Sentences with phrase «using endogenous proteins»

Mai said that «in the future, we plan to combine our tool with genome editing, which we will use to specifically insert sequence encoding CIB1 into the genome, enabling us to optogenetically control the intracellular membrane using endogenous proteins.

Not exact matches

Cell lines express endogenous or recombinant proteins and are available in a ready - to - use format to facilitate your research efforts

Using recombinant protein expression we can produce milligram quantities of recombinant convertases, as well as of their two endogenous binding proteins.

Targeted DNA demethylation and activation of endogenous genes using programmable TALE - TET1 fusion proteins.

Efforts to tag endogenous genes for proteins in these compartments using CRISPR systems should strongly consider using moxFPs over standard FPs for the reasons listed above.

To accomplish this, we used homologous recombination to ablate nonmuscle myosin heavy chain (NMHC) II - B by inserting cDNA encoding green fluorescent protein (GFP)- NMHC II - A into the first coding exon of the Myh10 gene, thereby placing GFP - NMHC II - A under control of the endogenous II - B promoter.

The use of endogenous HMOX1 protein expression as a readout allowed the identification of compounds that specifically derepress Bach1 and induce transcription of an Nrf2 - responsive gene.

Under conditions of starvation, endogenous sources (eg, muscle protein, glycogen, and fat stores) are used as energy supplies (10).

Adhering to these traditional concepts the US Department of Agriculture has concluded that diets, which reduce calories, will result in effective weight loss independent of the macronutrient composition, which is considered less important, even irrelevant.14 In contrast with these views, the majority of ad - libitum studies demonstrate that subjects who follow a low - carbohydrate diet lose more weight during the first 3 — 6 months compared with those who follow balanced diets.15, 16, 17 One hypothesis is that the use of energy from proteins in VLCKD is an «expensive» process for the body and so can lead to a «waste of calories», and therefore increased weight loss compared with other «less - expensive» diets.13, 18, 19 The average human body requires 60 — 65 g of glucose per day, and during the first phase of a diet very low in carbohydrates this is partially (16 %) obtained from glycerol, with the major part derived via gluconeogenesis from proteins of either dietary or tissue origin.12 The energy cost of gluconeogenesis has been confirmed in several studies7 and it has been calculated at ∼ 400 — 600 Kcal / day (due to both endogenous and food source proteins.18 Despite this, there is no direct experimental evidence to support this intriguing hypothesis; on the contrary, a recent study reported that there were no changes in resting energy expenditure after a VLCKD.20 A simpler, perhaps more likely, explanation for improved weight loss is a possible appetite - suppressant action of ketosis.