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Using calcium imaging on zebrafish, we were able to visualize an on - going process of memory consolidation for the first time.
Not exact matches
While powerful and widely
used,
calcium imaging alone is too slow to generate the kind of real - time map that the BAM project requires, says Michael Roukes of the California Institute of Technology in Pasadena.
To image the neurons firing, the researchers
used a technique called
calcium imaging that creates fluorescence in response to the influx of
calcium that occurs when a neuron fires.
Because
calcium imaging is widely
used to identify cells in tissues throughout the body, researchers studying other systems will be able to benefit from the published methodology.
To support their behavioral studies, the HHMI Janelia group performed deep brain
imaging in freely - moving AGRP - specific
calcium reporter mice
using miniature head - mounted fluorescent microscopes.
But
using a technique called
calcium imaging, Aoki et al. were able to visualize for the first time the activity of the whole zebrafish brain during memory retrieval.
The screening was conducted
using Cardiac Computed Tomography Angiography (CCTA), a noninvasive
imaging technology that accurately measures
calcium deposits and plaque buildup in the arteries.
Up until now, researchers who wanted to observe neuronal activity in real - time often
used a technique called
calcium imaging.
The researchers
used two - photon
calcium imaging and patch - clamp electrophysiology, two sophisticated technologies that allowed the researchers to record the signals from individual brain cells.
We will
use molecular, histological,
calcium imaging and electrophysiological methods to characterize the sensitivity of these channels to membrane stretch by mimicking the effect of IOP on RGC perikarya and axons.
In my current research, I investigate the sublethal effects of neonicotinoids on olfactory processing and learning
using in - vivo
calcium imaging.
Mapping of presynaptic nicotinic acetylcholine receptors
using fluorescence
imaging of neuritic
calcium.
The results from the
calcium imaging experiments
using brain explants and IPC - specific CCHa2 - R knockdown strongly suggest that CCHa2 crosses the blood - brain barrier (BBB) to regulate the IPCs, although the underlying mechanism remains elusive.
Using flourescence
imaging, the scientists tested
calcium response (an important nervous system ion implicated in the symptoms of bipolar disorder) in olfactory nerve cell samples from 17 bipolar patients (10 currently taking medication, 7 medication - free) with those of matched controls.
In his lab we
use multidisciplinary approaches that include behavioral experiments in the field and in the laboratory, neuroanatomy, in vivo electrophysiology,
calcium imaging and modeling.
He studied functional circuit processing throughout the visual system, including thalamus, primary visual cortex and extrastriate visual areas
using in vivo two - photon
imaging,
calcium sensors and viral tracers.
ACT - activated clotting time (bleeding disorders) ACTH - adrenocorticotropic hormone (adrenal gland function) Ag - antigen test for proteins specific to a disease causing organism or virus Alb - albumin (liver, kidney and intestinal disorders) Alk - Phos, ALP alkaline phosphatase (liver and adrenal disorders) Allergy Testing intradermal or blood antibody test for allergen hypersensitivity ALT - alanine aminotransferase (liver disorder) Amyl - amylase enzyme — non specific (pancreatitis) ANA - antinuclear antibody (systemic lupus erythematosus) Anaplasmosis Anaplasma spp. (tick - borne rickettsial disease) APTT - activated partial thromboplastin time (blood clotting ability) AST - aspartate aminotransferase (muscle and liver disorders) Band band cell — type of white blood cell Baso basophil — type of white blood cell Bile Acids digestive acids produced in the liver and stored in the gall bladder (liver function) Bili bilirubin (bile pigment responsible for jaundice from liver disease or RBC destruction) BP - blood pressure measurement BUN - blood urea nitrogen (kidney and liver function) Bx biopsy C & S aerobic / anaerobic bacterial culture and antibiotic sensitivity test (infection, drug selection) Ca +2
calcium ion — unbound
calcium (parathyroid gland function) CBC - complete blood count (all circulating cells) Chol cholesterol (liver, thyroid disorders) CK, CPK creatine [phospho] kinase (muscle disease, heart disease) Cl - chloride ion — unbound chloride (hydration, blood pH) CO2 - carbon dioxide (blood pH) Contrast Radiograph x-ray image
