Sentences with phrase «viable cells from»

Complete phenotyping of the mouse immune system by polychromatic and mass cytometry (CYTOF), thanks to a set of standardised protocols enabling isolation of viable cells from lymphoid and non-lymphoid organs (lung, skin, intestine,...) for labelling using complex ranges of antibodies whose compatibility allows the simultaneous registration of 50 quantitative parameters at least (size, structure, specific antibodies and cell viability).

Not exact matches

Human parthenogenetic embryos are not viable — they run into developmental snags and can not give rise to a person — but the stem cells derived from these embryos could still have research or therapeutic value.

The causes of infertility, which affects around 10 % of couples, are often unknown, but may in some cases result from the body's inability to produce viable gametes — also known as sperm and egg cells.

If Kazufumi Goto and Akira Iritani come back to Japan with sperm and viable DNA from some other cell of a mammoth, they'll face a choice: they can try to create either a hybrid or a clone.

To turn this into something that could one day be a viable therapy for people, the team took stomach stem cells from diabetic mice, engineered them with the same genes and grew mini-organs.

By flipping on three key genes in cells from the lower stomach, scientists may have engineered a viable replacement for those beta cells.

They went an extra step and chemically removed cells that did not have intact membranes, increasing the likelihood that the DNA they isolated came from viable bacteria and fungi and not just remnants of dead bugs.

For the most critically endangered species there should, I believe, be far more effort to preserve viable cell cultures from as many different individuals as possible.

Six years ago, President Bush limited federally funded research to about 20 viable lines of cells that had been extracted from embryos prior to August 9, 2001.

In the 1960s, he transplanted nuclei from frog intestine and skin cells into frog egg cells and created a viable animal.

If that turns out to be viable, you might end up with people having their own cell line almost as a matter of course 20 years from now.

If the cells can be fertilized and develop into viable embryos, and if human ES cells turn out to have similar powers, such cells could allow researchers to get around some of the expense and ethical questions that arise from using donated eggs for therapeutic cloning experiments.

Another Chinese team, from Guangzhou Medical University, in March became the first to report repairing disease - causing mutations in viable embryos, but some still contained a patchy mix of edited cells — a phenomenon called mosaicism.

However, plerixafor, which has not been reported to have adverse immunologic consequences resulting from inhibiting CXCR4 function in mature CD4 + T cells, provides proof of principle that inhibiting CXCR4 in mature CD4 + T cells may prove to be safe and viable [10], [53].

However, homozygous XPDXP (XPDR683W) and hemizygous XPDXPCS (XPDG602D) human cells are known to be highly sensitive to UV [19,25], as are cells from a homozygous viable XpdXPCS / XPCS (XPDG602D / G602D) mouse model (Figure 4A, dotted line)[23].

For the past three years, McDevitt and his team have been working to culture viable human V2a interneurons from pluripotent stem cells.

The Georgetown team's preliminary data demonstrates that they collected sequenced DNA over 68,000 base pairs long from 10,000 year - old microbial samples, meaning that the DNA came from viable cells.

2) Cloning with cells from cryopreserved tissue of a recently extinct animal can generate viable eggs.

At a time when scientists struggled to maintain viable cell cultures, cells derived from her biopsy proved uniquely able to survive and proliferate in culture — in fact, early HeLa cultures doubled every 24 hours!

Generating mature and viable heart muscle cells from human or other animal stem cells has proven difficult for biologists.

Immunofluorescence analysis of the two populations, following overnight adherence to exclude analysis of non-viable cells, revealed that > 97 % of the SSEA3 - positive population expressed detectable SSEA3 / 488 fluorescence and 0 % of the SSEA3 - negative population expressed detectable SSEA3 / 488 fluorescence (Figure 2C), demonstrating that the fluorescence activated cell sorting process can purify viable subpopulations of cells from a heterogeneous somatic population.

We suggest correct identification of the origin of these cells (using human specific markers, which define cell membranes) is essential in order to distinguish viable donor cells from host inflammatory cells which have engulfed transplanted cells.

After 3 h, media was aspirated from all wells, leaving purple formazan crystals in those wells with viable cells.

Flow cytometry allows researchers to quantify and isolate rare viable cell subsets from heterogenous populations in single - cell suspensions based on differential expression of surface antigens.

Another major difference from the rest of the market is the number of viable cells per unit.

A measure of viable bacterial cells in a colony representing an aggregate of those cells derived from a single originating bacteria.

The Chinese services are free because the only viable means to charge is via cell phone billing, and the government just reduced the amount that can be charged to 10RMB a month, from 20RMB a month.

Unlike the Naruto: Ultimate Ninja Storm series which is more and more a mess of technical, balance and gameplay issues these days, Brave Soldiers delivers what is a nice, franchise - based fighting game, at first, i was expecting a simple fighting game with some button mashing, however, the game proved me wrong and i fell in love, the combo system, while easy, is a lot more deep than the one in the Naruto games, with all of the characters having two special attacks, two «burst attacks», a knock - away and a launcher respectively, a throw and an ultimate attack (called a «Big Bang Attack»), every character also has an universal dodge - action that sends them behind their enemies while spending one cosmo bar, making bar management that much precious and shielding you from a half - a-hour combo, unlike in the NUNS series, the fighting and the characters are nicely balanced, with every character being fun to play and viable at the same time, the game runs smoothly without frame - rate issues and the cell - shaded graphics, character models, arenas and effects alike are nice to the eye, battles are divided into rounds, with all the tiny nice stuff like character introductions and outros being intact (fun fact: the characters will even comment on their score after the battle), the game also features an awakening system, called the «Seventh Sense» awakening, unlike the NUNS awakening system which became severely unbalanced in the later game, every character simply gains a damage / defense boost, with the conditions being the same for all characters, eliminating situations when one character can use awakening at almost any point in the battle, or one awakening being drastically stronger than the other, the game has a story mode with three story arcs used to unlock characters, a collection mode, tournament modes, a survival mode, a series of special versus modes and online battle modes.

How can Paul Nurse know, for example, Andrew Montford's position on whether stem cells useful for medical research should be generated from foetuses that are consequently not allowed to develop into viable human beings or from adults who do not suffer as a result?

Hydrogen fuel cell cars are the right answer to our current plight but why in the world is Honda not pursuing extracting the hydrogen from water, when that technology is arleady available and viable?