Sentences with phrase «using cells extracted»

She is one of 16 patients in the first U.S. trial using cells extracted from adult bone marrow to reverse advanced heart failure.

Not exact matches

Instead, the training is about using precise modern techniques to break up fights among inmates or to extract prisoners from cells, Garcia said, noting that guards frequently storm into those situations five or six at a time and begin using elbows, knees, batons, or pepper spray to force inmates into submission.

We extract the DNA from the cheek cells and use it to create a series of 32 numbers that are unique to that donor.

He also found cells of D. audaxviator, a bacterium that made up 99.9 % of the organisms he recovered from one of the filters used to extract water from rock fractures deep in the mines.

The researchers collected samples of indoor dust from 11 homes in North Carolina and tested extracts from the samples in a mouse pre-adipocyte cell model, 3T3 - L1 cells, often used to test compounds for potential effects on the accumulation of triglycerides, a type of fat.

Researchers... have extracted teichoic acid from the bacteria's cell wall and used it to protect groups of mice from subsequent massive doses of virulent staph organisms.

Lu's team will extract immune cells called T cells from the blood of the enrolled patients, and then use CRISPR — Cas9 technology — which pairs a molecular guide able to identify specific genetic sequences on a chromosome with an enzyme that can snip the chromosome at that spot — to knock out a gene in the cells.

We decided on a very basic goal: we would use the powerful editing tool to cut DNA he had already extracted from yeast cells.

Using a simple membrane extract from spinach leaves, researchers from the Technion - Israel Institute of Technology have developed a bio-photo-electro-chemical (BPEC) cell that produces electricity and hydrogen from water using sunlUsing a simple membrane extract from spinach leaves, researchers from the Technion - Israel Institute of Technology have developed a bio-photo-electro-chemical (BPEC) cell that produces electricity and hydrogen from water using sunlusing sunlight.

Designer Linda Loudermilk — a pioneer in environmental fashion whose collection is available at a wide range of boutiques — says she uses eco-friendly fabrics exclusively and adds to the list such exotic sustainable fibers as sea cell (a seaweed - based fiber), soy fiber * (extracted from the residue of soybeans used in the manufacture of tofu), and sasawashi (a linenlike blend of Japanese paper and kumazasa herb with antibacterial properties that «can heal a cut on your leg» — or so Loudermilk claims).

Nerve cells extracted from a patient's own nose could one day be used to cure paralysis.

sNuc - Seq bypassed those problems by using individual nuclei extracted from cells as a starting point instead.

Hang and coworkers exposed the human cells by first extracting the compounds from the paper with a culture medium then using the medium to culture the human cells for 24 hours.

We identified c - Jun amino - terminal kinase 3 (JNK3) as a binding partner of β - arrestin 2 using a yeast two - hybrid screen and by coimmunoprecipitation from mouse brain extracts or cotransfected COS - 7 cells.

ETH researchers have developed a method using a nanosyringe whose tiny needle is able to penetrate single living cells and extract their content.

• News from the World of Adult - Stem Cell Research • At the University of California at Los Angeles, Marc Hedrick's team used human adult fat cells extracted during liposuction to make cells resembling cartilage, bone, and muscle.

Using enzymes extracted from Escherichia coli bacteria, her team built an enzymatic fuel cell two years ago that runs on ethanol and can be attached directly to a circuit.

Benjaminson and his team have extracted stem cells from fish embryos and used them to grow muscle cells by stimulating them electrically, mechanically, hormonally and nutritionally.

Several methods can be employed to harvest it — for example, engineers can extract methane through anaerobic (oxygen - free) digestion, or produce electricity using microbial fuel cells.

Sadoway said a demonstration plant is still about three years away, but the systems would be analogous to cells used to extract aluminum.

The toxicity of chokeberry extract on normal blood vessel lining cells was tested and found to have no effects up to the highest levels used (50 ug / ml), suggesting that the cell death effect is happening in a way other than through preventing new blood vessel formation (anti-angiogenesis), a process that is important in cancer cell growth.

The study used a well - known line of pancreatic cancer cells (AsPC - 1) in the laboratory and assessed how well this grew when treated with either the chemotherapy drug gemcitabine or different levels of commercially available chokeberry extract alone, and when treated with a combination of gemcitabine and chokeberry extract.

Cells were extracted from the biopsy of her tumor sample for use in research without her knowledge or consent.

But many in the field will be waiting for the next steps: testing whether the resulting mouse pups are genetically normal, trying out the technique in other animals, and using other and less controversial types of cells — such as stem cells that can be extracted and cryopreserved from adult testicular tissue — as the starting point.

