«We saw clearance of
the virus at concentrations of BX795 that were five times lower than concentrations of antiviral compounds in available nucleoside analogs,» Shukla said.
They were able to show that treatment of the cells with three drugs, called obatoclax, saliphenylhalamide and gemcitabine, prevented synthesis of viral building blocks and production of new
viruses at concentrations that are not toxic to cells.
Not exact matches
This synergy allows the drugs to be used
at lower
concentrations, thereby reducing toxicity, and could «increase the chances that only the
virus is reactivated,» Verdin says.
The LD50 for all the
viruses was determined by in vivo infection
at various
virus concentrations, according to the method of Reed and Muench.
To perform the
virus neutralization assay, 55 µL of viral supernatant
at a
concentration of 100 TCID50 and 55 µL of serum (starting
at a 1 ∶ 8 dilution) were mixed and incubated for 1 hour
at 37 °C.
Patient 1's blood had a very high
concentration of
virus,
at 200 million particles per milliliter.
Another investigator reviewed medical records and recorded signalment, environment, previous disease, diseases evident
at time of radiography, FeLV vaccination and infection status, feline immunodeficiency
virus serologic status, serum creatinine
concentration, serum globulin
concentration, and any other important findings.