Sentences with phrase «using following»

If you're putting together your own workout, you can try the exercises listed above using the following training guidelines:

Please feel free to opt - in using the following link: https://www.research.net/r/wellness-signup We also provide the BIOHM Wellness Phone Consultation, which is a paid, one - on - one, phone consultation with a member of our BIOHM Wellness Team.

Freshen your home and protect against more serious problems like sick building syndrome (which we recently learned can be incredibly debilitating through a first - person account by Alan Bell) using the following techniques:

I also highly suggest using the following three detox support tools to strengthen digestion and boost the immune system throughout the year:

You can assess the level of toxic buildup in your body using the following five signs culled from Ayurvedic tradition:

If you've tried making mashed cauliflower potatoes before and didn't love the result, don't be afraid to try again using the following tips:

Each morning I sit in my chair, take a couple of sips of coffee, and begin Step 1 — clearing reactivity — by resetting my vagus nerve using the following sequence to help loosen up and release tension all around.

Score yourself against the statements below using the following scale: 1 Never, 2 Rarely, 3 Sometimes, 4 Often, and 5 Always

When using the following affirmations, sit or lie down in a quiet place and focus on the location of each chakra.

These high - quality reads were then mapped to the corresponding XMRV reference genome using the following parameters (no gaps allowed, maximum mismatches allowed per read of 5 %, and maximum ambiguity of 1).

Authors who use data from the project must acknowledge DECIPHER using the following wording «This study makes use of data generated by the DECIPHER Consortium.

MUTAGENETIX should be cited in journal articles or on - line publications using the following conventions: -LCB- Authors, Science Writers, Beutler B -RCB-.

If Broad Institute contributes to the results being published, the authors must acknowledge Broad Institute using the following wording: «This study makes use of data shared through the Broad Institute matchbox repository.

Neurite length was calculated using the following formula; NL = α × T × (π / 2), where α is the number of times the neurite intersects the grid lines and T is the distance between the gridlines on the magnified image (taking into account the magnification factor) or was calculated using ImageJ analysis software.

After both quality control steps, multi-sample SNP / genotype calling was carried out using the algorithm implemented in Samtools [65] using the following criteria.

From selected areas, immunohistochemical reactions were performed using the following antibodies and dilutions: anti — glial fibrillary acidic protein (1:500), anti-neurofilament (1:2000), anti-CD3 (1:1000), anti-CD20 (1:1000), and anti-CD68 (1:1000).

Phosphorylation of signaling proteins was investigated using the following phospho - specific antibodies from Cell Signaling Technology: anti-pY1034 / 1035 JAK1 (# 3331S), anti-pY1007 / 1008 JAK2 (# 3771S), anti-pY705 STAT3 (# 9131S), anti-pY694 STAT5 (# 9351S) and anti-p44 / 42 MAPK (# 9101L).

The ARM Climate Research Facility should be acknowledged in publications as the origin of field studies or data used in the research using the following guidelines.

After 10 minutes on ice, the samples were sonicated using the following protocol: 2 × 30 seconds at 30 % power, 2 × 30 seconds at 35 % power, 2 × 30 seconds at 40 % power, 2 × 30 seconds at 45 % power.

The hybridization and processing of the GeneChips were conducted by Salk Institute's Functional Genomics Core Facility using the following systems from Affymetrix (Santa Clara, CA, USA): GeneChip ® Hybridization Oven 640, GeneChip ® Fluidics Station 450 to the wash and stain operation of Affymetrix GeneChip ® arrays, and the GeneChip ® Scanner 3000 7G.

Proposals can be submitted using the following web form through October 16, 2017.

The parameters were set to screen the matches using the following conditions: (1) identity of ≥ 95 %, (2) coverage of ≥ 98 %, and (3) no insertions or deletions.

Hematopoietic malignancies were diagnosed using the following markers: for B - and T - lymphocyte detection, rat antimouse CD45R / B220 mAb (Southern Biotechnology Associates, Birmingham, AL; dilution 1:100); for T - lymphocyte detection, rabbit antihuman CD3 polyclonal antibody (Dako, Glostrup, Denmark; 1:100); and for monocyte / macrophage detection, rat antimouse F4 / 80 mAb (BMA Biomedicals AG, Augst, Switzerland; 1:20) and rat antimouse CD11b mAb (Chemicon International, Temecula, CA; 1:20).

The coding sequence of chicken Acvr1 was PCR amplified from chicken embryo cDNA using the following primer pair: chAcvr1 - NcoI - fwd, 5 ′ - ACCATGGCTCTCCCCGTGCTGCTG - 3 ′ and chAcvr1 - BamHI - rev, 5 ′ - AGGATCCTCAACAGTCAGCCTTCAGTTT - 3 ′.

