Sentences with phrase «using organ culture»

Not exact matches

The cells used to build the organs could be cultured from stem cells taken from fat or bone - marrow tissue, he says: «We could engineer a blood vessel from your own cells.»

protected animals»); studies on in vitro systems (whole perfused organs, tissue slices, cell and tissue cultures, and subcellular fractions); and human studies (including estimations of occupational and environmental exposure, postmarketing surveillance, epidemiology, and the ethical and strictly controlled use of human volunteers).

The substance may be used for biomedical applications, ranging from cell culture and drug delivery to repairing and replacing tissue, organs and cartilage.

We have been using cell culture models and zebrafish as an assay to determine the effects of BMP on signaling, but we also want to be able to understand what the effect of the mutation is in the tissue and organ systems.

The resulting organoids could either be banked for future use or immediately fragmented and seeded into the «epithelial» channels of multiple Organ Chips where they were further matured by flowing specialized culture medium and applying mechanical stimulation to the channels to mimic physiological peristalsis - like motions.

To overcome these limitations, we used two - color flow cytometry to identify and select microvascular endothelial cells from primary cultures obtained from different organs of mice whose tissues harbor a temperature - sensitive SV40 large T antigen (H - 2Kb - tsA58 mice; ImmortoMice).

Pilot studies using a variety of strategies suggested that this goal could be best achieved by stimulating primary organ cell cultures with TNF - α (10 ng / ml) and targeting cells expressing the endothelial cell adhesion molecules E-selectin and VCAM - 1 through a FACS - driven strategy.

Consequently, levels of TGFβ were measured from the Optisol media used for the rabbit and human corneas in organ culture following wounding over time (Figure 5).

Chairs: Elena Aikawa, Brigham and Women's Hospital / Harvard Medical School and Anjelica Gonzalez, Yale University Christopher Hughes, University of California, Irvine Vascularized and perfused micro-tissues in culture and their use in drug screening Ying Zheng, University of Washington Engineering organ - specific microvasculature

Microphysiological systems (MPSs) are in vitro models that capture facets of in vivo organ function through use of specialized culture microenvironments, including 3D matrices and microperfusion.

It includes a new, streamlined chapter that combines the review of alternative techniques to animal experimentation such as the use of isolated organs, cell cultures, and computer simulations.