Sentences with phrase «using following primers»

Quantitative reverse transcriptase PCR (qRT - PCR) was conducted using the SYBR Green qPCR Kit (Fermentas) by using following primers: Actin F: TGAGACCTTCAACACCCCAGCCATG, Actin R: CGTAGATGGGCACAGTGTGGGTG, KLF4 F: GTCTTGAGGAAGTGCTGAGC, KLF4 R: ATCGTCTTCCCCTCTTTGGC.

For each condition, 200 ng genomic DNA was then PCR amplified using Platinum Taq High Fidelity (Invitrogen) using the following primers plus 454 adaptor sequences and 8 letter DNA barcodes: CAACCTCTACAGCAGTGTCCTCATC (forward) and GGAGTGTGACAGCTTGGAGATG (reverse).

This construct was used to introduce the corresponding human FOP mutation R206H and the constitutive active variant of the receptor Q207D by Site - Directed Mutagenesis (QuikChange, Stratagene) using the following primer pairs (with lower - case letters indicating the nucleotides changed relative to wild - type Acvr1 sequence): R206H - chAcvr1 - fwd, 5 ′ - GCAAAGAACAGTGGCTCaCCAGATCACGCTTGTGG - 3 ′ and R206H - chAcvr1 - rev, 5 ′ - CCACAAGCGTGATCTGGtGAGCCACTGTTCTTTGC - 3 ′; chAcvr1 - ca - Q207D - fwd, 5 ′ - GCAAAGAACAGTGGCTCGCgAcATCACGCTTGTGGAGTG - 3 ′ and chAcvr1 - ca - Q207D - rev, 5 ′ - CACTCCACAAGCGTGATgTcGCGAGCCACTGTTCTTTGC - 3 ′).

The coding sequence of chicken Acvr1 was PCR amplified from chicken embryo cDNA using the following primer pair: chAcvr1 - NcoI - fwd, 5 ′ - ACCATGGCTCTCCCCGTGCTGCTG - 3 ′ and chAcvr1 - BamHI - rev, 5 ′ - AGGATCCTCAACAGTCAGCCTTCAGTTT - 3 ′.

Not exact matches

The following primers were used for sqPCR: TEKF: 5 ′ - CCAGGATGGCAGGGGCTCCA - 3 ′, TEKR: 5 ′ - GGTAGCGGCCAGCCAGAAGC - 3 ′; PECAM1F: 5 ′ - GTGAAGGTGCATGGCGTATC - 3 ′, PECAM1R: 5 ′ - CACAAAGTTCTCGTTGGAGGT - 3 ′; CDH5F: 5 ′ - CCTTCTTCACCCAGACCAAG - 3 ′, CDH5R: 5 ′ - GTGAAAGCGTCCTGGTAGTC - 3 ′; CSPG4F: 5 ′ - CCTGGAGAATGGTGGAAGAG - 3 ′, CSPG4R: 5 ′ - GGCCTGTGTTTGTAGTGAGGA - 3 ′; CD248F: 5 ′ - TCTGCCTGTGCTACCTTCTG - 3 ′, CD248R: 5 ′ - GCCTTTCTTGTCACCTCTGG - 3 ′; GAPDHF: 5 ′ - TGTCTCCTGCGACTTCAACA - 3 ′, GAPDHR: 5 ′ - GCCATGTAGGCCATGAGGT - 3 ′.

Primers used to amplify the human Snail gene have the following sequences: 5» - CCTCCCTGTCAGATGAGGAC - 3» (forward) and 5» - CCAGGCTGAGGTATTCCTG - 3» (reverse).

Primers used to amplify the human Slug gene have the following sequences: 5» - GGGGAGAAGCCTTTTTCTTG - 3» (forward) and 5» - TCCTCATGTTTGTGCAGGAG - 3» (reverse).

Primers used to amplify the human Twist gene have the following sequences: 5» - GGAGTCCGCAGTCTTACGAG - 3» (forward) and 5» - TCTGGAGGACCTGGTAGAGG - 3» (reverse).

The Quantitect One - Step RT - PCR kit (Qiagen, Hilden, Germany) was used with a 25 μl reaction mixture under the following conditions: 0.25 μl of kit enzyme mixture (including reverse transcriptase RT and Taq polymerase), 10 μl of 2 × Quantitect RT - PCR buffer, 1.25 μl of 10 μM of each primer, 0.5 μl of 10 μM of probe at 10 μM, 6.8 μl of DNA RNA free water (Mol Bio grade, Hamburg, Germany) and 5 μl of the extracted sample.

The primers used were as follows: AXL forward, 5 ′ - CAGCCTCCTCCTCACAGACA; AXL reverse, 5 ′ - GAGCCCTGATCATTCCACTG; AJUBA forward, 5 ′ - AAGGAAAGAGTGTGGGGGTAGG; AJUBA reverse, 5 ′ - ACGCTGGGAACAAAGTCACG; AMOTL2 forward, 5 ′ - TGCCAGGAATGTGAGAGTTTC; AMOTL2 reverse, 5 ′ - AGGAGGGAGCGGGAGAAG; WTIP forward, 5 ′ - GCAGCGCCGTCTCCTTCCT; WTIP reverse, 5 ′ - GCGGCGGAGGAATGTAAGCTC.

