Sentences with phrase «uses epitopes»

The Sette lab's approach uses epitopes as specific probes to define the immune signatures associated with productive / protective immunity versus deficient immunity / immunopathology.

Protein complexes isolated through affinity purification of a bait protein using an epitope tag and competitive elution are separated through blue native electrophoresis.

Not exact matches

Together with the company Pepscan (Lelystad / The Netherlands) and the Leibniz Institute for Pharmacology in Berlin an in - depth analysis of the binding epitopes was performed using peptide chemistry and NMR spectroscopy.

The algorithm was built using tools the researchers developed to define how T cell receptors recognize a part of the antigen called the epitope.

We have turned the problem on its head and only use previously - tested epitopes.

Like the Rosetta Stone that scholars used to decode hieroglyphics, researchers trained the algorithm with more than 4,600 T cell receptors and then used it to correctly assign 81 percent of the human T cells and 78 percent of mouse T cells to one of 10 different viral epitopes.

They then designed two DNA strings that coded for these epitopes and used them to genetically immunize different groups of mice.

The researchers identified 5 toxins within the snake venom and used a technique called SPOT - synthesis to identify the sections of the toxin (epitopes) that are recognized by coral snake antivenom antibodies.

Some examples from their lab include using AAV to introduce epitope tags into the endogenous alleles of the p53 and PTEN tumor suppressor genes in human cells (Kim et al 2008).

This antibody binds to the D664KW666 core 2F5 epitope but use entirely different germ line V genes from 2F5 and that are significantly less divergent than 2F5 from thei

We also turned our attention to HIV - 1 and found several such epitopes in the envelope (Env) protein of HIV - 1, one of which was included in both the HIV - 1 E and HIV - 1 B Env antigens that were used in the «moderately effective» HIV RV144 trial in Thailand.

Using JanusMatrix, we have identified T cell epitopes in H7N9 influenza HA protein that are highly conserved with human genome epitopes, and these epitopes possess low immunogenicity, activate natural Tregs and suppress bystander effector T cell responses in vitro.

Predicted epitopes for use in ELISPOT, tetrameric assays and for intracellular cytokine assays

After extensive washing with immunoprecipitation lysis buffer, the immunoprecipitated proteins were analyzed by immunoblotting using specific antibodies against DDX3 and FLAG epitope (ab1257, Abcam, Cambridge, United Kingdom).

The project aims to develop an influenza vaccine that educates the immune system to recognise cross-reactive epitopes by diluting out the strain specific epitope (Epitope Dilution Phenomenon), using a combination of influenza hemagglutinin antigens delivered on virus - like paepitope (Epitope Dilution Phenomenon), using a combination of influenza hemagglutinin antigens delivered on virus - like paEpitope Dilution Phenomenon), using a combination of influenza hemagglutinin antigens delivered on virus - like particle.

The iCubed runs a training session per year — following these training sessions, NTD researchers can gain access to a dedicated website containing their genomes of choice for their own epitope - mapping use.

Modern Cytological Approaches Including Epitope Tagging and Imaging Yeast Cells Using Fluorescence Microscopy

We first examined differences between B6 Prf1 − / − HLH responses in naive mice and mice immunized against the CD8 LCMV epitopes gp33 and NP396 using a well - characterized DC immunization protocol (15).

The overall score encompass several factors: reproducibility of the antibody staining in different cell lines (and if signal strength correlates with RNA expression levels); assays for enhanced antibody validation by using antibodies binding to different epitopes on the same target protein (independent antibodies), by knockdown / knockout of the target protein (genetic methods) and by matching of the signal with a GFP - tagged protein (recombinant expression); experimental evidence for location described in literature.

From those studies he and his collaborators further developed the notion that different MHCs have distinct binding specificities that can be used to predict epitopes.

Annotated protein expression profiles are performed using single antibodies as well as independent antibodies (two or more independent antibodies directed against different, non-overlapping epitopes on the same protein).

The laboratory is defining in chemical terms the specific structures (epitopes) that the immune system recognizes, and uses this knowledge to measure and understand immune responses.

These efforts have resulted in the discovery of previously unknown human T cell epitopes that may be key in the initiation of allergic responses, and to the development of biochemical and bioinformatic tools that have been used to map the human T cell response to a large panel of common allergens.

In the model of herpes simplex virus (HSV)-1 infection of B6 mice, > 90 % of the responding CD8 + T cells use Vbeta10 or Vbeta8 and are directed against a single glycoprotein B (gB498 - 505) epitope, gB - 8p.

This approach is considered «passive,» as nanoparticles do not use ligands targeting specific epitopes on the cell surfaces.

Epitope mapping of a selection of antibodies using peptide arrays containing 175 000 peptides covering hundreds of proteins.

New monoclonal antibodies and epitope mapping using recombinant p53.

Using an integrative approach that combined electron microscopy on the Env trimer complex with PGT151 (led by the Ward lab) with the structure of the PGT151 Fab by x-ray crystallography (led by the Wilson lab), the scientists were able to visualize the location of the PGT151 - series binding site on the Env trimer — which includes a spot on one gp41 protein with two associated sugars (glycans), a patch on the gp120 protein and even a piece of the adjacent gp41 within the trimer structure — «a very complex epitope,» said Claudia Blattner, a research associate in the Wilson laboratory at TSRI and member of the IAVI Neutralizing Antibody Center who, along with graduate student Jeong Hyun Lee, was a first author of the second paper.

Using sera from malaria - immune adults, it has been shown that the expressed recombinant proteins contained heat - labile epitopes suggesting that they adopt their native conformation and are likely to be biochemically active.

By using sera from malaria - exposed immune adults, the immunoreactivity of 20 recombinant proteins was assessed, and most of the merozoite ligands were found to carry heat - labile epitopes.

In fact, whey hydrolysate is even used in baby formula because of its fast - acting power and lack of allergenic epitopes.