Sentences with phrase «using electroporation»

Using electroporation — in Corn's words, «we run an electric current and make holes in the cells» — he'll inject the complex and replacement parts through the cells» protective membrane.

Typically, Addgene uses electrocompetent Stbl4 cells when performing amplifications, and we use electroporation to ensure a good transformation efficiency.

Not exact matches

Using Pulse Electric Field Processing (PEF) the patent protected E-FLO Electroporation System provides significant product improvement in chip crunch, taste and texture as well as reduction in acrylamide formation and oil content.

«New innovative method for delivering genes into cells: Modification of cell physiological function with novel parallelized electroporation of mammalian cells by using electrostatic manipulation in a water - in - oil droplet.»

The first authors, Assistant Professor Hirofumi Kurita and Professor Akio Mizuno, said, «W / O droplet electroporation is operated using a DC power supply, which obviates the need for an expensive pulse generator.

However, most commercial electroporation - based transfection methods require the use of specialized pulse generators to produce short electrical pulses at high voltage.

There have been improvements in W / O - droplet electroporation electrodes for use in disposable 96 - well plates for concurrent performance (Fig. 2).

In addition, the size of the droplet is quite small compared with that in conventional bulk electroporation, resulting in the use of fewer materials in high - throughput analysis.»

They then used a method known as electroporation, in which cells are briefly exposed to an electrical field that makes their membranes more permeable, to quickly deliver these RNPs to the interior of the cells.

For both BayGenomics parental ES cell line E14Tg2a.4 (also used for Sanger (SIGTR) lines) and Soriano parental ES cell line AK7, ultra low passage cells suitable for multiple electroporations and germline transmission are available.

INO - 3112 is injected into a muscle followed by electroporation, a technique that uses controlled, millisecond electrical pulses that make it easier for the DNA plasmids to enter muscle cells.

Whether a protocol uses cell culture methods to expand edited cells for downstream assays, to increase the efficiency of lentiviral transduction or to let cells «rest» after electroporation, optimized culture conditions are key to maintaining cell viability and biological function.

Cell culture steps were used by both groups to let cells recover after thawing and / or electroporation, and additionally to generate erythroid progenitor cells from edited CD34 + cells [1].

Electroporation of cultured cells was carried out using the Neon Transfection System (Invitrogen).

For a typical library, Addgene usually does four to eight electroporation reactions using a total of ~ 400ng of library DNA.

In this study we review the most commonly used indel detection methods and using a robust, sensitive, and cost efficient Indel Detection by Amplicon Analysis method, we have investigated the impact of the most commonly used CRISPR / Cas9 delivery formats, including lentivirus transduction, plasmid lipofection, and ribo nuclear protein electroporation, on the dynamics of indel profile formation.

Additionally, the authors tested for cellular specificity by using as a convenient experimental framework in utero electroporation of a plasmid that allowed co-expression of the PLE enzyme and of a fluorescent tag (mCherry).