Using cultured cells derived from human tumors of the breast and prostate gland, they confirmed that the IL6R / STAT3 / miR -34 a feedback loop is also activated in other tumor types.
Furthermore,
using cultured cells, they examined how the sex - related genes were regulated.
Years ago W.U.'s Holtzman and former colleague Marc Diamond, who has since moved to the University of Texas Southwestern Medical Center, identified tau - binding antibodies that slow the spread of toxic tau clusters in a lab assay
using cultured cells.
Exactly how the genetic risk factors contribute to the disease is not readily apparent in most cases, and requires cellular and molecular biology investigations that
use cultured cells and mouse models.
Not exact matches
One habit of Prince's they told me about struck me as so counter to our times that I came to see it as illustrating what set him apart from today's
culture of celebrity: he allowed no one near him to
use a
cell phone.
Bethencourt and Shigeta figured they could
use cell cultures to grow meat without slaughtering any cows or chickens — but why rely on those animals at all?
In my
cell biology course, we investigate the biology and chemistry of a
cell surface receptor that helps induce good feelings in us when it binds to a chemical compound found in incense; this may help explain why so many different
cultures and religions have independently evolved the
use of incense in their ceremonies and rituals.
While some still object to
cells being taken from animals and
used by scientists to grow clean meat in laboratories — and some just don't like the idea of eating a «
cultured» steak created by men and women in white coats — others see the lab - grown meat revolution as key to solving the environmental crisis linked to meat eating.
3M Delivers Early Purification with New Hybrid Purifiers, Single -
use functionalised media provides scalable purification performance in
cell culture
This allowed them to track changes in calcium ion concentrations in the
cells around wounds in living tissue (as opposed to the
cell cultures used in many previous wound response studies) and to do so with an unprecedented, millisecond precision.
While other papers have examined these mutations
using expensive and time - consuming experiments on live ferrets and laboratory
cell cultures, Deem and Melia Bonomo
used the pEpitope method to rapidly calculate how much the egg - passage mutations would decrease vaccine efficacy in humans.
Shukla and colleagues discovered that a small drug molecule called BX795, which is sold to labs for
use in experiments, helped clear HSV - 1 infection in
cultured human corneal
cells, in donated human corneas, and in the corneas of mice infected with HSV - 1.
Researchers from Duke University had previously
used CRISPR to correct genetic mutations in
cultured cells from Duchenne patients, and other labs had corrected genes in single -
cell embryos in a laboratory environment.
In the journal Biomaterials, the team reports
culturing cells to make extracellular matrix (ECM) of two types and five different alignments with the strength found in natural tissue and without
using any artificial chemicals that could make it incompatible to implant.
This study identifies myomerger as a fundmentally required protein for muscle development
using cell culture and laboratory mouse models.
Bioengineers at the University of California, San Diego have proven that when it comes to guiding stem
cells into a specific
cell type, the stiffness of the extracellular matrix
used to
culture them really does matter.
Despite being trapped and baked within a polymer,
cell staining shows that the
cultured cells remained alive, says Fernandez, an obvious necessity if they are to be
used for artificial tissues.
Researchers then tested
cell cultures and mouse models by
using a gene editing process called CRISPR - Cas9 to demonstrate how the presence or absence of myomaker and myomerger — both individually and in unison — affect
cell fusion and muscle formation.
Protein expression in these glioblastoma
cells more closely mimicked that in real cancer
cells than in 2D
cultures of
cells, indicating that this method could be
used to study cancer (Nature Nanotechnology, DOI: 10.1038 / nnano.2010.23).
Using human fetal «mini-brains» grown in 3 - D
cultures, scientists determined that a specific protein produced by the Zika virus changes the properties of neural stem
cells in the developing brain of an infected fetus, potentially causing microcephaly in newborns (Ki - Jun Yoon, abstract 103.06, see attached summary).
«If we win, the entire regulatory structure for autologous
cell processing, with or without
culture, will be rewritten such that any physician
using good practices and treating patients responsibly can
use stem
cells as part of his or her medical practice,» he says.
Dr. Zubair already grows such
cells in his Mayo Clinic laboratory
using a large tissue
culture and several incubators — but only at a snail's pace.
They then
used fluorescent antibodies that bind specifically to the protein to trace the virus's interactions with various kinds of
cultured cells.
The
cells used to build the organs could be
cultured from stem
cells taken from fat or bone - marrow tissue, he says: «We could engineer a blood vessel from your own
cells.»
