The Cancer Genome Project
uses human genome sequence and mutation detection techniques to find changes in DNA involved in the development of human cancers.
Not exact matches
Dec. 18, 2013 — The most complete
sequence to date of the Neanderthal
genome,
using DNA extracted from a woman's toe bone that dates back 50,000 years, reveals a long history of interbreeding among at least four different types of early
humans living in Europe and Asia at that time, according to University of California, Berkeley, scientists.
When the
human genome draft was announced 10 years ago, many researchers and policymakers anticipated
using the newly
sequenced code to develop a wealth of diagnostic and treatment capabilities.
«We explored the opportunity of
using sperm RNA elements as a predictor of
human health, with applications at the fertility clinic that would go hand - in - hand with the new neonatal intensive care unit genome sequencing to better health outcomes,» said Dr. Krawetz, associate director of the C.S. Mott Center for Human Growth and Develop
human health, with applications at the fertility clinic that would go hand - in - hand with the new neonatal intensive care unit
genome sequencing to better health outcomes,» said Dr. Krawetz, associate director of the C.S. Mott Center for
Human Growth and Develop
Human Growth and Development.
Using metabolic function analyses of identified genes, we compared our
human genome with the average content of previously
sequenced microbial
genomes.
Using advanced
sequencing technologies, University of Oklahoma anthropologists demonstrate that
human DNA can be significantly enriched from dental calculus (calcified dental plaque) enabling the reconstruction of whole mitochondrial
genomes for maternal ancestry analysis — an alternative to skeletal remains in ancient DNA investigations of
human ancestry.
The goal of the
human genome project was to
use DNA
sequencing to reveal all three billion DNA letters in our chromosomes and find all our genes.
They digested
human genomic DNA
using Cas9 nucleases in a test tube, which was then subjected by whole
genome sequencing.
I always
used to say we should treat the
sequencing of the
human genome like income tax: It's criminal to evade it, but there are legal means of avoiding it.
The millions of base pairs of
sequence that Genovese and McCarroll's team have located will be added to the next release of the reference
human genome assembly — the «Google maps» of the
human genome that geneticists
use every day — providing a more comprehensive view of the
genome and how the pieces all fit together.
As when he worked on the
human genome, Venter is relying on a radical technique called shotgun
sequencing: He chops up vast amounts of DNA into tiny pieces and then
uses sophisticated computer analyzers to piece them back together into intelligible genes and chromosomes.
In order to locate all gene switches, the Freiburg research team
used modern
sequencing methods to examine the entire
genome — DNA, epigenetic markers and RNA — during the development, maturation and disease of
human cardiac muscle cells.
The research team from the National
Human Genome Research Institute (NHGRI) and the National Cancer Institute (NCI), both parts of NIH, extended their recent genome sequencing study of skin bacteria, using DNA sequencing techniques optimized for identifying
Genome Research Institute (NHGRI) and the National Cancer Institute (NCI), both parts of NIH, extended their recent
genome sequencing study of skin bacteria, using DNA sequencing techniques optimized for identifying
genome sequencing study of skin bacteria,
using DNA
sequencing techniques optimized for identifying fungi.
Evolutionary geneticists Svante Pääbo, Johannes Krause, and colleagues at the Max Planck Institute for Evolutionary Anthropology in Leipzig, Germany, ground up a 30 - milligram sample and extracted and
sequenced all of the 16,569 base pairs of its mtDNA
genome,
using new techniques Pääbo's group has successfully employed to
sequence both Neandertal and prehistoric modern
human DNA.
Over the next year, the company plans to
use data from
human genomes sequenced with its new machines to develop the statistics needed to evaluate how accurate the data are.
Derived mostly from
human embryonic kidney 293T (HEK293T) and HeLa cell lines, EdiGene Knockout (KO) Cell Lysates have been optimized through the
use of
genome editing technology and validated at the genomic level through PCR and Sanger -
sequencing techniques to ensure the accuracy and knockout of the target gene.
Yet, «these studies
using HeLa cells had to rely on information from the «reference»
sequence produced by the
Human Genome Project, even though there was evidence that the
genomes of HeLa cell lines were probably quite different,» said Lars Steinmetz, Ph.D., who led the G3 study.
In 1991 he invented a quick way to find
human genes, using expressed sequence tags, after the senior scientists at the Human Genome Project had said it wouldn't
human genes,
using expressed
sequence tags, after the senior scientists at the
Human Genome Project had said it wouldn't
Human Genome Project had said it wouldn't work.
All the analysts and pundits were saying that
genome sequencing was dead, there's no
use for it anymore — the
human genome has been
sequenced.
The company's thermal cyclers were
used by every major
genome centre during the
sequencing of the
human genome and were most recently
used by the BC Cancer Agency during the
sequencing of the SARS virus.
The new NIST
human genome RMs increase the ability of DNA
sequencing laboratories to be more confident in their reporting of true positives, false positives, true negatives and false negatives, and therefore, significantly improve genetic tests
used for disease risk prediction, diagnosis, and progression tracking.
Using a single pollinated pine seed, the team assembled the largest
genome ever
sequenced: 22.18 billion base pairs, more than seven times longer than the
human genome.
Together with the French gene
sequencing center Genoscope, in the Paris suburb of Evry, Celera will perform the initial
sequencing using the «whole -
genome shotgun» approach it unleashed on the
human genome.
