Sentences with phrase «well cell culture»

The skin biopsies were washed in Ca / Mg - free Dulbecco's Phosphate Buffered Saline (PBS, Invitrogen, Carlsbad, CA, http://www.invitrogen.com) and minced into approximately 12 smaller pieces before being seeded onto gelatin - coated 6 - well cell culture plates (Corning Enterprises, Corning, NY, http://www.corning.com) containing mouse embryonic fibroblast (MEF) media consisting of Dulbecco's Modified Eagle Medium (DMEM) supplemented with 10 % fetal bovine serum (FBS, Invitrogen) and 100 IU / ml penicillin - streptomycin (Invitrogen), and cultured at 37 °C in a 5 % CO2 incubator.

Not exact matches

In my cell biology course, we investigate the biology and chemistry of a cell surface receptor that helps induce good feelings in us when it binds to a chemical compound found in incense; this may help explain why so many different cultures and religions have independently evolved the use of incense in their ceremonies and rituals.

Ohly offers a wide range of products such as yeast extracts, inactive dry yeast, special vitamin yeast, yeast cell wall derivatives, medical yeast and autolysed yeast, based on bakers and / or Torula yeast, as well as wine yeast, specialty powders and starter cultures.

Researchers hope the organoids will be better than lab animals or cells growing in culture at revealing how the human brain develops, both normally and when things go awry, and identify potential therapeutic or genome - editing targets.

The trophoblasts along with blood vessel cells were added to small dextran beads that were then spun around in a container filled with cell culture fluid, creating shear stress and rotational forces to better mimic the environment at the maternal - fetal interface than static cell - culture systems.

With our human gut - on - a-chip, we can not only culture the normal gut microbiome for extended times, but we can also analyze contributions of pathogens, immune cells, and vascular and lymphatic endothelium, as well as model specific diseases to understand complex pathophysiological responses of the intestinal tract.»

«If we win, the entire regulatory structure for autologous cell processing, with or without culture, will be rewritten such that any physician using good practices and treating patients responsibly can use stem cells as part of his or her medical practice,» he says.

Permafrost had preserved tissue from one species — a narrow - leafed campion plant — exceptionally well, so researchers at the Russian Academy of Sciences recently decided to culture the cells to see if they would grow.

Also, researchers could carefully select which blood types to culture with each batch of stem cells, creating stockpiles of needed blood products for people with extremely rare blood types whose blood cell makeup makes it challenging to find good blood matches for transfusions because they would reject most other types of blood.

There will be several rounds of recruitment, and «specifically at this time, we are accepting applications for both junior / senior postdoctoral researchers and recent graduates wishing to enroll in our Ph.D. program as well as personnel with extensive expertise in cell culture and one expert in flow cytometry to fill in the position of manager of the flow - cytometry platform.»

«It's well known in IVF clinics that if you have high glucose concentrations in cell culture, it favours survival of male blastocysts,» says Mathews.

In experiments on cell cultures, both of these inhibitors succeeded in breaking various forms of the TKI resistance: including forms caused by additional mutations of the gene Bcr - Abl as well as those caused by large quantities of the protein Gab2.

«It will be exciting to explore how cell culture conditions enhance the zygote's intrinsic surveillance and repair mechanisms, thus leading to better quality embryos and potentially more successful pregnancies.»

«This small change in injection protocol has resulted in something better than all prior embryo work and cell culture work,» Church says.

To unravel the complex journey of the body's GPCRs, scientists used biophysical tools, including fluorescent biosensors; biochemical tools, such as cell signaling in neuronal cultures; as well as computational models.

In addition to emphasizing the recruitment of graduate students, American universities have a number of on - campus service facilities such as modern research labs, well - stocked reagent rooms, and cell culture facilities.

After the pig experiment worked well, he used his Boston area facility to culture the 30 million cardiac muscle cells that he says were injected into a heart disease patient in mid-2011.

I found that culturing cardiac valve cells on synthetic hydrogels preserves their normal properties better than the traditional plastic plates do, and I discovered a signaling pathway connecting the stiffness of the supporting scaffold to the cells» cytoskeletal structure.

Cultured human cells are a better alternative and are already used by one manufacturer, says the report.