Although the bulk of the commercial manufacturing
uses cultures of bacteria, such as Escherichia coli or Chinese hamster ovary
cells, a few biotech companies are trying to produce therapeutic proteins in the milk of transgenic mammals (such as GTC Biotherapeutics, which is
using goats; PPL Therapeutics, which is
using sheep; and BioProtein Technologies, which is working with rabbits), transgenic chicken eggs (such as Avigenics or Vivalis), or even in transgenic crops (such as ProdiGene or Meristem Therapeutics); but it is early days for these «pharming» methods.
Droplet - based microfluidics is a tool
used in various applications like
cell cultures, chemical synthesis and DNA sequencing.
Often times we'll
use an animal model or
cell culture model to test a bioactive to see if it has efficacy.
The students
used a 3 - D printer to design a smaller and more cost - efficient bioreactor — a miniaturized
culture device where neural stems
cells are grown and eventually become small laboratory brains for research.
The team
used human embryonic stem
cells — which can transform into any
cell of the body — and
cultured them in a mixture of chemicals to grow human brain
cells.
The study team removed fibroblasts (skin
cells) from DBA patients, and in
cell cultures,
using proteins called transcription factors, reprogrammed the
cells into iPSCs.
When we first posted this story, we
used the headline: «Stem
cell scientists reveal «unethical» work
culture».
Currently, most influenza vaccines in the United States are produced
using chicken eggs, while a few are made in
cell culture or by
using recombinant DNA technologies.
However, this coupling does not exist in
cell culture models with exogenously expressed c - MYC — the type of systems that are mainly
used to investigate glutamine addiction in cancer.
The researchers then
used a 3 - D
cell culture system to coax their mutant and healthy hPSCs down the differentiation pathway into becoming kidney progenitor
cells and finally the proximal tubule
cells found in kidney nephrons.
Writing in the May 4 online issue of the journal Scientific Reports, researchers at University of California, San Diego School of Medicine
used a powerful statistical tool called «design of experiments» or DOE to determine the optimal
cell culture formula to grow and produce hPSCs.
Cellular toxicity was unrelated to the type of «carrier» solution
used in the
cell cultures, the
use of HES made from different origins (potato versus corn starch), or the time
cells spent in
culture with HES.
Despite a lack of rigorous clinical trials to test safety or efficacy, a ban of the treatment by health authorities, and a lack of peer review by the scientific community, the privately funded Stamina Foundation has been
using cultured mesenchymal stem
cells that have been exposed to putative conditions that favour neuronal differentiation to treat different diseases in severely or terminally ill patients.
Human chondrosarcoma HCS - 2 / 8
cells, which are a type of benign bone cancer
cells, can be
used to investigate how optimization of
culture conditions could improve the synthesis of cartilage - specific molecules.»
The new research, which studied the immortalization process
using genome - engineered
cells in
culture and also tracked skin
cells as they progressed from a mole into a malignant melanoma, suggests that telomerase plays a more complex role in cancer.
They might be put to
use right away to track
cultures of immune
cells as they migrate in response to chemical stimuli.
The
cells will enable researchers to
use lab - grown
cultures to study how the diseases develop.
«What made the difference are our unique
cell culture conditions and 3 - D matrix, which allowed
cells to grow and develop much faster and longer than the 2 - D
culture approaches that are more typically
used.»
Using a labor - intensive
cell culturing technique for hunting retroviruses that he had pioneered in the 1970s, Ruscetti had transmitted the pathogen from patients» T -
cells to uninfected T -
cells in the laboratory.
To unravel the complex journey of the body's GPCRs, scientists
used biophysical tools, including fluorescent biosensors; biochemical tools, such as
cell signaling in neuronal
cultures; as well as computational models.
Hsiao and colleagues carefully gathered skin samples from donors, and then grew the skin
cells in
culture before converting them into iPS
cells using the methods created by Yamanaka.
We were able to
use pharmaceuticals to induce
cell self - eating effects and stimulate dedifferentiation though not as effectively as 3D
culture, so we need to do more work on this.
After the pig experiment worked well, he
used his Boston area facility to
culture the 30 million cardiac muscle
cells that he says were injected into a heart disease patient in mid-2011.
Cultured human
cells are a better alternative and are already
used by one manufacturer, says the report.
In animal and
cell culture studies, the drug inhibited growth both in estrogen - dependent breast cancer
cells and in
cells that had developed resistance to the anti-estrogen tamoxifen and / or to the aromatase inhibitors, two of the most widely
used types of drugs to prevent and treat estrogen - dependent breast cancer.
The journal's home page explains that translational medicine «builds on basic research advances — studies of biological processes
using cell cultures, for example, or animal models — and
uses them to develop new therapies or medical procedures.»