using injected radiopaque contrast media Cortisol hormone produced by the adrenal glands (adrenal gland function) Coomb's anti- red blood cell antibody test (immune - mediated hemolytic anemia) Crea creatinine (kidney function) CRT - capillary refill time (blood pressure, tissue perfusion) DTM - dermatophyte test medium (ringworm — dermatophytosis) EEG - electroencephalogram (brain function, epilepsy) Ehrlichia Ehrlichia spp. (tick - borne rickettsial disease) EKG, ECG - electrok [c] ardiogram (electrical heart activity, heart arryhthmia) Eos eosinophil — type of white blood cell Fecal, flotation, direct intestinal parasite exam FeLV Feline Leukemia Virus test FIA Feline Infectious Anemia: aka Feline Hemotrophic Mycoplasma, Haemobartonella felis test FIV Feline Immunodeficiency Virus test Fluorescein Stain fluorescein stain uptake of cornea (corneal ulceration) fT4, fT4ed, freeT4ed thyroxine hormone unbound by protein measured by equilibrium dialysis (thyroid function) GGT gamma - glutamyltranferase (liver disorders) Glob globulin (liver, immune system) Glu blood or urine glucose (diabetes mellitus) Gran granulocytes — subgroup of white blood cells Hb, Hgb hemoglobin — iron rich protein bound to red blood cells that carries oxygen (anemia, red cell mass) HCO3 - bicarbonate ion (blood pH) HCT, PCV, MHCT hematocrit, packed - cell volume, microhematocrit (hemoconcentration, dehydration, anemia) K + potassium ion — unbound potassium (kidney disorders, adrenal gland disorders) Lipa lipase enzyme — non specific (pancreatitis) LYME Borrelia spp. (tick - borne rickettsial disease) Lymph lymphocyte — type of white blood cell MCHC mean corpuscular hemoglobin concentration (anemia, iron deficiency) MCV mean corpuscular volume — average red cell size (anemia, iron deficiency) Mg +2 magnesium ion — unbound magnesium (diabetes, parathyroid function, malnutrition) MHCT, HCT, PCV microhematocrit, hematocrit, packed - cell volume (hemoconcentration, dehydration, anemia) MIC minimum inhibitory concentration — part of the C&S that determines antimicrobial selection Mono monocyte — type of white blood cell MRI magnetic resonance
imaging (advanced tissue
imaging) Na + sodium ion — unbound sodium (dehydration, adrenal gland disease) nRBC nucleated red blood cell — immature red blood cell (bone marrow damage, lead toxicity) PCV, HCT, MHCT packed - cell volume, hematocrit, microhematocrit (hemoconcentration, dehydration, anemia) PE physical examination pH urine pH (urinary tract infection, urolithiasis) Phos phosphorus (kidney disorders, ketoacidosis, parathyroid function) PLI pancreatic lipase immunoreactivity (pancreatitis) PLT platelet — cells involved in clotting (bleeding disorders) PT prothrombin time (bleeding disorders) PTH parathyroid hormone, parathormone (parathyroid function) Radiograph x-ray image RBC red blood cell count (anemia) REL Rocky Mountain Spotted Fever / Ehrlichia / Lyme combination test Retic reticulocyte — immature red blood cell (regenerative vs. non-regenerative anemia) RMSF Rocky Mountain Spotted Fever SAP serum alkaline phosphatase (liver disorders) Schirmer Tear Test tear production test (keratoconjunctivitis sicca — dry eye,) Seg segmented neutrophil — type of white blood cell USG Urine specific gravity (urine concentration, kidney function) spec cPL specific canine pancreatic lipase (pancreatitis)-- replaces the PLI test spec fPL specific feline pancreatic lipase (pancreatitis)-- replaces the PLI test T4 thyroxine hormone — total (thyroid gland function) TLI trypsin - like immunoreactivity (exocrine pancreatic insufficiency) TP total protein (hydration, liver disorders) TPR temperature / pulse / respirations (physical exam vital signs) Trig triglycerides (fat metabolism, liver disorders) TSH thyroid stimulating hormone (thyroid gland function) UA urinalysis (kidney function, urinary tract infection, diabetes) Urine Cortisol - Crea Ratio urine cortisol - creatine ratio (screening test for adrenal gland disease) Urine Protein - Crea Ratio urine protein - creatinine ratio (kidney disorders) VWF VonWillebrands factor (bleeding disorder) WBC white blood cell count (infection, inflammation, bone marrow suppression)
Using a materials science approach, the team tapped several high - tech
imaging methods to show that corals
use acid - rich proteins to build rock - hard skeletons made of
calcium carbonate minerals.