Sentences with phrase «using nucleic acids»

Scientists are currently investigating ways of using nucleic acids to re-program cells to carry out different functions.

Using nucleic acids can mitigate or eliminate this problem, especially with the high degrees of multiplexing seen in the platform of Olink Proteomics, headquartered in Uppsala, Sweden.

For this reason, the US Food and Drug Administration has recommended screening of donor blood using a nucleic acid test (NAT).

Not exact matches

This blockchain manager would «compute a cryptographic hash» of a given «nucleic acid sequence» and «use» it as PoW before transmitting the attendant «new block... to the other nodes in [the] blockchain system.»

The tests use the so - called polymerase chain reaction (PCR), a conventional method for detecting nucleic acid.

To start, she used a cotton swab to get a sample of cells from her cheek, boiled them in a test tube in her kitchen to free the DNA, then added primers, nucleic acids that mark the part of the sequence.

The miR - 182 was safely delivered to the tumors using spherical nucleic acids, DNA and RNA arranged around a gold nanoparticle center.

Using a combination of old - fashioned clinical observation and modern biochemical analysis, he has shown that a person's appetite and their eating behavior can be linked to specific genes — and that even a tiny defect such as the absence of a single nucleic acid in a sequence of DNA can lead to runaway weight gain.

Magnetic beads have long been used to isolate nucleic acids as well as recombinant proteins.

Tests using floating nucleic acids could diagnose disease, monitor pregnancy and weed out «mad» cows

In a trial described in Nature Materials, the team showed they could deliver the nucleic acids DNA and siRNA into human cells in the lab, using the nanoneedles.

The scientists at the Institute for Research in Biomedicine (IRB Barcelona) describe the uses of the program in an article published in the journal Nucleic Acids Research, in which they explain that ChroGPS is the answer to a problem that has been dragging on for the last ten years.

Most of the prize money will go to new research and a company party, but CureVac also plans to use some of it to build an exhibit honoring Friedrich Miescher, a 19th century Swiss scientist whose discovery of nucleic acids isn't widely known.

Now, Wyss Institute researchers led by Church have developed a new suite of such sensors, reported in Nucleic Acids Research journal, that not only increase the number of cellular «switches and levers» that scientists can use for complex genetic re-programming, but also respond to valuable products such as renewable plastics or costly pharmaceuticals and give microbes a voice to report on their own efficiency in making these products.

In the study published in Nucleic Acids Research, the researchers were able to induce and inhibit the expression of genes in mammalian cell cultures and were able to regulate intracellular protein levels using light signals.

Led by Dr. Peter Glazer, chair of therapeutic radiology, Dr. Mark Saltzman, chair of biomedical engineering, and Dr. Marie Egan, professor of pediatrics and of cellular and molecular physiology, the collaborative team used synthetic molecules similar to DNA — called peptide nucleic acids, or PNAs — as well as donor DNA, to edit the genetic defect.

Nogales and her team use structural biology to elucidate how biomolecules, particularly proteins and nucleic acids (DNA, RNA), are organized and combine to form functional biological assemblies.

Proteins, fatty acids, and nucleic acids are essential factors for cell survival using glutamine metabolism.

Clustered regularly interspaced short palindromic repeats (CRISPR) / CRISPR - associated (Cas) systems provide bacteria and archaea with adaptive immunity against viruses and plasmids by using CRISPR RNAs (crRNAs) to guide the silencing of invading nucleic acids.

Using sensitive polymerase chain reaction (PCR) tests to scour through Brown's blood cells, plasma, and rectal tissue, Yukl and others were able to were able to detect bits of viral nucleic acid.

However the team from the Krebs Institute for Nucleic Acids at the University of Sheffield found that the key to preventing resistance to a common class of chemotherapy used to treat breast and colon cancer is to change the speed in which the cancer cells repair damage to their DNA that is introduced by chemotherapy.

«We identified a set of novel microRNAs that are potent inducers of neuroblastoma cell differentiation and found that mimics (synthetic fragments of nucleic acid used to raise microRNA levels in cells) of some of the identified microRNAs are much more potent in inducing neuroblastoma cell differentiation than the current differentiation treatments.

In their report receiving advance online release in Nature, the investigators describe evolved versions of the DNA - cutting Cas9 enzyme that are able to recognize a different range of nucleic acid sequences than is possible with the naturally occurring form of Cas9 that has been used to date.

The isolated intact exosomes and other EVs can be used in electron microscope analysis; nanoparticle tracking analysis; administration of EVs; and analysis of proteins, lipids, or nucleic acids.

Extracted nucleic acid was amplified using a random PCR method to generate cDNA libraries for Virochip and deep sequencing analyses as previously described [18], [21].

