Here we describe a pipeline for the functional annotation of
viral metagenomic sequence data.
Not exact matches
Using nanopore
sequencing,
metagenomic detection of
viral pathogens directly from clinical samples was performed within an unprecedented < 6 hours sample - to - answer turnaround time and in a timeframe amenable for actionable clinical and public health diagnostics.
We have in - house computational pipelines built for
metagenomic viral discovery, RNASeq quantification, exome
sequencing and whole genome
sequencing for variants detection using GATK, Affymetrix & Illumina expression array and SNP chips, GWAS, digital PCR analysis, mass spectrometry and biomarker discovery.
Genome
sequencing has become a powerful tool for studying emerging infectious diseases; however, genome
sequencing directly from clinical samples without isolation remains challenging for viruses such as Zika, where
metagenomic sequencing methods may generate insufficient numbers of
viral reads.