Sentences with phrase «using immunofluorescence»

All cases were analysed for IgA anti-gliadin (AGA) by an ELISA test and those with positive results were tested for IgA anti-endomysium antibody (EMA) using immunofluorescence.

Apoptosis and necrosis were quantified using immunofluorescence assays; bioconjugate - treated cells were early apoptotic after 4 hours, proving effective initiation of programmed cell death.

Samples reactive by ELISA testing were then re-tested using an immunofluorescence assay (IFA)[14].

Additionally, researchers examined prostate cells — normal and cancerous — using immunofluorescence, allowing them to visualize the distribution of the target molecule and look specifically at centrosomal abnormalities and growth patterns.

Using an immunofluorescence technique, these peptide - coated BNPs were shown to preferentially target inflamed joint tissue.

We used immunofluorescence and in situ hybridization to detect specific retinal cell classes.

Not exact matches

Learn about the latest approaches to assessing cancer - immune interactions («immune profiling») using FFPE tissue sections, multiplexed immunofluorescence, and cell phenotyping software

In situ hybridization and immunofluorescence was used to explore the origin and distribution of the neurons that express this family of genes.

The researchers, who released their findings in Cancer Prevention Research Journal, published by the American Association of Cancer Research, used a visualization technique — immunofluorescence — to identify stem cells in the colon of pigs that were fed either a high calorie diet or a standard diet.

«When you're doing immunofluorescence, for instance, if you're using the wrong fixation process or fixative you could really get the wrong results even with a great antibody,» he says.

Immunofluorescence microscopy was performed using a Zeiss Axioplan fluorescence microscope (Carl Zeiss, Inc., Thornwood, NY) equipped with a 100 - W Hg lamp and narrow band pass excitation filters (Chroma Technology Corp., Brattleboro, VT).

Latex bead labelling and comparative immunofluorescence analysis of the p62 subunit of the TFIIH was performed as described previously [16,17] using primary mouse embryonic fibroblasts at passages 2 — 5.

In total, more than 500 proteins were analyzed with two well - established methods used in most laboratories for biological studies; immunofluorescence (IF) and fluorescence protein (FP) tagging.

ImmunoStar's 5 - HT6R Antibody demonstrates significant labeling of rat cortex, amygdala and hippocampus and other areas using immunohistochemistry and immunofluorescence.

Patients and Methods: A tissue microarray (TMA) consisting of four biopsy cores from 34 matched pairs of nephrectomy and metastatic sites of clear cell RCC was used to assess PD - L1 expression by quantitative immunofluorescence.

All genes with Data reliability «Supported» in one or both of the two methods immunohistochemistry and immunofluorescence, or standard validation «Supported» for the Western blot application (assays using over-expression lysates not included) are classified as «Evidence at protein level».

(c)--(f) Immunofluorescence staining using Sox2 antibody.

(g)--(j) Immunofluorescence staining using Nestin anbibody.

Antibodies used for immunofluorescence staining included YAP1 from Cell Signaling and TAZ from BD Biosciences.

The antibodies used in this study includes COX - 2 (Cayman Chemicals, Ann Arbor, MI, USA), STAT6 for Western blot (Cell Signaling Technology, Danvers, MA, USA), for immunofluorescence (IF)(BD Pharmingen, San Diego, CA, USA), for ChIP (Santa Cruz Biotechnology, Santa Cruz, CA, USA), phospho - STAT6 (Cell Signaling Technology), p300 for Western blot, (Upstate, Lake Placid, NY, USA), for ChIP (Santa Cruz Biotechnology), normal rabbit IgG (Santa Cruz Biotechnology), mouse immunoglobulin (Ig) G1, κ Ig (BD Pharmingen), glyceraldehyde -3-phosphate dehydrogenase (Advanced ImmunoChemical, Long Beach, CA, USA), Cy3 - conjugated AffiniPure Goat anti-mouse IgG (Jackson ImmunoResearch, West Grove, PA, USA) and PARP (Cell Signaling Technology).

Frozen sections of lung and colon tissues from wild - type mice and Slc6a14 − / − mice were also used for immunofluorescence analysis of Slc6a14 protein expression with an antibody raised against a mouse peptide sequence [17].

Additional blinded screening of the 51 cases and 53 controls at the Robert Koch Institute using an ELISA employing recombinant Gag and Env XMRV proteins identified weak seroreactivity in one CFS case and a healthy control, which was not confirmed by immunofluorescence.

Overlaying phase contrast and SSEA3 immunofluorescence images revealed that the SSEA3 expression was detected across the entire cell surface (Figure 1E) and using confocal microscopy we observed that the SSEA3 expression was primarily localized to the cellular membrane (Figure 1F).

Bulk - cultured PC3 or PC3 - GFP cells, or clone - derived cultures were plated on glass coverslips and used in immunofluorescence staining for CD44, α2β1 integrin, and β - catenin using monoclonal antibodies and protocols described previously (27 — 29).

High - throughput profiling of protein targets in single cells using multiplexed immunofluorescence and fluorescent bioimaging.

Cells used for immunofluorescence analysis were fixed immediately following overnight adherence to remove dead and non-viable cells and cells used for transcriptional analysis were cultured for 6 days prior to analysis.

After binding, cells were washed once (carefully using a 200 μl pipette to remove the medium) with RPMI with 0.5 % BSA solution and adherent cells were analyzed by immunofluorescence microscopy or bright field microscopy for quantification.

FIV antibodies can be detected using a number of techniques, including enzyme - linked immunosorbent assay (ELISA), western blot, and immunofluorescence (IFA) assays.

Immunofluorescence is sometimes used, for example.

Hemagglutination, ELISA, and immunofluorescence antibody tests are available, and PCR is highly sensitive and specific when used on fresh tissue and fluid samples.

Key Highlight: • Handled flourochrome labeled antibody staining used for flowcytometry, cell proliferation assay, immunofluorescence cell staining and cytokine assays using ELISA.