The TMAdV - specific quantitative real - time PCR was performed on a Stratagene MX3005P real - time PCR system using a 25 µL master mix consisting of 18 µL of water, 2.5 µL of 10X Taq buffer, 1 µL of MgCl2 (50 mM), 0.5 µL of deoxynucleoside triophosphates (dNTPs; 12.5 mM), 0.5 µL of each primer (10 µM), 0.5 µL SYBR green, 0.5 µL of Taq polymerase (Invitrogen, Carlsbad, CA), and 1 µL of extracted nucleic acid.

In the longer run, maybe nucleic acid based drugs will become heavily used once improved delivery systems are discovered.The second big change is to agriculture.

Next they isolated the nucleic acids, DNA and RNA, using alcohol.They then destroyed the RNA using the RNase enzyme, leaving just the DNA behind.

«My favorite is the one we cited in the manuscript: «Viruses are entities whose genomes are elements of nucleic acid that replicate inside living cells using the cellular synthetic machinery and causing the synthesis of specialized elements that can transfer the viral genome to other cells.

For the positive control, nucleic acid extracted from virus stocks was used.

Specific activities include optimizing the conduct and oversight of gene transfer research, updating and interpreting biosafety policies under the NIH Guidelines Involving Research with Recombinant and Synthetic Nucleic Acids, providing critical input on governmental policies regarding dual use research, the NIH Stem Cell Registry.

Proteins, nucleic acids and their complexes are then crystallized and analysed by X-ray diffraction, using synchrotron radiation.

He'd like a way to perform single - cell analysis using microarrays, instead of full sequencing, but chip - based bisulfite methods require more nucleic acid — about a microgram — than one cell can provide.

Ronald W. Davis, Ph.D., Stanford University, Stanford, Calif. $ 450,000 (2 years) «Single Molecule Nucleic Acid Detection with Nanopipettes» This group takes a stepwise approach to single nucleic acid molecule detection, using the nanoscale pore in a pulled glass pipette to measure single DNA molecules with attached nanoparNucleic Acid Detection with Nanopipettes» This group takes a stepwise approach to single nucleic acid molecule detection, using the nanoscale pore in a pulled glass pipette to measure single DNA molecules with attached nanoparticAcid Detection with Nanopipettes» This group takes a stepwise approach to single nucleic acid molecule detection, using the nanoscale pore in a pulled glass pipette to measure single DNA molecules with attached nanoparnucleic acid molecule detection, using the nanoscale pore in a pulled glass pipette to measure single DNA molecules with attached nanoparticacid molecule detection, using the nanoscale pore in a pulled glass pipette to measure single DNA molecules with attached nanoparticles.

Her doctoral research examined novel separations of nucleic acids using ion - pairing reversed - phase high performance liquid chromatography (IP - RPLC).

For high - throughput screening using fragment analysis, RNA was isolated using a MagnaPure LC system with a MagnaPure LC total nucleic acid isolation kit (Roche) according to instructions from the manufacturer.

More recently, Mirkin lab has developed a novel version of spherical nucleic acids, named Sticky - Flares (Briley et al., PNAS 112:9591 - 9595, 2015), which has also been patented for commercial use.

The Laboratory of Protein Structure (LPS) started its activities in 2008 and focuses on structural and biochemical studies of proteins (e.g.: nucleic acid enzymes) using protein crystallography as the primary method.

Total RNA was isolated using the RNeasy Mini Kit from Qiagen and treated with RNase - free DNase I (5 units / 100 µg of nucleic acids, Sigma).

As noted above, existing cancer vaccines that use dendritic cells require extracting cells from a patient's blood, treating them with an engineered protein or nucleic acid that combines tumor antigens with immune - stimulating molecules, and returning the activated dendritic cells to the patient.

We developed a way of getting siRNAs into epithelial cells by either mixing them with a transfection lipid used to introduce exogenous nucleic acids into cells in the lab or by adding a cholesterol tag to the end of the RNA sequence that allowed the RNA to be taken up into cells.

Our group seeks to investigate the use of chemically - modified nucleic acid aptamers, single stranded DNA or RNA sequences that specifically bind to a diverse variety of targets, in biosensing and catalysis.

This special carrier can also be potentially used to co-deliver therapeutic nucleic acids to prevent cancer cells from developing resistance to multiple drugs4.

Any large deletions, or other rearrangements of part of a chromosome can be identified, and using modern nucleic acid probes, individual cloned genes can be placed on the polytene map.

Led by Dr. Peter Glazer, chair of therapeutic radiology, Mark Saltzman, chair of biomedical engineering, and Dr. Marie Egan, professor of pediatrics and of cellular and molecular physiology, the collaborative team used synthetic molecules similar to DNA — called peptide nucleic acids, or PNAs — as well as donor DNA, to edit the genetic defect.

The sample was injected with live cell nucleic acid stain [STYO 13] which is taken up by live bacteria and shows as bright green when viewed using a 500 nm - long pass emission filter for fluorescence detection.

Made using the new spec for OCR Biology A 2.1.3 Nucleic acids topic Lessons included: 1.

Polymerase chain reaction (PCR) can be used to detect nucleic acids.