Sentences with phrase «used splenocytes»

Not exact matches

T cells from mouse splenocytes were purified with magnetic naive CD8 + T cell isolation kits using instructions provided by the manufacturer (Stemcell Technologies, Vancouver, Canada).

Additionally, an aliquot of 4 × 106 splenocytes was stained for Foxp3 expression using methods and reagents, provided by the manufacturer (eBioscience), prior to flow cytometric analyses.

In total, 106 splenocytes were cultured without peptide or with 0.2 μM LCMV gp33 peptide, NP396 peptide for 5 h. IFN - γ positivity by flow cytometry was used to identify LCMV - specific cells (13).

Once optimal detector gains were determined, an extensive brightness index of dyes was established on this instrument using sequential single staining of mouse splenocyte with 43 different antibody conjugates against mouse CD8a (53 - 5.8).

We used Rag1 - / - mouse splenocytes, a source of ILC3s, because the absence of T cells in this model makes ILC3s the dominant IL -22-expressing cell type [40].

For mouse ILC3s, splenocytes from Rag1 - / - mice were sorted (Lin -(CD3, CD45R, CD11c, GR - 1, NK1.1) c - kit + Thy1.2 +) and cultured as described for human cells using recombinant mouse cytokines.

Additionally, splenocytes of recipient animals were used as responder cells in mixed lymphocyte reactions and ELISPOT to determine responses to donor alloantigen.

In addition, mixed lymphocyte cultures using the cells of the chimeric animals as responder cells to donor splenocytes failed to demonstrate any sensitization or clonal deletion of alloreactive T cells; i.e. T cell responses were identical to those of control animals (n = 4)(data not shown).

Balb / c splenocytes were used as third - party responder cells assuming that these would induce the same degree of T cell proliferation in tolerant and control animals.

One of the assays widely used in the literature is the application of splenocytes from tolerant animals as responder cells in a 4 - day mixed lymphocyte culture.