Sentences with phrase «uses liquid chromatography»

They used Liquid chromatography — mass spectrometry (LC - MS), which combines the physical separation capabilities of liquid chromatography with the mass analysis capabilities of mass spectrometry (MS) to isolate the chemical constituents of virgin coconut oil (VCO).

With this labeling method, the intrinsically labeled Golden Rice β - carotene showed a protonated molecule of m / z of Mβc + H + = 536 + 1 (representing unlabeled β - carotene) and a range of isotopomers with the most abundant showing an enrichment of 9 deuterium at Menrich - βc = Mβc + H + + 9 mass units (Figure 4) as analyzed by using the liquid chromatography / mass spectrometry with a positive atmospheric pressure chemical ionization interface (the total m / z was Mβc + 9 + H + = 536 + 9 + 1 = m / z 546)(23).

The researchers used liquid chromatography - mass spectrometry to analyse the collected samples, as well as investigating the impact of different breathing patterns.

The researchers milked wasps for their venom and then analyzed the components using liquid chromatography and mass spectrometry.

Using liquid chromatography - mass spectrometry (a process that separates complex mixtures and analyzes ingredients by measuring a weight - to - charge ratio), researchers compared the constituent chemicals in the food to standards for common molds, heavy metals and ethylene glycol (or antifreeze, which Goldstein says is the number one cause of kidney failure).

Metabolite analyses were done using liquid chromatography coupled with mass spectrometry.

The WSU researchers used liquid chromatography - mass spectrometry to test the samples for nicotine and other plant - based drugs like caffeine and atropine, a muscle - relaxant.

They used liquid chromatography and tandem mass spectroscopy to identify proteins in the complex.

Proteomic datasets were obtained using liquid chromatography tandem mass spectrometry (LC - MS / MS).

In this study, we collected representative food samples and analyzed the choline concentration of 145 common foods using liquid chromatography - mass spectrometry.

Exposure was confirmed by isolation of moxidectin from a biopsy specimen of adipose tissue, using liquid chromatography - mass spectroscopy methods.

Not exact matches

They also monitor the heat of the batch, and of the oleoresin added, by using high performance liquid chromatography (HPLC) testing.

Improved method for quantifying capsaicinoids in capsaicin using high performance liquid chromatography.

Furthermore, tests using a new test called liquid chromatography - mass spectrometry (LC - MS)-- scientists believe it is a more accurate way to measure gluten in fermented beers — showed that even though all the tested beers «had been rated by their makers to have gluten levels below 20 mg / kg, according to ELISA», the new test «found detectable gluten fragments in every sample using LC - MS, and most had much higher levels of gluten than ELISA detected.»

Now, High Performance Liquid Chromatography is used to determine parts per million of capsaicin, which is then converted into Scoville Heat Units.

As Dave noted back then, the report was interesting for the lack of detail of the methodology used to calculate Scoville Units through HPLC (High Performance Liquid Chromatography).

A recent sample of this rare Bangladeshi chilli was tested * using High - performance liquid chromatography and registered a mind blowing 1,598,227 Scoville Heat Units (SHU), that's 3 times hotter than the Red Savina Habanero or 300 times hotter than a Jalapeno pepper!

Total urinary As was measured by collision cell ICP - MS using germanium as an internal standard.30 Arsenic species were obtained by a high - performance liquid chromatography GP50 pump (Dionex Corp) and a PRP100X column (Hamilton Co) connected to a collision cell ICP - MS.29, 31 - 33 Approximately 10 % of samples were run in duplicate for both analyses.

For products with As concentrations of more than 300 ng / g, a different lot number sample was purchased and tested using ICP - MS for total As and high - performance liquid chromatography with ICP - MS for As species.

They analyzed those samples using a chemical detection technique known as liquid chromatography mass spectrometry that singles out the presence of targeted chemicals.

The process involving liquid chromatography - electrospray ionization - tandem mass spectrometry relies on a vital step called «charge derivatization» or using a permanently charged reagent to selectively trap the estrogens and isolate them from the lipids and proteins that could interfere with estrogen detection, Schug said.

