Sentences with phrase «using isotype»
Secondary labeling was carried out using isotype matched Alexa - fluor labeled secondary antibodies (AF488, 568 and 594, all from Invitrogen Corp).
http://journals.plos.org/ Not exact matches
Use negative controls (unstained and isotype control antibodies) to determine gating of populations.
Primary isolates that were incubated with identical concentrations of FITC - and PE - conjugated isotype standards were used to assess the level of background intensity.
An isotype control for the secondary antibody was used as a negative control in both samples.
Therefore, it is critical to use a secondary antibody that specifically recognizes that isotype.
Therefore, to maximize detection of the target, it is best to use a secondary antibody that recognizes all isotypes.
Isotype control antibody (black line) was rabbit IgG (monoclonal)(1μg / 1x106 cells) used under the same conditions.
Isotype control antibody (black line) was mouse IgG1 [ICIGG1](ab91353, 2µg / 1x106 cells) used under the same conditions.
An Alexa Fluor ® 488 conjugate with isotype specificity to the mouse antibody was used as a secondary antibody.
But once the isotype batteries are used up they will be disposed of only through authorized agents of the manufacturer.