Sentences with phrase «visceral endoderm»

(D) Sections of EBs grown under the same conditions as in part (C), showing that the outer layer consists of visceral endoderm as marked by Gata6, FoxA2, and Sox17 immunostaining (shown as red).

Black arrows show the presence of LacZ positive cells in the visceral endoderm.

When V+S + cells were recombined with V − S + cells immediately following sorting, they formed normal EBs and the V+S + cells move preferentially to the outside to form the presumptive visceral endoderm.

Not exact matches

Most strikingly when introduced back into morulae or blastocysts, the V+S + population is not effective at contributing to the epiblast and can contribute to the extra-embryonic visceral and parietal endoderm, while the V − S + population generates high contribution chimeras.

That we have observed a direct contribution of ES cells to both visceral and parietal endoderm also has implications for canonical definitions of pluripotency.

However, cells derived from the early epiblast are only able to contribute to embryonic lineages and not those derived from the PrEn [18]--[20], while PrEn cells can only contribute to their own lineage by colonizing the visceral and mostly parietal endoderm in chimera experiments [20]--[22].

Interestingly when parietal endoderm is grafted next to epiblast, it becomes visceral and when VE is removed from epiblast it becomes parietal [58].

Interestingly, while the V+S + cells did not effectively contribute to the epiblast, V+S + ES cells were found in both the visceral and parietal endoderm (Figure 8A, Table 2), suggesting that their reduced ability to contribute to the epiblast may reflect a change in potency.

Moreover, as it has recently been shown that VE can contribute to the embryonic gut [55], the distinction between visceral and definitive endoderm begins to blur and the inability of ES cells to contribute to the VE becomes more puzzling.

The Venus positive population does not efficiently contribute to somatic lineages, appears at the outside of EBs, and has the capacity to colonize the visceral and parietal endoderm in chimeras.

The epiblast is the source of all embryonic tissue and the PrEn the source of both extra-embryonic endoderm lineages, visceral and parietal.