Chen and her colleague Guan - Zhu Han, an evolutionary biologist based at Nanjing Normal University, Nanjing, China, evaluated all dengue
virus sequences from China available in the public database GenBank — about 1,000 - 4,000 samples each for the four dengue serotypes (DENV 1 - 4), for almost 10,000 total.
Not exact matches
Here's how it works: When a bacterium encounters DNA
from a
virus, it makes a strand of RNA, a molecular cousin to DNA, that matches the
sequence of the viral DNA, known as a guide RNA.
Genetic
sequencing by IAH scientists has, however, confirmed the two outbreaks sprung
from the same
virus, suggesting the culls and biosecurity measures failed to contain foot and mouth.
Many scientists also argue that destroying the stocks would do little to protect the world
from smallpox, because it is now possible to recreate the
virus from its genome, which was
sequenced in 1994.
An analysis of the HPV16 genome
from 5,570 human cell and tissue samples revealed that the
virus actually consists of thousands of unique genomes, such that infected women living in the same region often have different HPV16
sequences and variable risks to cancer.
As is typical of newly isolated HIV
viruses from Africa, the
sequences did not match any HIV
sequence then available on GenBank, the online database of genetic
sequences maintained by the U.S. National Institutes of Health.
CDC, racing to
sequence the
virus from those infected, said it's seeing «slight changes» in its genetic makeup, but, said Besser, agency scientists don't know what that means.
Data
from in vivo mouse models indicate that delivery of the DMAb
sequence for the influenza A-targeted monoclonal antibody protected against lethal doses of two very different, clinically relevant influenza A
viruses.
«Our genomes are packed with
sequences derived
from viruses that were inserted many thousands of years ago, and these
virus - like
sequences can move around, causing genetic mutations and contributing to the evolution of our genomes.
HPV is detected using a DNA analysis that looks for characteristic
sequences from the 13 high risk strains of the
virus in the cervical cell sample.
We also
sequenced 35 samples
from suspected EVD cases that tested negative for EBOV; genomic analysis identified other known pathogens, including Lassa
virus, HIV - 1, enterovirus A, and malaria parasites (fig.
The authors analyzed changes in the viral
sequence and conclude that the current outbreak probably resulted
from the spread of the
virus from central Africa in the past decade.
We used massively parallel viral
sequencing to understand how and when EBOV entered human populations in the 2014 West African outbreak, whether the outbreak is continuing to be fed by new transmissions
from its natural reservoir, and how the
virus changed, both before and after its recent jump to humans.
We
sequenced 99 Ebola
virus genomes
from 78 patients in Sierra Leone to ~ 2000 × coverage.
Unlike the previous studies that focused on certain species or a particular RNA
virus, Skalka went broad: She and her colleagues surveyed every vertebrate genome available, 48 in all, and looked for hints of 5666 RNA viral
sequences from 38 known families and nine genera that were unclassified.
However, they identified genetic
sequences matching
viruses that infect dogs, chickens, and pigs, suggesting the possibility that some of the
viruses had crossed over
from other species.
The researchers compared the HSV - 1 and HSV - 2 gene
sequences to the family tree of simplex
viruses from eight monkey and ape host species.
When the researchers expanded their search to include all the data
from the Human Microbiome Project, a large - scale project to
sequence the DNA of all the microbes that live in and on our bodies, they found that the same
virus was present in 73 per cent of all 466 human faecal samples.
A team of researchers
from the United Kingdom has obtained the first complete genome
sequence of Zika
virus that was isolated
from a semen sample.
Quintana - Murci and colleagues used RNA -
sequencing to characterize the way that immune cells, known as primary monocytes, derived
from 200 people of self - reported African or European ancestry would respond to attack by a bacteria or a
virus.
In rapid - fire
sequence he jumps
from scientific minutiae to grand political comparisons (
viruses, bacteria, oncogenes, even researchers who study these entities can be transformed into Goebbels or «good Germans»), and then he might toss in an entirely new idea before returning to his original topic — all within seconds.
We analyzed 7.2 terabases of metagenomic data
from 243 Tara Oceans samples
from 68 locations in epipelagic and mesopelagic waters across the globe to generate an ocean microbial reference gene catalog with > 40 million nonredundant, mostly novel
sequences from viruses, prokaryotes, and picoeukaryotes.
The successful isolation of Zika
virus from this semen sample allowed the investigators to
sequence the
virus, resulting in the first genome of Zika
virus isolated
from semen.
