Sentences with phrase «used primary cultures»
To begin to test this hypothesis, we first used primary cultures of the E14 rat ventral midbrain (VM), which are an experimental tractable model of mDA neurons that are widely used to study the molecular mechanisms regulating mDA neurite growth [18].
http://www.neuronalsignaling.org/ Not exact matches
Besides the conditions of society itself, under which family and friends had primary responsibility for the care of the dying and the dead, memento mon were spread throughout culture: in the church's art, in morality plays like Everyman, in drinking songs, in the ordinary artifacts of everyday life (e.g., in Austria a towel hanger portraying a human form split down the middle: one half a beautiful young woman, the other a skeleton) To be sure, the specter of death (and judgment) has been used as a form of social control.
He is also developing a robust and comprehensive panel of 3 - D cell culture models from patient - derived primary cells that can be used to characterize different disease phenotypes and investigate the chemo - response of cells to novel or known drugs.
To overcome these limitations, we used two - color flow cytometry to identify and select microvascular endothelial cells from primary cultures obtained from different organs of mice whose tissues harbor a temperature - sensitive SV40 large T antigen (H - 2Kb - tsA58 mice; ImmortoMice).
«An important, overarching goal of the field is to develop culture systems using primary cells with minimal ex vivo [external] manipulation.
Efforts to promote a selective increase in the number of endothelial cells present in primary cultures by using different media preparations (MCDB - 131, D - Valine, etc.) were largely unsuccessful.
Pilot studies using a variety of strategies suggested that this goal could be best achieved by stimulating primary organ cell cultures with TNF - α (10 ng / ml) and targeting cells expressing the endothelial cell adhesion molecules E-selectin and VCAM - 1 through a FACS - driven strategy.
Once primary cultures were established, the cells were immortalized with human papilloma virus E6 and E7 oncogenes using the disabled recombinant retroviral vector pLXSN16E6 / E7 (26).
To address these different topics we are developing relevant animal models that will be used in combination with primary neuronal cultures, rodent brain slices, and brain tissues.
We next sought to determine whether transfection with the miR - 181a inhibitor could also promote neurite growth in primary cultures of E14 VM neurons, which are widely used models of mDA neurons [18].
We use a combination of conditional mouse models, primary cell culture (human and mouse), live microscopy, highthroughput genomics and proteomics approaches, and basic molecular biology.
Although this possibility has not yet attracted as much attention as the ideas of cell replacement, personalized medicine and other more direct clinical applications, hESCs are expected to be superior to most commonly used cell - culture models of drug discovery which employ tumor - derived or immortalized cell lines or primary cell culture.
While some herbs or flowers may be used to brew KT in the primary fermentation, they don't contain enough of what the culture needs to sustain itself over time.
Matcha may be used in primary but in our experience does not produce good offspring, so use a culture from your SCOBY Hotel.
Kate Copping - Westgarth Primary School, Victoria Using Data to Develop Collaborative Practice and Improve Student Learning Outcomes Dr Bronte Nicholls and Jason Loke, Australian Science and Mathematics School, South Australia Using New Technology for Classroom Assessment: An iPad app to measure learning in dance education Sue Mullane - Sunshine Special Developmental School, Victoria Dr Kim Dunphy - Making Dance Matter, Victoria Effective Differentiation: Changing outcomes in a multi-campus school Yvonne Reilly and Jodie Parsons - Sunshine College, Victoria Improving Numeracy Outcomes: Findings from an intervention program Michaela Epstein - Chaffey Secondary College, Victoria Workshop: Developing Rubrics and Guttman Charts to Target All Students» Zones of Proximal Development Holly Bishop - Westgarth Primary School, Victoria Bree Bishop - Carwatha College P - 12, Victoria Raising the Bar: School Improvement in action Beth Gilligan, Selina Kinne, Andrew Pritchard, Kate Longey and Fred O'Leary - Dominic College, Tasmania Teacher Feedback: Creating a positive culture for reform Peta Ranieri - John Wollaston Anglican Community School, Western Australia
Todd Whitaker and Steve Gruenert's excellent book School Culture Rewired: How to Define, Assess, and Transform It makes a powerful case for why focusing on culture is our primary concern as site leaders: «Culture is not a problem that needs to be solved, but rather a framework that a group can use to solve problems» Culture Rewired: How to Define, Assess, and Transform It makes a powerful case for why focusing on culture is our primary concern as site leaders: «Culture is not a problem that needs to be solved, but rather a framework that a group can use to solve problems» culture is our primary concern as site leaders: «Culture is not a problem that needs to be solved, but rather a framework that a group can use to solve problems» Culture is not a problem that needs to be solved, but rather a framework that a group can use to solve problems» (p. 6).
One of the primary characteristics of students in the millennial culture is their comfort with and consistent use of technology on a daily basis (Parette, Huer, & Scherer, 2004; U.S. Department of Education, 2004).
Get Smart with Art @ the de Young is an interdisciplinary curriculum package that uses art objects as primary documents, sparking investigations into the diverse cultures represented by the Museum's collections.
Through the use of textbook and document - based primary and secondary source readings, interactive activities, historical fiction, videos, and research projects, the students develop a rich understanding of the varying cultures while becoming more adept at critical thinking and analysis, note taking, research, essay writing, class discussion, and both independent and collaborative study skills.
✴ Well designed digital content based social studies courses allow students to engage actively with the big ideas of history, geography, culture, and civics through the use of interactive visual media that incorporate primary and secondary source documents and media that «bring the powerful ideas of social studies to life.»
Instead, they take their own culture and heritage as primary sources of inspiration and combine photography with other techniques such as collage, silk - printing, painting, and images that use cultural ephemera and archival documents.
Leaders in child health care recommend primary care physicians use a parent - centered approach, with a focus on the parent — child relationship in the context of family, culture, and community.