Sentences with phrase «using microinjections»

Transgenic lines were prepared using microinjections into gonads of young adult N2 hermaphrodites as described (Tabara et al., 1999; Timmons, Court & Fire, 2001; Vohanka et al., 2010).

Using microinjection of adeno - associated viral vector bearing a transgene encoding FKBP1b into the hippocampus of aged male rats, we assessed the critical prediction that overexpressing FKBP1b should reverse Ca2 + - mediated manifestations of brain aging.

Not exact matches

«It proves that microinjection can be used in large animals and livestock.»

A flat tip microinjection pipette with an internal diameter of 15 — 18 µm was used for iPS cell injection.

We identified an efficient OCT4 - targeting guide RNA using an inducible human embryonic stem cell - based system and microinjection of mouse zygotes.

This product was similarly purified in a low - melt agarose gel and used at 1 ng / μl along with 50 ng / μl of the unc - 36 (+) cosmid derivative RIp16 (gift of L Lobel) for microinjection into unc - 36 -LRB--) animals.

This technology requires technical skills that are typically not offered by veterinary practices and includes aspiration of immature or mature eggs from mares using ultrasound - guided transvaginal aspiration (TVA) of follicles, in vitro culture of the eggs, micromanipulation and microinjection of eggs with a single selected sperm, and embryo culture in the laboratory, with freezing, and transfer of embryos to synchronized recipient mares.