Sentences with phrase «using macrophages»

Here, we describe an approach using macrophages derived from human induced pluripotent stem cells (iPSdMs) to study macrophage - Chlamydia interactions in vitro.

Drs. Merad and Mortha are now developing a multiscale approach to identify GM - CSF downstream targets using macrophages and dendritic cells isolated from GM - CSF deficient mice and exposed to recombinant GMCSF.

Personally, if I wanted to use a macrophage activating compound, then I would choose Bravo yogurt.

Unfortunately for the new host, this process does not damage the virus as intended enabling the virus to use the macrophage as a means of transportation through the host's body.

Not exact matches

This is due to a randomised trial with 215 eligible patients performed between 1989 and 1991, which showed a significant increase in severe thrombocytopenia, severe anemia, pulmonary complications and toxic deaths when granulocyte - macrophage CSFs (GM - CSFs) were used during concurrent chemo - radiotherapy.

«We came up with a list of potential cellular candidates that could be compensating for the loss of these specific macrophages and used several different tests to determine which cells were clearing away the nerve debris after injury,» Lindborg said.

For the research, the scientists used the platform to target colon macrophages in order to reduce inflammatory symptoms caused by Inflammatory Bowel Disease (IBD) in mouse models.

«This is really the first time that it's been demonstrated that you can use a rapid methodology like this to spatially define cancer cells and macrophages,» Kilian said.

Kilian said these questions range from the basic — how macrophages signal to the breast cells — to the more long - term: Can therapeutics be used to disrupt that communication?

Kemp and Payne have already used the tool to visualize the signaling process that takes place as macrophages discover bacteria and move to engulf and destroy the invaders.

The researchers used a new cell tracing method to follow the movement of peritubular macrophages from the bone marrow to the testes.

Using genome - wide approaches, they found that the Rev - Erb proteins repressed gene expression in macrophages primarily by binding to enhancers.

To further untangle what was going on, Chawla, his postdoc Yifu Qiu, and their colleagues used mice that lacked interleukin - 4 (IL - 4) and interleukin - 13, proteins that help activate macrophages.

This visual abstract represents the findings of Thevaranjan et al. who, using young and old germ - free and conventional mice, demonstrate that age - related microbiota changes drive intestinal permeability, age - associated inflammation, and decreased macrophage function.

Using mice deficient in Del - 1, they found that the protein promotes proliferation and differentiation of hematopoetic stem cells, sending more of these progenitor cells down a path toward becoming myeloid cells, such as macrophages and neutrophils, rather than lymphocytes, such as T cells and B cells.

HIV - 1 infects and kills immune system cells, including T cells and macrophages that were used in the study.

«It's really about finding different ways to develop better vaccines, ones that will harness the power of macrophages and finally put the body's innate immune memory to use» says Dr. Barreiro.

Using mice that had been manipulated to develop MS, the researchers discovered that chronic MS leads to significantly increased levels of succinate, a small metabolite that sends signals to macrophages and microglia, tricking them into causing inflammation, but only in cerebrospinal fluid, not in the peripheral blood.

Researchers at Emory University School of Medicine were testing whether GM - CSF (granulocyte - macrophage colony stimulating factor), already used to restore white blood cell numbers during cancer treatment, could help heal blood vessels damaged by atherosclerosis.

Macrophages stained with F4 / 80 - APC were separated from F4 / 80 — cells using a FACSAria cell sorter (BD Biosciences Immunocytometry Systems Inc., San Jose, California, USA).

Quantitative RT - PCR was used to measure the relative expression of macrophage markers (Emr1, Csf1r, Cd68) and an adipocyte - specific gene (Acrp30).

Using quantitative RT - PCR we determined the relative expression levels of the macrophage expressed gene Cd68 in human abdominal subcutaneous adipose tissue.

Macrophages are immune cells commonly used as markers for inflammation, and their increased presence helps confirm that SNRK plays a role in regulating inflammation in white fat tissue.

The project began when a post-doc in the Mullins lab, Lillian Fritz - Laylin, collected a time - series of images of macrophage - like immune cells in motion using Betzig's new microscope.

Among the strengths of the study: instead of using just one method to deplete macrophages, the researchers used nine distinct methods to deplete different myeloid cells in various locations, allowing them to pinpoint the most important cell type and its primary location of action.

Hematopoietic malignancies were diagnosed using the following markers: for B - and T - lymphocyte detection, rat antimouse CD45R / B220 mAb (Southern Biotechnology Associates, Birmingham, AL; dilution 1:100); for T - lymphocyte detection, rabbit antihuman CD3 polyclonal antibody (Dako, Glostrup, Denmark; 1:100); and for monocyte / macrophage detection, rat antimouse F4 / 80 mAb (BMA Biomedicals AG, Augst, Switzerland; 1:20) and rat antimouse CD11b mAb (Chemicon International, Temecula, CA; 1:20).

Mechanical properties of many different cell types including glial cells, platelets, cardiocytes, macrophages, endothelial cells, epithelial cells, fibroblasts, bladder cells and more have been determined using F - d spectroscopy.

We, therefore, treated macrophages of six AD patients and 3 controls by curcuminoids in vitro and measured Aβ uptake using fluorescence and confocal microscopy.

Results: Scientists at Pacific Northwest National Laboratory (PNNL) have reported the first systematic investigation using global proteomics to identify changes in the abundance of bacterial proteins associated with macrophage colonization and a new protein that helps the bacterium to survive inside macrophages.

The Dotiwala laboratory uses cellular, molecular, biochemical and omics approaches, as well as sophisticated in vivo imaging, to dissect the mechanism by which cell - based immunity (neutrophils, macrophages and killer lymphocytes) is activated in the presence of different pathogenic bacteria, such as intra - and extracellular bacteria, fungi and protozoa.

Protective antigen (PA)- Alexa647 at indicated concentrations: 0 ug / ml (red), 0.01 μg / ml (blue), 0.1 μg / ml (green), 1 μg / ml (orange) and 10 μg / ml (cyan) were used to determine the binding to ILC3 or RAW264.7 mouse macrophages.

However in the case of HIV, macrophages are used by the virus, which efficiently infects them and makes them produce new virions.

By infecting susceptible mouse macrophages with S. Typhimurium and using global proteomics to analyze the proteins at various time points following infection, researchers identified 1,006 macrophage proteins.

Using primary murine bone marrow derived macrophages or human monocyte derived dendritic cells, infected with live or attenuated Chlamydia trachomatis we report that the live organism activates both canonical and non-canonical inflammasomes, but only canonical inflammasomes controlled IL - 1β processing which preceded pyroptosis.

When they used such mice to test their approach, they found that the bulk of therapeutic effect from CD47 blockade relied not on macrophages but on dendritic cells.

By changing the mouse model they use to study how the immune system responds to cancer, a team of researchers hopes to shift the focus for one form of cancer immunotherapy back to the standard approach — relying on antigen - presenting dendritic cells — and away from the current upstart, macrophages.

Using CRISPR / Cas9 technology, we generated biallelic knockout mutations in host genes encoding IRF5 and IL - 10RA in iPSCs, and confirmed their roles in limiting chlamydial infection in macrophages.

The Stanford investigators found that when they used antibodies against CD47 to negate this «don't - eat - me» signal, macrophages were able to chew up many of these cancer cells.

A loss of Vγ4 + Ccr6 + cells was also observed following CD169 + macrophage ablation using CD169 - DTR mice (Figure 7F).

The positions of both Cxcr6 + cells and CD11b + subcapsular macrophages were determined using spots objects in Imaris.

It has long been known that antibiotics suppress various immune functions, and especially macrophage activities such as chemiluminescence response, chemotactic motility, bactericidal and cytostatic ability and similar negative effects have also been seen with other commonly used drugs such as H2 - blockers, proton inhibitors and surface - protection agents.

If you're making or using excess insulin, the switch to a fat burning metabolism allows the insulin to normalise and causes your cells, including the macrophages, to let go of cholesterol — and when they do, the lipoproteins are there ready to carry it away.

It is also characterized by a cellular reaction focused on the use of neutrophils and macrophages to «clean up» the site through debridement, phagocytosis, matrix synthesis, and cell recruitment and activation.

Regardless of the techniques used, the alveolar macrophage is an absolutely normal finding and should not be interpreted as a sign of bronchopulmonary inflammation or pathology.

The drugs used for this are toxic to the animal, people and the environment so it has sometimes been combined with immunotherapy (using cells to stimulate the macrophages of the immune system).

For years, researchers have been trying to develop a way for the spinal cord to repair itself — and they may have broken new ground with the use of macrophages.