Future investigations using specialized, high - resolution in
vivo imaging tools, such as optical coherence tomography and scanning laser ophthalmoscopy, will permit a much more detailed investigation into the source of the autofluorescence.
To apply in
vivo imaging tools for non-invasive monitoring of the survival and growth of the grafted cells, as well as tools to reveal adverse immune / inflammatory reactions to the graft;
Although traditional in
vivo imaging tools, such as widefield and confocal microscopy, and newer ones, such as light - sheet microscopy, can image in three dimensions, they sacrifice substantial spatiotemporal resolution to do so and, even then, can often be used for only very limited durations before altering the physiological state of the specimen.
Not exact matches
The speed, noninvasiveness, and high spatial resolution of this approach make it a promising
tool for in
vivo 3D
imaging of fast dynamic processes in cells and embryos, as shown here in five surrounding examples.
IDMIT will contribute 1) To the development and validation of assays based on flow cytometry and mass cytometry for the evaluation of immune responses in humans and animal models; these
tools will be particularly relevant for the identification of signatures of vaccine efficacy; 2) To the animal model platform, in particularly by providing access to NHP models and to new technologies for in
vivo imaging infections and host responses; 3) To networking activities, in particular by organising a workshop on in
vivo imaging.
In these
tools, NanoLuc - fluorescent protein fusion shifts NLuc's bright blue luminescence to wavelenghts more friendly for cellular and in
vivo imaging.
Fluorescent proteins are fantastic
imaging tools but their use is limited in
vivo because they must be excited by external light sources.