Sentences with phrase «using immunocytochemistry»

The initial aim of the pathology investigation was to carefully examine the spinal cord using immunocytochemistry and electron microscopy, as well as classical techniques, to confirm the pathology previously reported and to look for new clues to the pathogenesis and aetiology using the more modern techniques.

Intracellular expression of human immunoglobulin in HB1.F3.H2IgG NSCs was visualized using immunocytochemistry.

Additional confirmation of knockout and pluripotency was performed using immunocytochemistry (Figure 2, Panel C).

We also cultured animals in BrdU and used immunocytochemistry to detect mitotically active cells in mature retinas generated from EFTF - expressing cells.

We derived a fibroblast line from a skin biopsy from a healthy adult male (HUF1)(Figure 1A) and used immunocytochemistry to characterize the expression of cell surface markers commonly expressed on pluripotent stem cells (Figure 1B, C and D).

Not exact matches

Schmidt et al. used high - resolution proteomics, electrophysiology, biochemistry, and immunocytochemistry on wild - type and knockout cells and animals to study PMCA - interacting proteins.

Pluripotent cells were cultured in mouse Noggin protein for 5 d and immunocytochemistry was used to detect retinal specific markers.

Mitochondrial localization of complex II visualized by immunocytochemistry using ab14715.

Right eyes were investigated by electron microscopy, left eyes were used for immunocytochemistry and fluorescence light microscopy.

Immunocytochemistry was performed using standard methods as described previously [27,43].

During the 1980s, Marder led studies using antibodies and the technique of immunocytochemistry to reveal the presence of many different neuromodulators in nerve fibres around the STG of crabs, including serotonin, proctolin and GABA.

For immunocytochemistry and neurite growth assays, a plating density of 5 × 104 cells / well of a 24 - well plate was used.

The antibodies listed above in the immunocytochemistry section were also used on eye sections together with a cocktail of antibodies generated against human - specific markers (HSM) to identify human cells (the Oka blood group antigen, mouse TRA -1-85, 1 ∶ 10 (a kind gift from Peter Andrews, University of Sheffield, Sheffield, UK) together with mouse human nuclear antigen, 1 ∶ 1000, Millipore).