Sentences with phrase «using expression vector»

Clade A recombinant nef - protein, expressed as His - tag fusion protein in E. Coli using expression vector pET24 and purified using Ni - agarose columns

Recombinant RT protein from SIVmac clone J5 protein is expressed as His tag - fusion protein in E.coli using expression vector pET24.

Recombinant SIVmac Clone J5 rev protein is expressed as His tag - fusion protein in E.coli using expression vector pET24.

Clade C recombinant nef protein, expressed as His - tag fusion protein in E. coli using expression vector pET24 and purified using Ni - agarose columns.

Recombinant TAT protein is expressed as His tag - fusion protein in E.coli using expression vector pET24.

Clade B recombinant p17 / 24 - B protein is expressed as His - tag fusion protein in E.coli using expression vector pET24 and purified using Talon Superflow metal affinity resin.

Clade C recombinant p17 / 24 - B protein, expressed as His - tag fusion protein in E.coli using expression vector pET24 and purified using Talon Superflow metal affinity resin.

Clade B recombinant Nef protein, expressed as a His - tag fusion protein in E. coli using expression vector pET24 and purified using Ni - agarose columns.

Not exact matches

Using support vector machine recursive feature elimination, the researchers identified three gene expression signatures that predicted therapy responses.

May be used for the affinity purification of the SIV - Pk fusion protein from bacterial expression vectors 282 and 283.

The rev cDNA from pCV - 1 (NIH Cat # 303) was amplified using PCR and cloned into the expression vector pOTS Nco1 (SmithKline Beecham).

High level erythroid lineage - directed gene expression using globin gene regulatory elements following lentiviral vector mediated gene transfer into primitive human and murine hematopoietic cells.

Approaches such as the baculovirus expression vector system (BEVS) in use at uniQure (Amsterdam, The Netherlands) for manufacturing alipogene tiparvovec may provide suitable options (4).

AAV gene therapy vectors are emerging as the gene transfer vehicle with high potential for use in the CNS as they transduce post mitotic cells that mediate the sustained, long term gene expression required in chronic progressive diseases.

Striatal modulation of BDNF expression using microRNA124a - expressing lentiviral vectors impairs ethanol - induced conditioned - place preference and voluntary alcohol consumption.

Selected Amrose variant fluorescent proteins were subcloned into the pCS2 + expression vector [32](EcoRI - XbaI) using the gel purified amplicons generated above.

This expression construct was inserted into the pENTR vector of Invitrogen's Gateway system using the D - TOPO cloning kit.

Such therapies are based on using either adenoassociated virus (AAV) or lentivirus (1), both vectors being frequently generated through transient expression...

Including Algebraic Expressions, Writing Expressions using Diagrams, Algebraic Fractions, Equations, Formulae,, Functions, Graphical Functions, Inequalities, Linear Graphs, Proof, Quadratics,, Sequences, Simultaneous Equations and Vectors

Matsuo used the score as a vector for musical expression and felt relatively unrestrained by style, form, texture, and the expectations of listeners.

This is further reinforced by the references to vector graphics and geometrical primitives in Ashcroft's work, graphics based on mathematical expressions which are used to communicate non-subjective, procedural routes into abstraction at odds with the historical overtly - subjective precedents of Abstract Expressionism.»

That's why I used the expression «represents a serious potential threat to humanity and our environment» (temperature increase by 2100 of up to 6.4 C, increased droughts, floods, tropical cyclones, heat waves, extreme high sea level plus secondary effects, such as crop failures, spread of vector diseases, loss of drinking water from melting glaciers, etc. all as listed in IPCC AR4).