The new methods involve separation of the hydroperoxides using either liquid or gas chromatography and detection using mass spectrometry.

The team used a nanoflow liquid chromatography instrument to sort the molecules in the meteorite sample, then applied nanoelectrospray ionization to give the molecules an electric charge and deliver them to a high - resolution mass spectrometer instrument, which identified the molecules based on their mass.

Milk and urine samples were analyzed for glyphosate and glyphosate metabolites using high sensitivity liquid chromatography - mass spectrometry methods specifically optimized for the task.

These include: acid hydrolysis and monosaccharide composition using gas - liquid chromatography [3], microscopic observation of xylem stem sections [4, 5], seedling growth on medium containing cell wall hydrolyzing enzymes [6] and Fourier - Transform Infrared (FT - IR) microspectroscopy [7, 8].

Methods: The researchers used capillary liquid chromatography - mass spectrometry to extensively characterize proteins present in the Salmonella wild - type and mutant strains.

Reversed - phase high - performance liquid chromatography (HPLC) with electrochemical detection was used to analyse ascorbic acid, based on the method of Iriyama et al. with modifications [29], [30].

In MudPIT, a complex mixture of proteins and peptides are separated using 2 - D liquid chromatography, and then the separate proteins are digested (chopped into smaller «peptide» pieces with enzymes) and subjected to the sensitive technique of mass spectrometry (which identifies the components based on their masses).

The researchers used an innovative open - access bioinformatics pipeline developed by the group to process and annotate liquid chromatography / mass spectrometry data.

Liquid chromatography mass spectrometry (LCMS) was carried out using a Shimadzu LC - 20AD spectrometer at The Scripps Research Institute (La Jolla, CA, USA), and electrospray ionization (ESI) mass analysis with a Thermo Scientific LTQ Orbitrap - XL spectrometer at the Salk Institute.

The authors of this eBook explored direct analysis of bioreactor harvest using capillary sodium - dodecyl sulfate (cSDS) electrophoresis, size - exclusion high - performance liquid chromatography (SE - HPLC), and surface plasmon resonance (SPR) assays.

He analyzed the water using a method called high - performance liquid chromatography.

Brains that have been voxelated into ~ 700 cubes will be prepared using automated microscale sample processing and analyzed using high - throughput reversed - phase liquid chromatography coupled online with high - resolution mass spectrometry to obtain both the spatial localization and relative abundance of brain proteins.

The summary below highlights the applications of commonly used custo... Keywords: Extinction coefficient, Gene expression, High performance liquid chromatography, Microarray Analysis, Polymerase chain reaction - quantitative

Methods: The researchers used capillary liquid chromatography - mass spectrometry analyses to analyze the proteomes of Salmonella mutant strains lacking specific RNA - binding proteins that mediate the translation of RNA into protein (post-transcriptional control) and the parent strain grown under four different conditions.

Her doctoral research examined novel separations of nucleic acids using ion - pairing reversed - phase high performance liquid chromatography (IP - RPLC).

Here we use a metabolic labelling approach to achieve rapid generation of bio-isotopologues of the complete Caenorhabditis elegans transcriptome and its modifications and use them as reference standards to characterise the RNA modification profile in this multicellular organism through an untargeted liquid - chromatography tandem high - resolution mass spectrometry (LC - HRMS) approach.

High - performance liquid chromatography was used to tracked catechin concentrations.

As discussed in Part II of this review, urine hormone testing using both gas chromatography - mass spectrometry (GS - MS) or liquid chromatography - tandem mass spectrometry (LC - MS / MS) is well - established in the medical literature as a reliable method of assessing levels of active (free & conjugated) hormones and their metabolites, correlates well with patient symptoms, and reflects the beneficial impact (or lack of benefit and potentially oncogenic effect) of therapeutic interventions.43, 444 Radioimmunoassay (RIA) and enzyme - linked immunosorbent assay (ELISA, also known as enzyme immunoassay [EIA]-RRB- analysis may not be accurate.

Additionally, DHT, which is very difficult to measure in any medium, is most accurately measured in serum using high - performance liquid chromatography - tandem mass spectrometry (LC - MS / MS).5, 6

Measuring steroid hormones via urine testing using both gas chromatography - mass spectrometry (GS - MS) or liquid chromatography - tandem mass spectrometry (LC - MS / MS) has been shown to produce highly accurate results, but radioimmunoassay (RIA) and enzyme - linked immunosorbent assay (ELISA, also known as enzyme immunoassay [EIA]-RRB- analysis may not be accurate.

Blood samples were collected and analyzed for plasma buprenorphine concentrations using ultra high pressure liquid chromatography / mass spectrometry.

Qualitatively analyzed synthesized compounds for purity using Mass Spectrometry (MS), Liquid Chromatography - Mass Spectrometry (LC - MS), Thin Layer Chromatography (TLC), Nuclear Magnetic Resonance (NMR), and SDS - PAGE.

Purification of oligonucleotide - based medicines using high performance liquid chromatography, ultra filtration, diafiltration, and freeze drying.

Chemist 5/1/2011 — 5/1/2012 XYZ Labs — Detroit, MI Conducted experiments, using Mathematica and Spartan ’10 to analyze data Performed sample analysis using both liquid and gas chromatography methods Successfully advocated for continuing education for all chemist and lab assistants

* Experience with sample preparation, titrations, micro-scale distillations, chemical synthesis and chemical analysis using instruments AA, ICP, UV - Vis, GC and GC - MS, and different liquid chromatography techniques.

Professional Duties & Responsibilities Biomedical and biotechnology engineer with background in design of biomaterials, biosensors, drug delivery devices, microfrabrication, and tissue engineering Working knowledge of direct cell writing and rapid prototyping Experience fabricating nanocomposite hydrogel scaffolds Proficient in material analysis, mechanical, biochemical, and morphological testing of synthetic and biological materials Extensive experience in bio-imaging processes and procedures Specialized in mammalian, microbial, and viral cell culture Working knowledge of lab techniques and instruments including electrophoresis, chromatography, microscopy, spectroscopy, PCR, Flow cytometery, protein assay, DNA isolation techniques, polymer synthesis and characterization, and synthetic fiber production Developed strong knowledge of FDA, GLP, GMP, GCP, and GDP regulatory requirements Created biocompatible photocurable hydrogels for cell immobilization Formulated cell friendly prepolymer formulation Performed surface modification of nano - particle fillers to enhance their biocompatibility Evaluated cell and biomaterial interaction, cell growth, and proliferation Designed bench - top experiments and protocols to simulate in vivo situations Designed hydrogel based microfluidic prototypes for cell entrapment and cell culture utilizing computer - aided robotic dispenser Determined various mechanical, morphological, and transport properties of photocured hydrogels using Instron, FTIR, EDX, X-ray diffraction, DSC, TGA, and DMA Assessed biocompatibility of hydrogels and physiology of entrapped cells Evaluated intracellular and extracellular reactions of entrapped cells on spatial and temporal scales using optical, confocal, fluorescence, atomic force, and scanning electron microscopies Designed various biochemical assays Developed thermosensitive PET membranes for transdermal drug delivery application using Gamma radiation induced graft co-polymerization of N - isopropyl acylamide and Acrylic acid Characterized grafted co-polymer using various polymer characterization techniques Manipulated lower critical solution temperature of grafted thermosensitive co-polymer Loaded antibiotic on grafted co-polymer and determined drug release profile with temperature Determined biomechanical and biochemical properties of biological gels isolated from marine organisms Analyzed morphological and mechanical properties of metal coated yarns using SEM and Instron Performed analytical work on pharmaceutical formulations using gas and high performance liquid chromatography Performed market research and analysis for medical textile company Developed and implement comprehensive marketing and sales campaign

Trace Determination of Tamoxifen in Biological Fluids Using Hollow Fiber Liquid - Phase Microextraction Followed by High - Performance Liquid Chromatography - Ultraviolet Detection

HbA1C was assessed using the high pressure liquid chromatography (HPLC) method.