editing technology and validated at the genomic level through PCR and Sanger - sequencing techniques to ensure the accuracy and knockout of the target gCell Lysates have been optimized through the use of genome editing technology and validated at the genomic level through PCR and Sanger - sequencing techniques to ensure the accuracy and knockout of the target gene.

In preclinical experiments using human prostate cancer cell lines, Fu's team showed that increased PLK1 expression activated an oncogene known as c - RAF, which has previously been shown to play a role in regulating cell growth and division.

In the lab, the scientific team used an approach that combined functional RNAi analysis with gene expression analysis in breast cancer - derived cell lines and in human breast cancers replicated in mice.

This rhinovirus reliably kills HeLa cells, a line of human - derived cells that have long been used in studies of viral infection.

Yet, «these studies using HeLa cells had to rely on information from the «reference» sequence produced by the Human Genome Project, even though there was evidence that the genomes of HeLa cell lines were probably quite different,» said Lars Steinmetz, Ph.D., who led the G3 study.

In August of last year, President George W. Bush announced that scientists who received public research money could use only the human embryonic stem - cell lines that already exist — a decision that dismayed many researchers.

His team have begun recolonising the primate scaffolds with human cells that line blood vessels, the first step towards human - scale biolimb development, and have started experiments using human myoblasts in rats instead of the mice ones.

The goal of the first experiment was to see whether lncRNAs are differentially expressed in prostate cancer by measuring total RNA from prostate cancer cell lines and normal epithelial prostatic cells using NCode human ncRNA array and SurePrint G3 human lncRNA microarrays.

Palanichamy, Chakravarti and their colleagues conducted this study using 13 primary GBM cell lines derived from patient tumors, four commercially available GBM cell lines and normal human astrocyte cells.

Its first target was the regulatory network involved in controlling the differentiation of THP - 1 cells, a line of human leukemia cells used in laboratory experiments.

«The use of nonhuman oocytes for SCNT is currently the only ethically justifiable option given the large numbers of eggs required to derive cloned human stem cell lines,» he said.

Nonetheless, passage of the measure marks a significant milestone in efforts to overturn the Bush policy, which limits government funding to research using 60 human ES cell lines derived before 9 August, 2001.

L: We use adult stem cells, and we use the human embryonic cell lines that the government has already approved.

Twenty - seven human embryonic stem cell (hESC) lines are ethically derived and should be approved for use in research funded by the US linkurl: National Institutes of Health; http://www.nih.gov/ (NIH), a committee advising the NIH director linkurl: recommended today (December 4).

Only a few laboratories in the world are attempting this technique in human stem cell research and, thus far, no human stem cell lines have been derived using this method.

What Collins does not say, however, is that the new NIH guidelines also allow for federal funds to be used in studying new human embryonic stem cell lines that are created (by private entities, of course) beyond the 700 currently in existence.

The new NIH guidelines do not permit the use of federal funds for creating new human embryonic stem cell lines.

Human primary hepatocytes and immortalized cell lines (e.g., HepaRG) are used to study these disorders, but due to these cells» high variability, low availability, short assay window, or lack of physiological relevance, researchers studying metabolic diseases are searching for more robust and reliable models.

\ n \ nIn other words this is a political attempt, based on a technicality, to advance science in the field by getting the US Administration to greatly expand the number of human ES - cell lines that can be used under federal funding.

Using pre-implantation genetic diagnosis derived human embryonic stem cell lines (hES) or human induced pluripotent stem cells carrying the causal mutation for neuro - muscular diseases, our objective is double: identify new physiopathogical mechanisms and develop new therapeutic strategies.

First, cells from Cellartis Human iPS Cell Line 22 (ChiPSC22) were modified to stably express AcGFP1, then these cells were cultured using the DEF ‑ CS culture system in a 48 - well plate.

In the present study, the researchers have developed a mass spectrometry - based method that is sensitive and reproducible in order to measure, at steady - state conditions, absolute protein copy numbers across human tissues and cell lines and compared these levels with the corresponding mRNA levels using transcriptomics.

The use of higher resolution techniques, such as array CGH and, soon, whole genome sequencing, will enhance the ability of researchers to examine stem cell lines to determine which are best — least likely to result in diseases and other problems — to use in creating therapies for use in humans.

Stem cell researchers from UCLA used a high resolution technique to examine the genome, or total DNA content, of a pair of human embryonic stem cell lines and found that while both lines could form neurons, the lines had differences in the numbers of certain genes that could control such things as individual traits and disease susceptibility.

In addition to our computational work, we run a small wetlab where we use CRISPR - Cas genome editing in human cell lines to obtain a deeper understanding of how genetic variants affect the cell.

Using cutting - edge techniques enabled by next - generation sequencing, the authors generated complete methylome maps at single nucleotide resolution in a low - passage breast cancer cell line and normal breast tissue (primary human mammary epithelial cells).

In an attempt to solve these issues and to follow up on the «Call for Standardized Naming and Reporting of Human ESC and iPSC Lines» (1), a tool was created to use identifying information to generate a unique cell line name.

For more on human embryonic stem cells, see Teisha Rowland's «All Things Stem Cell post on «Induced Pluripotent Stem Cells: A New Stem Cell Line with a Long History,» The National Institute of Health's Stem Cell FAQs, or, for a visual explanation of terms used, see All Things Stem Cell's Visual Stem Cell Gloscells, see Teisha Rowland's «All Things Stem Cell post on «Induced Pluripotent Stem Cells: A New Stem Cell Line with a Long History,» The National Institute of Health's Stem Cell FAQs, or, for a visual explanation of terms used, see All Things Stem Cell's Visual Stem Cell GlosCells: A New Stem Cell Line with a Long History,» The National Institute of Health's Stem Cell FAQs, or, for a visual explanation of terms used, see All Things Stem Cell's Visual Stem Cell Glossary.

Conditions for hypoxia / reoxygenation cycles were optimized using two human metastatic breast cancer cell lines (MDA - MB 231 and BCM2).

Western blot analysis of antibody specificity has been done using a routine sample setup composed of IgG / HSA - depleted human plasma and protein lysates from a limited number of human tissues and cell lines.

In an executive order, Bush stated that federal funds could only be used for research on human embryonic stem cell lines that had already been established (only 22 cell lines).

Research using human induced Pluripotent Stem Cells (iPSC) and human Embryonic Stem Cell (hESC) lines is not covered by the Human Fertilisation and Embryology Act (2human induced Pluripotent Stem Cells (iPSC) and human Embryonic Stem Cell (hESC) lines is not covered by the Human Fertilisation and Embryology Act (2human Embryonic Stem Cell (hESC) lines is not covered by the Human Fertilisation and Embryology Act (2Human Fertilisation and Embryology Act (2008).