Using whole exome
sequencing (a next generation test to analyze the exons or coding regions of thousands of genes simultaneously) conducted at the Baylor College of Medicine Human Genome Sequencing Center, the researchers identified CLP1 mutations in two unrelated families with the
sequencing (a next generation test to analyze the exons or coding regions of thousands of genes simultaneously) conducted at the Baylor College of Medicine
Human Genome Sequencing Center, the researchers identified CLP1 mutations in two unrelated families with the
Sequencing Center, the researchers identified CLP1 mutations in two unrelated families with the disorder.
Using the new technology, Complete Genomics» chief scientist Radoje Dramanac estimates that the company will soon be able to
sequence a complete
human genome in about a day.
In particular, the Neandertal
genome sequence can now be
used to catalog changes that have become «fixed» (are invariant within a population or species) in modern
humans during the last few hundred thousand years and should be helpful for identifying genes affected by positive selection since
humans diverged from Neandertals.
To go a step further, researchers are
using more powerful
genome - wide
sequencing to take a detailed look at those viruses that resemble known threats to
humans.
Today, the National
Human Genome Research Institute (NHGRI) in Bethesda, Maryland, announced it will pour more than $ 116 million into three
sequencing centers, which will
use «large - scale»
sequencing to focus on unraveling the sources of disease and elucidating the workings of disease - causing organisms.
Using a specific work flow, they assessed both the coding and noncoding regions of the
human genome, including the evaluation of highly polymorphic SNPs, structural and copy number variations, as well as 69 control
genomes sequenced by the same procedures.
Using the new approach in the hydatidiform
genome, the researchers were able to identify and
sequence 26,079 segments that were different from a standard
human reference
genome used in
genome research.
For example, the large - scale
sequencing program has already started to
sequence the
genomes of 100 microorganisms found in the
human gut, and will build on this by
using genomic
sequencing to characterize the complex microbial communities found at many sites in and on the
human body.
In order to locate all gene switches, the Freiburg research team
used modern
sequencing methods to examine the entire
genome - DNA, epigenetic markers and RNA - during the development, maturation and disease of
human cardiac muscle cells.
NORMAN —
Using advanced
sequencing technologies, University of Oklahoma anthropologists demonstrate that
human DNA can be significantly enriched from dental calculus (calcified dental plaque) enabling the reconstruction of whole mitochondrial
genomes for maternal ancestry analysis — an alternative to skeletal remains in ancient DNA investigations of
human ancestry.
We will
use a lab - adapted
human strain of rotavirus with a known
genome sequence to test and optimize this
sequence - independent amplification method.
Chief among the restrictions that accompanied the X Ten was the requirement that X Tens only be
used for whole -
genome sequencing, and only for
human genomes.
This flash animation shows the processes involved in the Sanger
sequencing method — the DNA
sequencing method
used during the
Human Genome Project.
I will review recent advances in the
use of nanopore
sequencing for clinical microbiology and
human genetics, including our collaborations on viral and bacterial diagnostic
sequencing, real - time surveillance, direct RNA and
human whole -
genome sequencing, and discuss the opportunities and barriers around moving to
sequencing as a routine test in the clinic.
The Cancer
Genome Project at the Wellcome Trust Sanger Institute is part of an international project
using DNA
sequencing to identify mutations in genes critical to the development of
human cancers.
With the effort to
sequence the entire
human genome speeding toward completion, some researchers are now focusing their energy on developing the next generation of tools that can be
used to extract...
Biological Annotation of the Genomic
Sequence A key use of the sequence information from the canonical model organisms, such as Drosophila, will be to help interpret the sequence of the human
Sequence A key
use of the
sequence information from the canonical model organisms, such as Drosophila, will be to help interpret the sequence of the human
sequence information from the canonical model organisms, such as Drosophila, will be to help interpret the
sequence of the human
sequence of the
human genome.
The
use of higher resolution techniques, such as array CGH and, soon, whole
genome sequencing, will enhance the ability of researchers to examine stem cell lines to determine which are best — least likely to result in diseases and other problems — to
use in creating therapies for
use in
humans.
We
use genome sequences to advance understanding of the biology of
humans and pathogens to improve
human health
Here at the Wellcome Sanger Institute, we
use genome sequences to better understand
human and pathogen biology to improve
human health.
A few interesting articles in early life
human microbiome, plus: A comparison between Staphylococcus epidermidis commensal and pathogenic lineages from the skin of healthy individuals living in North American and India; A new tool to reconstruct microbial
genome - scale metabolic models (GSMMs) from their
genome sequence; The seasonal changes in Amazon rainforest soil microbiome are associated with changes in the canopy; A specific class of chemicals secreted by birds modulates their feather microbiome; chronic stress alters gut microbiota and triggers a specific immune response in a mouse model of colitis; and evidence that the short chain fatty acids profile in the gut reflects the impact of dietary fibre on the microbiome
using the PolyFermS continuous intestinal fermentation model.
Using its proprietary
sequencing instruments, chemistry, and software, the company has
sequenced more than 20,000 whole
human genomes.
We have a variety of data, both from actual assemblies and simulation studies, which show that about 96 % of the reference
human genome is addressable
using this library and
sequencing strategy, including a significant fraction of the high - copy repeat
sequences in the
genome.
Tracing the Route of Modern
Humans out of Africa by
Using 225
Human Genome Sequences from Ethiopians and Egyptians.
Very good for giving the overview of the BioTech industry as it applies to the
Human Genome sequencing race and the potential for
use of this information in the future for Personalized Medicine.
ChIP - Seq
sequencing reads were mapped to the
human genome (GRCh37 / hg19)
using Bowtie2 with default parameters (v2.1.0, [27]-RRB-.
By the mid-1990s, when the
Human Genome Project was in full swing, scientists were
sequencing DNA
using capillary sequencers.