Sentences with phrase «using gene transfer»

Treatment of malignant glioma using gene transfer modalities typically consists of surgical debulking of the tumor mass followed by the administration of the viral vectors into the brain tissue surrounding the tumor cavity.

«One way of treating patients with limb - girdle muscular dystrophy might be to use a gene transfer approach to treat the muscular dystrophy, and a pharmacological approach to treat the smooth muscle dysfunction,» he said.

Not exact matches

Then they can use classical plant breeding or genetic engineering to transfer those genes into desired cultivars.

The OAR proposal uses a variation of therapeutic cloning called altered nuclear transfer (ANT) in which the nucleus of a donor cell (a skin cell, for example), containing the 30,000 genes of the genetic code, is altered in such a way that it produces an epigenetic factor, a protein called nanog.

As a rule of thumb, plant breeders in both public research institutes and private companies use genetic engineering only in the following situations: 1) the gene of interest is not found within the crop gene pool, 2) if found in the gene pool, transfer will be difficult due to incompatibility and other breeding problems or 3) the transfer of the gene will take a long time as in the case of perennial crops like coconut.

These include genes from plants and organisms unrelated to rice that could not be transferred using other breeding methods.

Once specific genes associated with beneficial traits are identified, they can be more efficiently transferred into new rice varieties using other breeding methods.

Lee's team used a retrovirus to transfer the fluorescent gene to dog fibroblast cells, but they could not control where the virus inserted the gene.

Barroso spent his first year at GSK's Biopharmaceuticals Centre of Excellence for Drug Discovery near London looking at the use of adenoviruses in gene - transfer therapy.

In one such approach, researchers surgically remove brain cells, use viruses to transfer genes to the cells, and then graft them back into the animal's brain tissue.

Even distantly related bacteria can swap genes with one another using a variety of techniques, from direct cell - to - cell transfer, called conjugation, to transformation, in which a bacterium releases snippets of DNA that other bacteria pick up and use.

Concerns have been raised about the safety of gene therapy in the past, not least about links between the viruses used to transfer the genes and disease.

Goldstein and colleagues had reported that tardigrades imported about 17 percent of their genes from foreign sources using a type of DNA swapping known as horizontal gene transfer (SN Online: 11/25/15).

Dr. Stoddart's main research focus is using autologous stem cells and gene transfer to repair musculoskeletal tissues using a cell therapy approach.

The team's next goal was to transfer the genes for making gas vesicles from the water - dwelling bacteria into a different type of bacteria — Escherichia coli, which is commonly used in microbial therapeutics, such as probiotics.

Given that artificial synthetic siRNAs can transfer through placenta, we can try to use gene therapy to treat fetal disease by maternal administration.»

PARTICLE acts in three different ways to prevent expression of the MAT2A gene: 1) by winding around the MAT2A gene to create a DNA: RNA triple helix structure locking down the MAT2A gene promoter, 2) by binding the messenger RNA product of the MAT2A gene and preventing it being used for MAT2A protein synthesis and 3) transferring MAT2A messenger RNA into intracellular vesicles that are subsequently ejected from the cell.

Transfer RNA (tRNA) suppression of nonsense mutations in prokaryotic systems has been widely used to study the structure and function of different prokaryotic genes.

Plant molecular biologists Fumiyuki Goto, Toshihiro Yoshihara, and their colleagues first isolated the ferritin gene from soybean sprouts, and then used a soil bacterium to transfer the gene into the DNA of rice cells.

They say that genes from wasps have been transferred to several butterfly species, including the monarch, by symbiotic viruses used as biological weapons by parasitic wasps.

In the new research, Prins and Liau used a technique called adoptive cell transfer, which involves extracting and growing immune cells outside of the body, then reprogramming them with a gene known as New York Esophageal Squamous Cell Carcinoma, or NY - ESO - 1.

Beyond the interest these lateral gene transfers evoke in evolutionary biology, these results highlight the risk gene transfers could cause, in case GM - parasitoid wasps are produced, as genes artificially introduced into wasp species used for biological control could be transferred into the genomes of the targeted pests.

She identified that specific killing of myeloid cells represents an additional mechanism of suppression mediated by Tr1 cells, and she contributed to the definition of a new protocol for Tr1 cell induction using IL - 10 gene transfer and in the discovery of the biomarkers of Tr1 cells.

