The approach to target validation
utilizes RNAi, CRISPR, and ORF
expression platforms to genetically perturb candidate target gene
expression in relevant cancer cell lines, and then profile the resulting phenotypic
changes with regard to their effect on various biochemical pathways.
By
utilizing paired genome - wide high - throughput DNA sequencing (DNA - seq) with RNA - seq, we found that gene dosage, at the level of individual genes or chromosomal «somy» (a general term covering disomy, trisomy, tetrasomy, etc.), accounted for greater than 85 % of total gene
expression variation
in genes with a 2-fold or greater
change in expression.
- Link doesn't have much of a character
in this game - they wanted players to relate to Link and play as themselves - Link's face doesn't have much
expression in this game, and that was done on purpose - for Zelda games, the devs always try to make the player feel like Link - that was pushed even moreso for this entry - everytime a Zelda releases, Aonuma watches fan feedback and considers that for the future - the items Link uses to solve are always going to be important - regardless of the tech that Nintendo provides, exploration
in the series is important - Link's growth through who he meets is also an important element of the franchise - Aonuma thought about Zelda freedom when considering Breath of the Wild, and was brought back to the days of the original Zelda - this was a big point of inspiration for Breath of the Wild - with Ocarina of Time 3D, Nintendo wanted to really
utilize the 3D and had a desire to let players enjoy Ocarina of Time outside - the goal with A Link to the Past was creating more depth - Aonuma used to think that getting lost
in a 3D world wasn't okay - this lead Aonuma to putting
in tons of hints and guiding players - he has since
changed that idea and convinced himself that it's okay to get lost - the first two years of development the devs created a game where just running around was fun - climbing is very important, because players can climb high and see destinations they want to visit - you can place your own markers to decide where you want to go - when the devs create a Zelda title, they consider not using the name Link - since they don't have anything else to go with, they choose Link - the name Link doesn't hold much value, since the player is the one creating the journey - when Nintendo first created Link, they wanted a character that is literally the link between people - that is why his name is Link - to think that the Zelda series has been around so long that there are all sorts of urban legends and myths makes Miyamoto really happy