However, due to limitations in the size of constructs that can be efficiently inserted using current CRISPR / Cas technologies, complex projects including, sgRNA expression cassettes, promoters, polyadenylation, and fluorescent protein sequence can not all be incorporated into a single
viral expression vector that also includes Cas9 cDNA.
Once again, changing Tat levels was sufficient to turn the virus on or off, and relaxing or activating the host cell did not
affect viral expression.
Some highlights of the 2nd edition eBook include: new chapters on genome engineering, cloning, and plasmid tags, addtional content added to the «Plasmids that Glow» and «
Viral Expression Vectors» chapters, and more pictures and pro tips.
These differentiated cells are then infected with HIV - 1 and
viral expression is monitored.
In virtually
all viral expression systems employed by scientists, non-essential components are stripped away and the remaining native genes are spread over multiple plasmids to ensure safety.