Not exact matches
They amplified the RNA with the polymerase chain reaction, which provided enough to
sequence parts of the
virus's genetic
code.
Each
virus contained genetic
sequences coding for one of two proteins: miR24, a cancer - fighting antibody, or human interferon beta - 1a, a protein with antiviral properties.
The vaccinia
virus 13L early / intermediate promoter is cloned upstream of the nef
coding sequences.
Genomic
sequencing of the isolated
virus revealed that it is closely related to Tembusu
virus (a mosquito - borne Ntaya group flavivirus), with 87 — 91 % nucleotide identity of the partial E (envelope) proteins to that of Tembusu
virus and 72 % of the entire genome
coding sequence with Bagaza
virus, the most closely related flavivirus with an entirely
sequenced genome.
Sequencing and BLAST analysis recealed a 87 — 91 % / 87 % nucleotide identity with a partial
coding sequence from the Tembusu / Sitiawan
virus E gene (encoding the envelope protein)(Fig. 7A).
Next generation
sequencing generates a huge amount of genetic
code data on all the
viruses in one animal.