Sentences with phrase «vitro model system»

A breakthrough in understanding the mechanism of lamellipodial protrusion came from development of an in vitro model system, namely the rocketing movement of microbes and activated beads driven by actin comet tails (Cameron et al., 1999, 2000; Loisel et al., 1999; Theriot et al., 1994).

However, hiPS cell - derived hepatocytes are only useful as an in vitro model system and for gene editing applications if they recapitulate critical hepatocyte functions.

The rarity of latently infected cells and the lack of a marker for latent HIV - 1 infection necessitate the use of in vitro model systems for detailed studies of this process.

Not exact matches

«Chronic inflammation of the intestine is thought to be caused by abnormal interactions between gut microbes, intestinal epithelial cells and the immune system, but so far it has been impossible to determine how each of these factors contribute to the development of intestinal bowel disease,» said Hyun Jung Kim, Ph.D., former Wyss Technology Development Fellow and first author on the study, speaking about the limitations of conventional in vitro and animal models of bacterial overgrowth and inflammation of the intestines.

The in vitro model was used to extensively characterize the differences between neurons from different regions of the brain and to mimic the system's connectivity.

«With Teresa Woodruff's research using Draper's human organ system platform, we have a compelling demonstration of the importance of a microenvironment that permits cells to function in vitro as they would in vivo, and the power of being able to interconnect organ models on a platform and operate them in a stable and precise manner for weeks to months,» said Jeffrey T. Borenstein, a biomedical engineer at Draper.

«Investigators have been developing in - vitro liver models for 40 years, but all of those systems ignore the distinct patterns of metabolically active hepatocytes that exist within the liver sinusoid» says Martin Yarmush, MD, PhD, director of the MGH - CEM and the paper's senior author.

After testing the system both in vitro and in vivo in an animal model, Scott's team demonstrated they could administer a subcutaneous injection that slowly delivered nanomaterials to cells in lymph nodes for over a month in a controlled fashion.

«We need to follow up with functional studies, such as in animal models or at least in vitro systems, to pinpoint and validate the biological basis for selection.»

This murine model system offers a superior source of immortalized cell populations as many of the difficulties associated with the in vitro transfection process are eliminated (e.g., initial requirement for many cells, different sites of gene integration, multiple copy number; Ref.

In the Thinakaran lab, Pierre is now utilizing in vitro and in vivo models to understand BIN1's function in the nervous system in health and disease.

Concomitantly, the NpSRII / NpHtrII complex will serve as model system for development of new site - directed spin labeling EPR methods to elucidate the structure and conformational dynamics of membrane proteins and protein clusters in vitro and in vivo.

The resulting data are being combined in a systems biology approach with high - resolution clinical phenotyping and findings obtained with a large array of established and novel in vitro, ex vivo and in vivo disease models to identify disease - associated genetic variants, disease - defining molecular signatures, and potential targets for therapeutic intervention.

The Cancer, Ageing and Somatic Mutation Programme encompasses three Projects that respectively cover the genomics of human cancers; functional analysis of the cancer genome using a range of in vitro and in vivo model systems; and the characterisation of somatic mutations in development and adult homeostasis in health and disease.

This approach is now being used to derive embryonic stem cells from a variety of strains including disease models from which in vitro tools are in demand, including mouse models of Alzheimer's disease, cytogenetic disorders (Turner's syndrome and Down syndrome), and tool strains for systems genetics.

Current in vitro hematopoiesis models fail to demonstrate the cellular diversity and complex functions of living bone marrow; hence, most translational studies relevant to the hematologic system are conducted in live animals.

For understanding the biology of gene - gene, gene - drug and gene - microenvironment interactions, a considerably broader range of in vitro and in vivo model systems is required — we are generating 1,000 organoid cultures from human cancers, characterising their genomes, functional dependencies and drug response, and we are expanding our in vivo models to study the interface between cancer and the immune system and microenvironment.

Our lab uses a variety of model systems, such as the nematode C. elegans, as well as in vitro culture systems, to address fundamental questions in cell cycle control during development and tissue formation.

We will mine and exploit information from studies and screens in model organisms, but our primary focus is on the paradigmatic mammalian stem cell systems - haematopoiesis and epithelia in vivo, and embryonic and neural stem cells in vitro.

Moreover, we were able to recapitulate toxicity findings from the clinic that were not previously observed in other in vitro systems or predicted by animal models.

Microphysiological systems (MPSs) are in vitro models that capture facets of in vivo organ function through use of specialized culture microenvironments, including 3D matrices and microperfusion.

As a model for investigation of the other major protrusive organelle, the filopodium, we developed in vitro systems for producing filopodia - like bundles (Vignjevic et al., 2003), one of which uses cytoplasmic extracts and another that reconstitutes like - like bundles from purified proteins.

Future models, it seems, will come in many varieties, ranging from several kinds of animals to in vitro systems to computers, with each approach building on the others.

These findings have identified an alternative source of replacement tissue for use in human retinal cellular therapies, and provide a new in vitro cellular model system in which to study RPE diseases affecting human patients.

To demonstrate Amrose functionality in an established higher model system, Amrose v0 — v4 variants were cloned into the pCS2 + vector system to synthesize mRNA by in vitro transcription, which was used for injection into one - cell stage zebrafish embryos.

in the short - term, further understand the molecular pathways involved in the process of oncogenic transformation in human hemopathies, using fine structural in vivo analysis of clinical materials, combined with the development of functional in vitro experimental systems and in vivo models of genetically modified mice;

Towards that goal, the Foundation invested heavily in essential drug discovery assets like animal models and in vitro systems to screen existing drugs and compound libraries.

-- Rifaximin modulates the colonic microbiota of patients with Crohn's disease: an in vitro approach using a continuous culture colonic model system.

Although canine models of various cancers garner increasing interest as in vitro systems suitable for studies of basic biology and drug development, the fact that breed specificity is observed for many types of cancer has excited geneticists who are anxious to localize susceptibility genes that have proved intractable in human families and populations.

I would like to work with in vitro systems with the goal of transfering the results into in vivo models and eventual human testing.