The primers used were as follows: mouse RANKL, 5 ′ - TTGCACACCTCACCATCA (forward) and 5 ′ - TACGCTTCCCGATGTTTC (reverse); mouse OPG, 5 ′ - CACTCGAACCTCACCACA (forward) and 5 ′ - CAAGTGCTTGAGGGCATA (reverse); mouse GAPDH, 5 ′ - CTCATGACCACAGTCCATGC (forward) and 5 ′ - ACACATTGGGGGTAGGAACA (reverse); human ABL1, 5 ′ - GGCTGTGAGTACCTTGCTGC (forward) and 5 ′ - GGCGCTCATCTTCATTCAGGC (reverse); human ABL2, 5 ′ - CCAGCTACTCCCGAGGCTG (forward) and 5 ′ - CTTGATCCCACAGGGTGAAG (reverse); human GAPDH, 5 ′ - GGCTCTCCAGAACATCATCCC (forward) and 5 ′ - GGGTGTCGCTGTTGAAGTCAG (reverse); human MMP1, 5 ′ - GGTCTCTGAGGGTCAAGCAG (forward) and 5 ′ - AGTTCATGAGCAACACG (reverse); human IL6, 5 ′ - AGACAGCCACTCACCTCTTC (forward) and 5 ′ - TTTCACCAGGCAAGTCTCCT (reverse); human TNC, 5 ′ - CCCTACGGGTTCACAGTTTC (forward) and 5 ′ - TTCCGGTTCGGCTTCTGTAAC (reverse); human AXL, 5 ′ - ATCAGACCTTCGTGTCCCAG (forward) and 5 ′ - ATGTCTTGTTCAGCCCTGGA (reverse); human WWTR1, 5 ′ - GGCTGGGAGATGACCTTCAC (forward) and 5 ′ - AGGCACTGGTGTGGAACTGAC (reverse); human YAP1, 5 ′ - ATGAACTCGGCTTCAGCCAT (forward) and 5 ′ - ACCATCCTGCTCCAGTGTTG (reverse).

Following lysis and solid - phase capturing of mRNAs with streptavidin magnetic beads, samples were reverse transcribed using random primers.

The following primer sequences were used:

The resulting first strand cDNA products were poly G - tailed followed by 30 rounds of PCR amplification using a single primer.

The primer sequences used were as follows:

I also use copaiba balsam instead of tea tree oil — it's gentler on the skin with just as much anti-inflammatory + anti-bacterial action, plus it has lots of anti-aging properties... for the ladies, broccoli seed oil is also a must have in their serum... natural silicones so excellent as a makeup primer plus it's very anti-inflammatory... Lastly, tamanu to heal scars from past breakouts... I also use the following essential oils: neroli, helichyrsum, and german chamomile.

I use the Mattifying Primer and this foundation, which just happens to be the perfect shade, followed by Bamboo Blur Powder.

The products that I have used in this look are as follows: Bare Minerals Prime Time Primer, MAC Pro-Longwear Foundation, Rimmel London Wake Me Up concealer, Rimmel London Hide The Blemish concealer, MAC Prep + Prime Highlighter, MAC Bronzing Powder, NARS Orgasm blusher, NARS Albatross Highlighting powder, MAC eyebrow pencil in brunette, Bare Miners Speak Your Mind Lipstick, Tanya Burr Afternoon Tea Lipgloss.

Ouai's Wave Spray — Used following the BB Primer, this wave spray just gives my hair the perfect texture and it's not sticky, smells amazing, unlike most wave sprays.

With that said, I would just sand down the rust areas, use some sealer / primer, then follow with rattle cans of your color of paint.

Valid Hungarian MOT certificate till 15 July 2019Accident free, in garage stored, regularly maintained, fully renovated in the last 4 years, used as hobby vehicle since the renovation.The following parts have been replaced / restored: • crankshaft and connecting rods • clutch • shift cable • oil pump • control (cam belt, valve control, tightener etc.) • cooler fans (all 3) • oil coolers (upgraded to a bigger than the original) • turbo restoration and vacuum pipes • exhaust system replaced, then upgraded to a dual (one on both sides) exhaust system • brakes (disc and shoe) • RC injectors (4x primer 370cc and 2x seconder 270cc) • electronic window motors • electric power assisted steering • A / C • new leather interior (including dashboard, seats, door and roof panels etc.) • LED backlit dashboard • carbon dashboard • carbon front light frames • secondary door seal and door glasses • front spoiler rubber lip • blanking capsI would recommend my Lotus for anyone who would like to preserve its unique condition, especially for collectors or as a hobby vehicle.The car can be inspected / picked up in Budapest, Hungary.

The following story is used with permission of Carson Dunlop, providing inspection services and home inspector training, education and reporting systems... Read more of this Home Inspector Continuing Education story Mold: Finding the Water This is the second of a two part mold «primer» by leading expert Laurence Lee, CIH, on what mold is, how and why it grows and where to look for it.

A famous example of deductive reasoning, which Nena Street repeated here in Use Logic to Win Arguments: A Primer for Lawyers, is the following syllogism:

Genotypes were determined using iPLEX Gold ™ primer extension followed by mass spectrometry analysis on the Sequenom MassARRAY system (Sequenom, San Diego, CA) by the Australian Genome Research Facility (AGRF: http://www.agrf.org.au/).

I painted the top with a grey primer I had and then used a coat of country grey AS and a coat of coco AS followed by clear wax and a light coat of dark.

But maybe a little primer on accessing and using other options for following you for those readers who don't deal with all of this every day.

I want to avoid sanding my kitchen cabinets and am considering using one of these recipes as a primer and following it with a coat of cabinet paint instead of waxing since they're heavily used.