Sentences with phrase «using restriction fragment»
The plasmids targeting each gene were respectively transfected into goat fibroblasts, and their genome modification efficiency was determined at day 3 using restriction fragment length polymorphism (RFLP) assay (see Methods).
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These studies were performed using Terminal Restriction Fragment analysis (TRFL - P), a technique that, although extremely useful for community profiling, has some intrinsic limitations.
By the 1990s, DNA testing had advanced somewhat through the use of restriction fragment length polymorphism, but that technique required more material than was available.