Sentences with phrase «using surface markers»

Most of these CSCs have been identified using surface markers that identify their corresponding normal tissue stem and progenitor cells, thus reinforcing the resemblance of tumor development to normal organogenesis.

To characterize activation of immune cells, measurement of intracellular signaling using phospho - specific antibodies combined with immune phenotyping using surface markers is ideal.

Not exact matches

The company wanted to use it as a diagnostic marker for disease: If synthetic versions of antibodies could be tagged with aequorin in the lab, then whenever they matched up with an antigen (or surface protein) of a specific pathogen in blood or tissue, the sample would glow.

Several studies have used cell - surface markers — proteins found on the outer membranes of tumor cells — to identify glioblastoma stem cells; but the specific markers used have been controversial and can not reflect molecular processes going on within tumor cells.

The use of cell surface markers to isolate specific cell populations is one common method for separating cells; however, isolating live cells based on their RNA expression is a powerful new way enabling the study of small cell niches in nongenetically modified animal models and human tissue.

Once they were able to isolate skeletal muscle cells using the newly identified surface markers, the research team matured those cells in the lab to create dystrophin - producing muscle fibers.

Using the ERBB3 and NGFR surface markers, the skeletal muscle cells were isolated and then injected into mice at the same time a TGF Beta inhibitor was administered.

Skeletal muscle cells isolated using the ERBB3 and NGFR surface markers (right) restore human dystrophin (green) after transplantation significantly greater than previous methods (left).

The group was then able to sort the cells using markers expressed on the cell surface, to help pull out successfully edited cells for research, and eventually for therapeutic use.

In breast cancer, CSCs or tumor - initiating cells were first identified by using a combination of cell surface markers, CD24 − / CD44 + / ESA (EpCAM) + (2).

The authors used the cell surface marker podoplanin as a handle for isolating the progenitor cells from bone marrow.

[B and E] Brain leukocytes were percoll purified from the remaining brain and meningeal homogenate, stained for leukocyte surface markers CD4, CD8, CD19, CD11b, and Ly6G, and assayed using flow cytometry.

Purified cells were surface stained for a variety of leukocyte markers and analyzed using flow cytometry.

Using single water molecules as atomic markers, the team successfully identified the atomic species of this surface; result that was additionally confirmed by the comparison of simultaneous AFM and STM measurements with the outcomes of first - principles calculations.

[B] Brain leukocytes were percoll purified from the remaining brain and meningeal homogenate, stained for surface markers CD4, CD8, CD19, CD11b, and Ly6G, and assayed using flow cytometry.

As the studied chemical reactions occur very rapidly — in less than a second — and the ALS technique can take about 20 minutes to scan a single spot on a particle, the researchers used a layer of chemically active molecules, which were attached to the surface of the particle, as markers for the catalytic reactivity.

Positive clones were then differentiated using defined serum - free conditions outlined in Ng, et al [7], which overall gave rise to a CD45 + CD56 + CD117 − CD94 + population of cytotoxic NK cells [8] expressing the CD4 construct and other surface markers in a similar manner to peripheral blood NK cells.

The percent area covered by blood platelets over the luminal surface of untreated or treated ePTFE tubings was calculated by calculating the percent area covered by CD41 (platelets marker) using binary image processing on Zeiss LSM Image Examiner software.

We derived a fibroblast line from a skin biopsy from a healthy adult male (HUF1)(Figure 1A) and used immunocytochemistry to characterize the expression of cell surface markers commonly expressed on pluripotent stem cells (Figure 1B, C and D).

Our laboratory used two monoclonal antibodies, GCTM - 2 and TG30, recognizing the cell surface proteoglycan characteristic of primate ES cells and CD9 respectively, to fractionate human ES cell populations into four compartments according to their relative levels of expression of both markers [14].

Following the included instructions, we used a grinder to clean the contact surface on the spindles; we marked our welds in paint marker, then proceeded to weld on the gussets.

ITRI also mentions that normal solvent marker pens can also be used to write on its surface.

The samples were stained and examined histologically, and cell surface markers investigated using immunohistochemistry and flow cytometry.

Along with your basic scuba equipment, you'll learn to use various surface marker buoys and floats with lines and reels.

Metallic or permanent markers can be used to draw on top of the acetate surface.

A contemporary idea of beauty is challenged and dismantled by the artist's use of cheap materials and direct interventions on the surface of works with black markers, acts of apparent sabotage that demystify the highly - constructed visuals of the mass media.

PP: For the paintings, yes I use medium tip marker pen — not the large ones — and use silver and black, which was what we used when we went out (doing graffiti) which meant we where covered for both dark and light surfaces.

For a long time I used black ink on paper, then I discovered coloured markers and that opened up the process because then I could start drawing with colour as a means to create composition and think about how shapes and colours interact on the initial drawing surface.