Some highlights of the 2nd edition eBook include: new chapters on genome engineering, cloning, and plasmid tags, addtional content added to the «Plasmids that Glow» and «
Viral Expression Vectors» chapters, and more pictures and pro tips.
Not exact matches
Overexpression of wild - type ACVR1 in chick micromass cultures resulted in
expression levels of marker genes that are comparable to those of the empty
viral vector, while cells overexpressing R206H ACVR1 showed upregulation of collagen type II and aggrecan.
Remarkably, the relative
expression levels of all marker genes were only slightly decreased when R206H ACVR1 and Q207D caACVR1 cultures were cotransfected with Noggin, whereas cells expressing the wild - type ACVR1 or empty
viral vector showed clear downregulation of all analyzed marker genes for chondrogenesis.
While
expression levels of all marker genes remained almost unchanged in wild - type ACVR1 cultures compared with the empty
viral vector, the
expression profile induced by caACVR1 Q207D displayed evidence of advanced chondrogenic differentiation as shown by decreased levels of the early differentiation stage marker collagen type II together with enhanced
expression of the later - stage markers Ihh and collagen type X. Indian hedgehog has been shown previously to be induced by BMP signaling transmitted via the ACVR1 receptor (9).
Recombinant: Vaccine manufactured through gene cloning (plasmid expressed) or by recombining selected DNA from a pathogenic organism with DNA from another virus (
viral vectored), leading to the
expression, following inoculation, of only essential antigens required to immunize.