Quantitative reverse transcriptase PCR (qRT - PCR) was conducted using the SYBR Green qPCR Kit (Fermentas) by using following primers: Actin F: TGAGACCTTCAACACCCCAGCCATG, Actin R: CGTAGATGGGCACAGTGTGGGTG, KLF4 F: GTCTTGAGGAAGTGCTGAGC, KLF4 R: ATCGTCTTCCCCTCTTTGGC.

This construct was used to introduce the corresponding human FOP mutation R206H and the constitutive active variant of the receptor Q207D by Site - Directed Mutagenesis (QuikChange, Stratagene) using the following primer pairs (with lower - case letters indicating the nucleotides changed relative to wild - type Acvr1 sequence): R206H - chAcvr1 - fwd, 5 ′ - GCAAAGAACAGTGGCTCaCCAGATCACGCTTGTGG - 3 ′ and R206H - chAcvr1 - rev, 5 ′ - CCACAAGCGTGATCTGGtGAGCCACTGTTCTTTGC - 3 ′; chAcvr1 - ca - Q207D - fwd, 5 ′ - GCAAAGAACAGTGGCTCGCgAcATCACGCTTGTGGAGTG - 3 ′ and chAcvr1 - ca - Q207D - rev, 5 ′ - CACTCCACAAGCGTGATgTcGCGAGCCACTGTTCTTTGC - 3 ′).

For each condition, 200 ng genomic DNA was then PCR amplified using Platinum Taq High Fidelity (Invitrogen) using the following primers plus 454 adaptor sequences and 8 letter DNA barcodes: CAACCTCTACAGCAGTGTCCTCATC (forward) and GGAGTGTGACAGCTTGGAGATG (reverse).

In addition, for this study, they created an autistic behavior section, using the following criteria: 1) whether reliability and validity data were available in typically developing individuals and / or in those with neurodevelopmental disorders; 2) whether the measure had been piloted in FXS clinical trials; and 3) whether the measure addressed an important aspect of the FXS symptom.

Since 1994, the United Nations General Assembly has repeatedly condemned terrorist acts using the following political description of terrorism:

Finally, the subjective health of the respondents was measured using the following question: «In general, how would you assess your health?»

Using the following are strategies can help you nurture good sleeping habits in your baby or toddler right from the start.

First, you can browse through the list of treating professionals on this website using the following link: http://www.selectivemutism.org/find-help/find-a-treating-professional/.

In order to provide employees and patrons with a safe and secure environment in which to work and play, the District reserves the right to close a particular facility or cancel a program using the following criteria:

Or give him ideas using the following suggestions to boost physical development:

Models were developed using the following possible predictors of breastfeeding duration: maternal race, maternal education, paternal education, maternal age, socioeconomic status, 22 marital status, parity, mode of delivery, previous breastfeeding experience, timing of feeding method selection, problems with pregnancy / labor / delivery, breastfeeding goal (weeks), family preference for breastfeeding, paternal preference for breastfeeding, having friends who breastfed, randomization group, 16 plans to return to work, infant's 5 - minute Apgar score, and infant's age in minutes when first breastfed (first successful latch and feeding).

Using the following go - to guide and very yummy spice tosses may change your mind this year while carving out that pumpkin — Enjoy!

Unfortunately you'll need to use some elbow grease to make them useful again using the following steps.

For 2000 - 2013, cause of death is determined using the following ICD - 10 codes: SIDS (R95), unknown cause (R99) and ASSB (W75).

This article provides the foundation you need to be successful using the following activity for setting goals with your children.

And you can join the conversation about this pump on Twitter using the following hashtag: #evenflocomfort

You can capitalize on this natural trend by using the following tips for teaching responsibility, to help your tween now and throughout his or her life.

If none of the remedies listed above are working for you, then it may be a good idea to contact your midwife or OB / GYN and talk to them about using the following remedies:

Using the following money saving tips, you can keep more money in your bank account and make more efficient use of the money you do spend.

Mountain biking is exhilarating and offers plenty of exercise, but not all kids are born to ride; rather, you must prepare them using the following tips.

«I made my own using the following directions - and was able to get flannel sheets to match my nursery decor for much less than the cost of manufactured ones.»

Tweet this giveaway using the following link (w / o quotation marks).

A: Please purchase using the following link:

Using the following ideas may give you the tools you need to deal with temper tantrums effectively and have a better behaved child, too.

Help your picky eater baby overcome the hesitation by using the following tips.

However, through out the Spring Series season Yeovil Town Ladies FC will also be using the following venues to play their home matches at: