Spleen cells were enriched for CD8 + and CD8 − T cells
using magnetic beads.
The kit adopts a novel purification method,
using magnetic beads and phosphatidylserine - binding protein to isolate exosomes and other EVs from cell - culture medium and body fluids by a normal microcentrifuge.
So the researchers turned to a machine made by Quanterix of Lexington, Massachusetts,
that uses magnetic beads coated by an antibody that binds selectively and extremely tightly to the protein in the blood.
«
We used magnetic beads of 3 micrometers in diameter to pull down the exosomes in plasma samples,» Zeng said.
He still likes to tinker; only now
he uses magnetic beads, atomic - force microscopes, and laser «tweezers» to explore the inner workings of the cell and the physical forces behind DNA replication.
Not exact matches
Magnetic beads have long been
used to isolate nucleic acids as well as recombinant proteins.
The plasma containing
magnetic beads then flows through the microchannels on the diagnostic chip in which the
beads can be readily collected
using a magnet to extract circulating exosomes from the plasma.»
A team led by the Hutch's Marie Bleakley and by Warren Shlomchik of the University of Pittsburgh School of Medicine in Pennsylvania removes naïve T cells from grafts with a
magnetic system that
uses monoclonal antibodies bound to iron
beads.
Given these different methylation patterns, the researchers
used specialized
magnetic beads that hybridize with methyl - CpG - rich DNA regions in an attempt to separate nuclear DNA from organellar DNA in total genomic DNA samples.
The putative DN cells were isolated by negative selection
using anti-CD8
magnetic bead isolation followed by in vitro treatment with anti-CD8 antibody and complement.
Using a proprietary protocol DNA fragments are bound directly onto the surface of the
magnetic beads, leaving unincorporated nucleotides, primers, primer dimers, and other contaminants in solution.
In a single reaction volume, Luminex has the ability to perform many different tests and
use either
magnetic beads or plain polystyrene
beads.
Target bacteria were captured
using antibody - coated
magnetic beads and then encapsulated in picoliter - sized droplets with fluorescently labeled antibodies for detection.
For sorting human ILCs, tonsillar lymphocytes were depleted of B cells
using CD19
magnetic beads (eBioscience).
With MagSi - Direct, you can start with a molecule of interest, attach
magnetic beads to it and then
use a magnet to isolate that molecule from any desired reaction mixture, together with any other molecules, complexes or even intact cells to which the starting molecule has bound.
Cellular biology: Isolation and culture of various cell types; cryopreservation; Mixed Lymphocyte Reaction (MLR); cloning by limiting dilution; cytotoxicity assays
using Chromium - 51 release; proliferation assay (CFSE and 3H - thymidine); ELISA; Elispot;
magnetic beads separation.
Using the new solution, DNA fragments are bound directly onto the surface of the
magnetic beads, leaving unincorporated nucleotides, primers, primer dimers, and other contaminants in solution.
HIF -1-alpha was immunoprecipitated
using 0.5 mg HeLa Nuclear DFO treated whole cell extract (ab180880), 5µg of Rabbit polyclonal to HIF1 alpha and 50µl of protein G
magnetic beads (+).
Following lysis and solid - phase capturing of mRNAs with streptavidin
magnetic beads, samples were reverse transcribed
using random primers.
HIF - 1 alpha was immunoprecipitated
using 0.5 mg HeLa Nuclear DFO treated whole cell extract (ab180880), 5 µg of Mouse monoclonal to HIF - 1 alpha and 50 µl of protein G
magnetic beads (+).
Using the dyes with
magnetic nanoparticles and tiny fluorescent
beads, allowed them to
use the slime mold network as a biological «lab - on - a-chip» device.
STRATEGIES COVERED Subitizing Counting on and counting back
Use five / use ten Use doubles Get to ten Use ten and adjust Get to ten Use known facts Splitting Use a friendly number Get to a friendly number Give and take Over and adjust Find the distance Keep the same distance INCLUDES 1 Teacher Guide 3 Anchor Charts 120 Number Strings 60 Math Talks 1 Set of 5, 10, Double 10 Frame Cards 20 - Bead Demonstration Rekenrek 120 Bead Demonstration Magnetic Rekenrek L
Use five /
use ten Use doubles Get to ten Use ten and adjust Get to ten Use known facts Splitting Use a friendly number Get to a friendly number Give and take Over and adjust Find the distance Keep the same distance INCLUDES 1 Teacher Guide 3 Anchor Charts 120 Number Strings 60 Math Talks 1 Set of 5, 10, Double 10 Frame Cards 20 - Bead Demonstration Rekenrek 120 Bead Demonstration Magnetic Rekenrek L
use ten
Use doubles Get to ten Use ten and adjust Get to ten Use known facts Splitting Use a friendly number Get to a friendly number Give and take Over and adjust Find the distance Keep the same distance INCLUDES 1 Teacher Guide 3 Anchor Charts 120 Number Strings 60 Math Talks 1 Set of 5, 10, Double 10 Frame Cards 20 - Bead Demonstration Rekenrek 120 Bead Demonstration Magnetic Rekenrek L
Use doubles Get to ten
Use ten and adjust Get to ten Use known facts Splitting Use a friendly number Get to a friendly number Give and take Over and adjust Find the distance Keep the same distance INCLUDES 1 Teacher Guide 3 Anchor Charts 120 Number Strings 60 Math Talks 1 Set of 5, 10, Double 10 Frame Cards 20 - Bead Demonstration Rekenrek 120 Bead Demonstration Magnetic Rekenrek L
Use ten and adjust Get to ten
Use known facts Splitting Use a friendly number Get to a friendly number Give and take Over and adjust Find the distance Keep the same distance INCLUDES 1 Teacher Guide 3 Anchor Charts 120 Number Strings 60 Math Talks 1 Set of 5, 10, Double 10 Frame Cards 20 - Bead Demonstration Rekenrek 120 Bead Demonstration Magnetic Rekenrek L
Use known facts Splitting
Use a friendly number Get to a friendly number Give and take Over and adjust Find the distance Keep the same distance INCLUDES 1 Teacher Guide 3 Anchor Charts 120 Number Strings 60 Math Talks 1 Set of 5, 10, Double 10 Frame Cards 20 - Bead Demonstration Rekenrek 120 Bead Demonstration Magnetic Rekenrek L
Use a friendly number Get to a friendly number Give and take Over and adjust Find the distance Keep the same distance INCLUDES 1 Teacher Guide 3 Anchor Charts 120 Number Strings 60 Math Talks 1 Set of 5, 10, Double 10 Frame Cards 20 -
Bead Demonstration Rekenrek 120
Bead Demonstration
Magnetic Rekenrek Line
STRATEGIES COVERED Splitting
Use a friendly number Get to a friendly number Give and take Over and adjust Find the distance Keep the same distance Double
Use partial products
Use five times
Use ten times Double and half Factor and group flexibility Multiply up Partial quotients
Use relationships INCLUDES 1 Teacher Guide 3 Anchor Charts 120 Number Strings 60 Math Talks 1 Set of Picture & Dot Multiplication Cards 7 Flexible Grid Arrays 120
Bead Demonstration
Magnetic Rekenrek Line