Sentences with phrase «using immunoprecipitation»

Quantitative serum AChR antibody concentrations were determined as previously described using an immunoprecipitation radioimmunoassay procedure [22].

By using immunoprecipitation and mass spectrometry, we identified autoantibodies anti-Adenylate kinase 5 (AK5 - Abs) as specific marker in 10 patients with LE.

For the coimmunoprecipitation experiments, HuH - 7 cells transfected with pcDNA - SRα / FLAG - Sp 1 were harvested and cell lysates were prepared using immunoprecipitation lysis buffer [20 mmol / L Tris - Cl (pH 7.5), 150 mmol / L NaCl, 10 % glycerol, and 1 % Triton X-100].

Not exact matches

To measure bits of amyloid coursing through the bloodstream, Yanagisawa and colleagues used a technique called immunoprecipitation with mass spectrometry, which deploysantibodies to bind and identify proteins.

One current method commonly used today is called chromatin immunoprecipitation, combined with next - generation gene sequencing (ChIP - seq).

At ABclonal, we have nearly 100 existing chromatin immunoprecipitation (ChIP)- validated antibodies with a higher specificity than most commercial antibodies, which are used for a wide range of epigenetics studies, from those investigating developmental processes to those researching various diseases.

To this end, using knockout cells in combination with siRNA and immunoprecipitation approaches, our results revealed that Pen - 2 together with presenilin are sufficient... to form a functionally active enzyme to process Notch.

After extensive washing with immunoprecipitation lysis buffer, the immunoprecipitated proteins were analyzed by immunoblotting using specific antibodies against DDX3 and FLAG epitope (ab1257, Abcam, Cambridge, United Kingdom).

Hanner and Rusche used chromatin immunoprecipitation assays to show that this chimeric protein did not make it to the HMRa or HMRα loci or to other repressed genes.

Samples can be frozen at -80 °C for long - term storage, or be used for immediate Western blotting or immunoprecipitation.

All chromatin immunoprecipitations were quantified using quantitative PCR.

To evaluate whether loss of ABL kinases affected TAZ activity, we performed chromatin immunoprecipitation (ChIP) analysis using primers for TAZ targets identified by ChIP sequencing analysis (42).

For Western blot assay and immunoprecipitation analysis, CD4 + T cells were isolated from mice spleen on day 21 after transplantation using a Mouse CD4 + T Cell Isolation Kit (Miltenyi Biotec), and the procedures were performed as described previously (35).

Chromatin immunoprecipitation was used to monitor Nrf2 and Bach1 occupancy at the HMOX1 E2 ARE.

We confirmed that these compounds modulate Bach1 directly using chromatin immunoprecipitation and reporter assays.

We will map the mRNA interactome of DDX6 and mutant variants using protein - RNA crosslinking, immunoprecipitation, and next - generation sequencing to identify mRNAs where DDX6 binds, the region within the mRNA, and how the repertoire of putative targets changes.

A diverse portfolio of high - quality primary and secondary antibodies is available for use in cancer research, epigenetics, immunology, neuroscience, and stem cell research as well as validated for multiple applications, including flow cytometry, western blotting, and immunoprecipitation.

His group uses high - throughput genomic methods — including DNA sequencing, genotyping, chromatin immunoprecipitation, mRNA expression profiling, transcriptional promoter and DNA methylation measurements — as well as computational and statistical tools to identify, characterize and understand the functional elements encoded in our genomes and how they work together at the molecular level in normal and pathological conditions.

Normal rabbit IgG (Millipore) was used to control the nonspecific immunoprecipitation of chromatin by immunoglobulins.

To investigate whether unphosphorylated STAT6 binds to this site, chromatin immunoprecipitation (ChIP) assays using anti-STAT6 antibody were performed and demonstrated that unphosphorylated STAT6 bound to this consensus site (Figure 5a).

Chromatin immunoprecipitation (ChIP) assay was performed using EZ ChIP kit (Millipore) according to the instruction of the manufacturer.

June 4, 2015 New tool brings standards to epigenetic studies One of the most widely used tools in epigenetics research — the study of how DNA packaging affects gene expression — is chromatin immunoprecipitation (ChIP), a technique that allows researchers to examine interactions between specific proteins and genomic regions.

Abs used for Western blotting and immunoprecipitation were anti-phospho Syk, - phospho PLCγ1, - phospho PLCγ2, - ERK, - phospho p38, - p38, - Lyn (Cell Signaling Technology), anti-phospho SLP76 and - phospho LAT (BD Biosciences), anti-phospho tyrosine (clone pY20, Neomarkers), anti-phospho tyrosine (clone 4G10), - myc and - HA (Roche) and - FcRγ (Millipore).

When an antibody to endogenous SUV39H1 was used for immunoprecipitation, MeCP2 was effectively coimmunoprecipitated; conversely, aHA antibodies to HA - tagged MeCP2 could immunoprecipitate SUV39H1 (Fig. 2G).»

Synthesized and purified proteins using chromatography methods, gel electrophoresis, immunoprecipitation and other scientific methods.

A detailed description of the methods and analyses of methylated DNA immunoprecipitation (MeDIP) and microarrays hybridization used in this study were previously described [43] and can be found in File S1.