The researchers harvested satellite cells from both healthy and injured muscle tissue of young mice and from healthy tissue of old mice; extracted these cells» DNA with the histone coatings intact; and used tagged antibodies targeting the different kinds of marks to find which spots on those histones were flagged with either «stop» or «go» signals.

The cell - free DNA extracted from the plasma and, separately, the genome of white blood cells were then sequenced using the high - intensity, 508 - gene sequencing assay.

Barry Schonwetter and colleagues at the Magainin Research Institute, a private laboratory run by the drugs company Magainin Pharmaceuticals in Plymouth Meeting, Pennsylvania, took cells from the surface of cows» tongues and used organic solvents to extract several chemicals.

Using standard liposuction, a small volume of the patient's fat tissue (< 450 ml) was extracted and then the cells were separated from the tissue and concentrated (Celution ® System, Cytori Therapeutics, San Diego) on - site.

In the new research, Prins and Liau used a technique called adoptive cell transfer, which involves extracting and growing immune cells outside of the body, then reprogramming them with a gene known as New York Esophageal Squamous Cell Carcinoma, or NY - ESO cell transfer, which involves extracting and growing immune cells outside of the body, then reprogramming them with a gene known as New York Esophageal Squamous Cell Carcinoma, or NY - ESO Cell Carcinoma, or NY - ESO - 1.

«A very futuristic view is that maybe we will be able to extract energy from individual cells using similar designs,» she says.

Total RNA was extracted from frozen adipose tissue (100 mg), FACS - isolated cells (> 105), or cultured cells (60 - mm confluent plate) using a commercially available acid - phenol reagent (TRIzol; Invitrogen Corp.).

Similar protocols were used for immunoblot analysis of cell protein extracts from MC3T3 - E1 (mouse preosteoblasts), U-2 OS (human osteosarcoma), and C2C12 (mouse myoblasts with osteogenic potential).

In our JTM paper, work that was primarily supported by the Chronic Fatigue Initiative, we sequenced the mitochondrial DNA from a cohort of ME / CFS patients and healthy individuals, using DNA extracted from white blood cells stored in the biobank developed by the Chronic Fatigue Initiative.

After homogenizing these samples with a bead cell crusher (MS - 100; Tomy, Tokyo, Japan), total RNA was extracted using TRIzol reagent.

Back when stem cells were first extracted from human embryos 20 years ago, scientists were fascinated at their ability to change into any type of cell in the body and thought they would soon be used to treat all types of diseases, from eye disorders to diabetes.

Total RNA from Cellartis enhanced hiPS - HEP cells from C12, C18, and C22 (n = 2 batches per cell line) was extracted on Day 13 post-thawing as well as from hphep cells (n = 3 donors) Day 1 post-thawing using the GenElute RNA / DNA / Protein Plus Purification Kit (Sigma Aldrich).

Extracted cells are not full embryos, so embryonic stem cell researchers have been able to spend federal dollars provided the cells were extracted using nonfederExtracted cells are not full embryos, so embryonic stem cell researchers have been able to spend federal dollars provided the cells were extracted using nonfederextracted using nonfederal money.

Next, they extracted the DNA from developing and mature heart cells, using an advanced gene - sequencing technique called ChIP - seq that lets scientists «see» the epigenetic signatures written in the DNA.

The MS - D approach is already being used to extract biological structure from cell images, and is expected to provide a major new computational tool to analyze data across a wide range of research areas.

Protein concentrations in the cell extracts were determined using the BCA protein assay (Pierce supplied by Thermo Fisher Scientific, Rockford, IL, USA).

Protein was extracted into 200 μl of Luciferase Cell Culture Lysis Reagent (Promega), and 40 μl of protein extract was used for luciferase assays.

They presented frontline research in various fields such as large - scale methods to identify novel drug targets, how to extract more information from microscopic images by the use of computational methods, as well as the movement and behavior of immune cells.

We combine it with the use of human tissue culture cells in which we validate some of the results obtained in egg extract.

An in depth analysis of this phenomenon reveals that aggressive cancer cells are «parasites» that use oxidative stress as a «weapon» to extract nutrients from surrounding stromal cells.

For cell lines, early - split samples were used as duplicates and total RNA was extracted using the RNeasy mini kit.

In 2011 scientists demonstrated that stem cells extracted from a rat's brain could be made to produce insulin and used to cure diabetes in the same ratANCHOR.

The researchers also showed that their method can be used to determine the shape of the cell by injecting a dye; they are now working on extracting a cell's contents to read its genetic profile.

The extract was tested in laboratory studies using colon cancer cells grown in culture.

Nuclear extracts were prepared from H2122 cells using NE - PER Nuclear and Cytoplasmic Extraction Reagents (Pierce Biotechnology, Rockford, IL, USA).

RNA was extracted from different cell populations using Trizol ™ (Invitrogen) and precipitated with isopropanol.