Sentences with phrase «vector transduction»

Significant additional preclinical and clinical studies addressing vector transduction efficacy, biodistribution, and toxicity are required before a human in utero gene transfer protocol can proceed.

The general approach is to develop new vector systems and delivery methods, test them in the appropriate animal models, uncover the mechanisms involved in vector transduction, and use the most promising approaches in clinical trials.

Not exact matches

At the end of the project, the transduction efficiency of the newly made retroviral vectors was tested in hematopoietic cells, and here my «old» techniques came in handy.

OMC also aims at potentiating the existing Conditional Mutagenesis Facility by implementing novel approaches to attain tissue -, stage - and site - specific transgene expression in mouse mutant strains, mainly through lentiviral vector - mediated transduction of blastocysts.

The use of viral vectors in research is beneficial for a number of reasons, including but not limited to: helping to get difficult - to - deliver DNA into mammalian cells, increasing the efficiency of gene transduction, allowing for control over which cells are infected through viral pseudotyping, and ease of vector cloning and modification.

Systemic FIV vector administration: transduction of CNS immune cells and Purkinje neurons.

Transduction of the mammary epithelium with adenovirus vectors in vivo.

Augmentation of adenovirus 5 vector - mediated gene transduction under physiological pH conditions by a chitosan / NaHCO3 solution.

In vivo gene delivery and stable transduction of nondividing cells by a lentiviral vector.

Functional correction of T cells derived from patients with the Wiskott - Aldrich syndrome (WAS) by transduction with an oncoretroviral vector encoding the WAS protein.

Kim Y - S, Wielgosz M, Hargrove P, Kepes S, Gray J, Persons D, Nienhuis A. Transduction of Human Primitive Repopulating Hematopoietic Cells with Lentiviral Vectors Pseudotyped with Various Envelope Proteins.

Tags for this Online Resume: Lentiviral Retroviral shRNA RNA knockdown screens, Flow Cytometry, RTPCR, Immunopreciptations, Reporter assays, Cell based Assays, ELISA, Migration, Stable and Transient Transfections, Genomic and Proteomic Studies, Cloning, Transient siRNA knockdowns, Phenotyping Mammalian cell lines, Gene Overexpression, Subcloning to expression Vectors, Construct Stable Inducible Cell lines, Protein Kinase Assay, Pulse Chase Experiments, Transfection, Transduction, Transformation, Polyoma virus DNA tumor virus replication, Research Scientist, Cell Biology Protein Biochemistry Genetics Molecular Bioliogy Oncology Virology, FACS

Tags for this Online Resume: Ecl (Control Lang), Purchasing, Software, ARIA, Injections, Intelligent Call Management, Research, Transcription, Genotyping, Western blot, viral vectors, DNA cloning, RNA extraction, RNA syntehesis, confocal microscopy, cell culture, transfection, transduction, western blot, PCR, RT - qPCR, Next Generation DNA sequencing, ion torrent, gromacs, ICM, Schrodinger Tools, NCBI software, DIVA, FlowJaw, Flow Cytometry, Cell Sorting, Aria II, Aria III, XCalibur, TaqMan, electrophoresis, Sanger DNA sequencing, FCS Express, cell patterning, ELISA, immunoassay, antibody, HPLC, his - tag, flag - tag, flash - tag, chromatography, stem cells, DNA extraction, CRISPR, cas9, plasmid, lentivirus, CAR - technology, MLV, Chimeric Antigen Receptors, Jurkat, U87, HeLa cells