Sentences with phrase «using oligo»

Orders must be submitted using the Oligo Ordering Form.

After DNase treatment, total RNA concentration was quantified and 1 μg total RNA was converted to cDNA using oligo dT and reverse transcriptase.

RNA was isolated from cancer cells using the RNeasy RNA isolation kit (Qiagen), and complementary DNA was synthesized using oligo (dT) primers and Moloney murine leukemia virus reverse transcriptase (Invitrogen).

gRNAs targeting every gene in the human genome for example, can be made easily and cheaply using oligo library synthesis.

We computed Robust Multichip Average expression measures using the Oligo package in Bioconductor (22).

Not exact matches

I use one of the fancy pipettes to move the oligo into the plasmid tube, and I also add buffer, water, and an enzyme.

cDNA synthesized by reverse transcription of 1 µg RNA primed with oligo (dT) and random 9 - mer primers was used as the substrate for quantitation of mRNA expression levels by quantitative RT — PCR in the presence of SYBR ® Green (Stratagene, La Jolla, CA).

cDNA was prepared from total RNA by reverse transcription using standard protocols for the SuperScript ® III First - Strand Synthesis System (Invitrogen) and oligo (dT) priming.

RNA was DNase treated (Roche) and cDNA was reverse transcribed using Transcriptor (Roche) with oligo dT as the primer.