Using a specially designed computational tool as a lure, scientists have netted the genomic
sequences of almost 12,500 previously uncharacterized
viruses from public databases.
Genome
sequencing of specimens
from both Canada and Mexico will help us to understand if the
virus has mutated which may help us to explain the increased severity of the cases in Mexico.
They extracted and
sequenced RNA
from 41 strains of HIV as well as 61 strains of hepatitis C
virus infecting the children.
The finding doubles the number of recognized
virus genera — a biological classification one step up
from species — and increases the number of
sequenced virus genomes available for study almost tenfold.
But the genome
sequences revealed in this study suggest that there are many more prophage - like
viruses that are different in one important respect: Their genome remains separate
from their microbial hosts» genome.
Lipkin then used a rapid genome -
sequencing technique to identify RNA
from a variety of bacteria, fungi, and
viruses.
The CRISPR - Cas system protects bacteria
from invaders such as
viruses by creating small strands of RNA called CRISPR RNAs, which match DNA
sequences specific to a given invader.
And a new prototype device can display a fluorescent letter in the presence of nucleic acid
sequences from the Ebola
virus or the closely - related Marburg
virus: «E» for Ebola or «M» for Marburg.
These results appear only now, months after the peak of the outbreak, because
sequencing Zika
virus has proved to be challenging, particularly directly
from patient samples.
To narrow down this time window, Whitfield studied
sequences of three genes
from 28 species of wasps — some
virus - carriers and some not — and used differences among the species to build a family tree.
With the aid of advanced gene
sequencing, comparative DNA analysis, and endless cross-referencing of the genomes of organisms
from the three — or perhaps four — domains of life, a fuller concept of
viruses and their role in evolution has begun to coalesce.
In the past two years, genetic
sequence information of the flu -
virus protein hemagglutinin
from only two influenza strains
from India has been deposited into publicly available influenza databases, making it difficult to determine exactly which strain is causing the new outbreak, and how it differs
from previous strains.
We are nowhere near being able to predict
from a flu
virus's genetic
sequence whether it will be transmissible or virulent, and our surveillance of flu strains in the wild is extremely limited.
But this month, researchers reported that the instruments passed an important field test, conducting on - the - spot
sequencing of
viruses isolated
from patients during last year's Ebola epidemic in West Africa.
«Amazingly fast, cheap genome
sequencing: Zika
virus mosquito genome assembled
from scratch.»
Thirty - one years ago, Gould and Vrba suggested that repetitive DNA
sequences known as transposons, which originated
from viruses, might serve no direct function at first, but may be used to great advantage later on.
A multi-institutional team has
sequenced the genome of the mosquito that transmits the Zika
virus uisng a new way to
sequence the genome of an organism entirely
from scratch, dramatically cheaper and faster.
Glenn Browning at the University of Melbourne in Australia and colleagues
sequenced the
viruses and found that they arose when the European strain acquired genes
from the Australian strains.
From its
sequence, researchers already knew that the 1918 HA gene resembled that of avian flu
viruses.
Preliminary
sequencing of
virus isolates
from Reunion at the Pasteur Institute in Lyon, France, suggests that the
virus was imported
from East Africa, says Pasteur virologist Nathalie Pardigon.
When the team
sequenced these regions and compared them to those
from SFV strains isolated
from a wide variety of different species, they discovered that one person had most likely been infected with an SFV strain
from a gorilla, one with a mandrill strain, and the third with a
virus from a monkey species called De Brazza's guenon.
In response to growing criticism about the lack of access to influenza data, the Centers for Disease Control and Prevention (CDC) in Atlanta, Georgia, on 22 August made public the
sequence of more than 650 genes
from flu
viruses isolated in the United States.
Even so,
sequences of the
virus have been published just twice, in March of 2014 (using samples
from Guinea) and in June of 2014 (using samples
from Sierra Leone).
They had also
sequenced the
virus directly
from samples taken
from the patient and the camel — and those two were also 100 % identical.
The Saudi scientists said they had isolated a
virus from the patient as well as
from one of the camels, grown both samples in monkey kidney cells, and
sequenced their genomes, which turned out to be 100 % identical.
Together the pieces represented about 15 % of the camel
virus genome and these parts were almost identical to the genome
sequenced from the patient.
In nature, bacteria use DNA snippets
from viruses to form CRISPR
sequences to fight viral infections.