Sentences with phrase «using haploid cell»

Here, we demonstrate the feasibility of classical genetic screening in mammalian systems by using haploid cells, chemical mutagenesis and next - generation sequencing, providing a new tool to explore mammalian genetic interactions.

Not exact matches

«Our findings should facilitate the use of animal haploid cells, making them accessible to a broader range of laboratories and technologies,» the authors conclude.

«In mammals, in the absence of haploid cells, other approaches have been used to identify key genes, such as interfering RNA, but they are sub-optimal methods.

In a breakthrough study, Blomen et al. (Science, 2015) used extensive mutagenesis to describe the complete set of essential genes in the human haploid cell line Hap1.

Here we used genome - saturated mutagenesis to create a biobank of over 100,000 individual haploid mouse embryonic stem (mES) cell lines targeting 16,970 genes with genetically barcoded, conditional and reversible mutations.

Using conditional genetic ablation of Ar in Sertoli cells, we have shown that AR signaling is required for three stages of spermatogenesis: progression through meiosis I, the differentiation of haploid round spermatids into elongating spermatids, and spermiogenesis, the release of fully differentiated elongated spermatids into the lumen of the seminiferous epithelium.