We will adapt our strategies of gene transfer using viruses more qualifed to transduce dog cells.

Using a novel technique to genetically modify T cells for adoptive transfer, Carl June, Michael Kalos, David Porter, Bruce Levine, and colleagues at the University of Pennsylvania School of Medicine achieve clinical responses in patients with chronic lymphocytic leukemia, including two complete, durable (one year) clinical responses, accompanied by in vivo expansion and long - term functional persistence of gene - modified cells.

The goal of the Program in Human Gene Therapy is to develop gene transfer technologies and use them for hepatic gene therapy for the treatment of genetic and acquired diseaGene Therapy is to develop gene transfer technologies and use them for hepatic gene therapy for the treatment of genetic and acquired diseagene transfer technologies and use them for hepatic gene therapy for the treatment of genetic and acquired diseagene therapy for the treatment of genetic and acquired diseases.

These cells are easily accessible (therefore, they are the first ones used in the clinic), seemingly well suited for gene transfer, and used in practice with new genes to correct two types of severe combined immunodeficiency (which are briefly described in the book).

«The significance of controlled conditions in lentiviral vector titration and in the use of multiplicity of infection (MOI) for predicting gene transfer events.»

In another experiment, the researchers used viral gene transfer and increased RbAp48 expression in the DG of aged mice.

Adeno - associated viral (AAV) vectors are the most frequently used gene - transfer tools in the study of the brain and spinal cord, which together are known as the central nervous system (CNS).

Human pluripotent stem cells (hPSCs), including human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs), are known to be vulnerable to apoptosis upon various technical manipulation, such as single cell dissociation, freezing and thawing, etc., which hinder their use for clonal isolation in gene transfer, differentiation and FACS cell sorting.

In the 1980s, Dr. Kaufman's experience with gene transfer and engineering led him to become a founding scientist at Genetics Institute Inc., where he engineered mammalian cells for high - level expression of therapeutic proteins, such as clotting factors that are now used to treat individuals with hemophilia.

Specific activities include optimizing the conduct and oversight of gene transfer research, updating and interpreting biosafety policies under the NIH Guidelines Involving Research with Recombinant and Synthetic Nucleic Acids, providing critical input on governmental policies regarding dual use research, the NIH Stem Cell Registry.

Efficient gene transfer into rhesus repopulating hematopoietic stem cells using a simian immunodeficiency virus - based lentiviral vector system.

High level erythroid lineage - directed gene expression using globin gene regulatory elements following lentiviral vector mediated gene transfer into primitive human and murine hematopoietic cells.

Draft genome annotation was transferred from P. vivax P01 using RATT21 (version 1), and supplemented with the output of the Augustus22 gene finder, trained on P. vivax P01 as described in23.

Studies on horizontal gene transfer and enzymemediated DNA rearrangements lead him to postulate a theory of molecular evolution according to which nature cares actively for the promotion of biological evolution by using products of specific evolution genes as well as several intrinsic non-genetic elements.

«Now that we have the gene, we can actually use that gene sequence to temporarily silence the gene and make rye and other chromosomes pair with wheat and transfer genes by a natural method into wheat without calling it GMO,» Gill said.

AAV gene therapy vectors are emerging as the gene transfer vehicle with high potential for use in the CNS as they transduce post mitotic cells that mediate the sustained, long term gene expression required in chronic progressive diseases.

The single - gene knockout fibroblasts were successfully used for somatic cell nuclear transfer (SCNT) and resulted in live - born goats harboring biallelic mutations.

Her research initially focused on the identification and characterization of juvenile dilated cardiomyopathy in Portuguese water dogs and has led to several ongoing studies, including most recently the evaluation of various candidate genes for use in a therapeutic gene transfer approach to improve cardiac function in failing hearts.

The research team believes that the genes for bioluminescence could be isolated and then transferred to other, larger plant organisms that could be used to provide a continual source of blue light at night.

1) Plant - mediated gene silencing: Using plant - mediated gene silencing, scientists induce plants to transfer pieces of genetic material to other organisms, targeting and interfering with the interactions between plants and their pests at the genetic level.