Sentences with phrase «using phosphorylation»

Every cell in the body uses phosphorylation, the process of adding a chemical tag to control a protein's function and fate, such as when it moves from one part of a cell to another or binds to other proteins.

Not exact matches

For example, cancer cells tend to use glycolysis in the cell cytoplasm in their metabolism, an ancient system, whereas healthy cells use oxidation - phosphorylation.

The research team used mass spectrometry to compare phosphorylation of proteins from mouse embryonic stem cells with fully functioning GSK - 3 to cells in which the gene encoding GSK - 3 had been deleted.

The turkey erythrocyte model system was used earlier to show that a covalent modification of the receptor (phosphorylation) is associated with this process.

Ueda's group performed experiments to show that phosphorylation activates WRKY2 by using mutants absent of SSP and replaced serine residues in WRKY2 with asparagine by base exchange, which mimics serine phosphorylation.

In this study, by using a systemic mtEF4 gene knockout mouse model, researchers found that mtEF4 knockout damages the oxidative phosphorylation function in germ cells of male mice, thus causing male sterility.

HIT exercise can have a positive effect on a person's maximum oxygen consumption (VO2max) and mitochondrial oxidative phosphorylation (OXPHOS) capacity, the pathway that cells use to metabolize energy.

«We realized that studying the lithium response could be used as a «molecular can - opener» to unravel the molecular pathway of this complex disorder, that turns out not to be caused by a defect in a gene, but rather by the posttranslational regulation (phosphorylation) of the product of a gene — in this case, CRMP2, an intracellular protein that regulates neural networks,» added Snyder.

This discovery provided the physiological basis for subsequent development of fMRI and caused researchers to reconsider the dogma that brain uses oxidative phosphorylation exclusively to fuel its functional activities.

We have used systematic molecular bioengineering of the meningococcal LPS through deletion of enzymes involved in LPS biosynthesis in combination with induction of LPS modifying enzymes (for example the deacylase PagL) to yield a variety of novel LPS mutant strains with changes in both lipid A acylation and phosphorylation.

Also called OXPHOS Complexes (Oxidative Phosphorylation) that make redox NADPH be used as electron donor through ETC and allow mitochondria to produce cell energy ATP.

Instead of having to express proteins when they are needed and degrade them when they are not, phosphorylation can be used as a switch to turn a protein on or off, so to speak.

Quantification of rapid Myosin regulatory light chain phosphorylation using high - throughput in - cell Western assays: comparison to Western immunoblots.

Modifications for Mitochondrial MEDEVAC However, when they then went on to attempt to use their system to introduce WT mitochondrial tRNAs into MELAS and MERRF cybrids — and thus rescue oxidative phosphorylation (OXPHOS)-- Teitell's group encountered a series of hurdles requiring additional modification of their constructs.

For this, the cells use the tricarboxylic acid (TCA) cycle, also known as the Krebs cycle and as oxidative phosphorylation.

We also show that inhibition of mTOR with rapamycin, as well as silencing mTOR gene product expression using mTOR - specific small interfering RNA, decreased phosphorylation of mTOR signaling proteins and induced cell cycle arrest and apoptosis in ALK + ALCL cells.

But if we ever want to use this class of compound to treat patients, we would want to know what else happens in the cell when you suppress tau phosphorylation by inhibiting GSK - 3.»

Phosphorylation of signaling proteins was investigated using the following phospho - specific antibodies from Cell Signaling Technology: anti-pY1034 / 1035 JAK1 (# 3331S), anti-pY1007 / 1008 JAK2 (# 3771S), anti-pY705 STAT3 (# 9131S), anti-pY694 STAT5 (# 9351S) and anti-p44 / 42 MAPK (# 9101L).

Analysis was carried out using purified c - ABL and the substrate peptide, EAIYAAPFAKKK, in association with the MRC Unit on Phosphorylation and Ubiquitination, University of Dundee, Scotland.

Phosphorylation of JAK1 and STAT5 was detected using specific anti-p1034 / 35 JAK1 and anti-pY694 STAT5.

Phosphorylation of JAK1 and STAT5 was detected using specific anti-pY1034 / 35 JAK1 and anti-pY694 STAT5.

First, we will use our unique DNA - and chromatin - templated in vitro transcription systems to investigate the direct role of p53 serine18 phosphorylation in sestrin gene activation through modulation of p53 - coactivator interactions.

Having established the importance of the miR - 181a target genes, Smad1 and Smad5, in BMP / GDF ligand - induced neurite growth, we next determined whether miR - 181a - modulated Smad phosphorylation / activation and neurite growth in SH - SY5Y cells, which are a widely used cell line model of human mDA neurons which also express the miR - 181a targets, BMPR2 and Smad1 / 5 [18,38].

We have identified tyrosine and serine / threonine phosphorylation sites of Eph receptors and ephrins using mass spectrometry and investigated the signaling role of these phosphorylation sites.

Phosphorylation sites were predicted using Scansite 2.0 (http://scansite.mit.edu; [25]-RRB- set on high stringency.

Our new study uses transcriptomics of cell fragments of the ciliate Stentor to reveal the importance of protein phosphorylation, microtubule - based processes and genes involved in the cell cycle for cellular regeneration.

The first task was to identify several specific amino acid sites in the region FUS uses to bind with other proteins where the natural enzyme DNA - dependent protein kinase (DNA - PK) causes phosphorylation.

«No one has shown that you can use charge, and phosphorylation as a way to get charge, to disrupt these ALS - associated protein aggregates,» said co-corresponding author Nicolas Fawzi, an assistant professor in the Department of Molecular Pharmacology, Physiology and Biotechnology at Brown University.

The body uses molecular oxygen to produce energy via oxidative phosphorylation in the mitochondria.

It generates energy in the form of ATP using oxidative phosphorylation (OxPhos).

METHODS: We treated 3T3 - L1 adipocytes with 2.5 mmol / l R (+) alpha - lipoic acid for 2 to 60 min, followed by assays of: 2 - deoxyglucose uptake; glucose transporter 1 and 4 (GLUT1 and GLUT4) subcellular localization; tyrosine phosphorylation of the insulin receptor or of the insulin receptor substrate - 1 in cell lysates; association of phosphatidylinositol 3 - kinase activity with immunoprecipitates of proteins containing phosphotyrosine or of insulin receptor substrate - 1 using a in vitro kinase assay; association of the p85 subunit of phosphatidylinositol 3 - kinase with phosphotyrosine proteins or with insulin receptor substrate - 1; and in vitro activity of immunoprecipitated Akt1.