But the transition from brain - cell cultures to the brains of intact animals does not seem to bode well for astronaut resilience.

The three - year study included cell culture studies at Rice as well as a detailed analysis of gene - expression profiles of more than 500 patients from the Cancer Genome Atlas and protein - expression profiles from about 200 MD Anderson patients.

In practice, however, lung cells — especially from older, ill patients — won't grow well enough in culture, but will have to be produced from stem cells or induced pluripotent stem (iPS) cells, she says.

They confirmed low levels of miR - 184 expression in human glioma tissue samples and cultured cell lines as well as an increase in the expression of SND1 compared to normal brain tissue.

Using human T cells in culture as well as mice lacking one of these genes, YY1, they found that, indeed, the two proteins did help move Xist back to the inactive X chromosome in activated lymphocytes.

Using a technique that introduces tiny wrinkles into sheets of graphene, researchers from Brown University have developed new textured surfaces for culturing cells in the lab that better mimic the complex surroundings in which cells grow in the body.

Functional expression of the cDNAs in cultured mammalian cells generated receptors displaying alpha - amino -3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA)- selective binding pharmacology (AMPA = quisqualate greater than glutamate greater than kainate) as well as cation channels gated by glutamate, AMPA, and kainate and blocked by 6,7 - dinitroquinoxaline -2,3-dione (CNQX).

A protein that normally protects cells from environmental stresses has been shown to interact Marburg virus VP24, allowing the deadly Marburg virus to live longer and replicate better, according to a cell culture study led by scientists at the Icahn School of Medicine at Mount Sinai.

«If we can culture the other progenitor cells as well, we'll be closer to building a transplantable kidney.»

Testing many variants, the team eventually found an artificial receptor design that worked well in cell culture, enabling host T cells to efficiently destroy cells producing antibodies to desmoglein, including those derived from PV patients.

They diluted the microbial sample such that they could add a single cell per culture well filled with their new media via a technique called dilution - to - extinction.

To better understand this sensitivity, a cross-disciplinary team of researchers led by Yale created models of the mutation in cell cultures.

Also, in both cell culture and a mouse model of the condition, blocking the excessive splintering reduced production of reactive oxygen species, a potentially harmful byproduct of metabolism, as well as cell death.

Normal cells often have proved impractical because they can only divide a limited number of times in culture, and once returned to the body they're often too old to do much good.

This fact makes the LCSB scientists» results of extraordinary interest to Parkinson's researchers worldwide, as Schwamborn stresses: «On our new cell cultures, we can study the mechanisms that lead to Parkinson's much better than was ever the case before.

Veterinary experts got the healing off to a good start with relentless wound management, a series of smaller skin grafts and the implantation of cell cultures in the wound bed.

Efforts to identify host range and cell tropism of TMAdV, as well as the specific sequence changes responsible for adaptation to growth in cell culture, are currently underway.

Because of these limitations, the AAV approach is best suited for tissue culture based approaches that require modification of only a single allele of a gene, such as the introduction of heterozygous endogenous epitope tags, or the introduction of dominant mutations of a gene into the endogenous allele in cultured cells.

He now juggles two jobs: he is a project manager for the University of Oxford's technology transfer subsidiary ISIS Innovation, as well as managing director of one of its break - out firms, Zyoxel, which develops simple microbioreactors, devices that enable cost - effective 3D cell culture.

Römer and Eierhoff found evidence of the new mechanism in synthetic membranes as well as in cultures of human lung cells.

Pilot studies using a variety of strategies suggested that this goal could be best achieved by stimulating primary organ cell cultures with TNF - α (10 ng / ml) and targeting cells expressing the endothelial cell adhesion molecules E-selectin and VCAM - 1 through a FACS - driven strategy.

Now, researchers at Harvard and Massachusetts General Hospital (MGH) and their colleagues have shown that amyloid - β can protect against yeast and bacterial infections in two animal models, as well as in cultured human cells.

For intracellular cytokine staining, lymphocytes were cultured in 96 - well flat - bottom plates at 1 × 106 cells / well in 200 μl RPMI 1640 supplemented with 10 % FBS in the presence or absence of GP33, GP276, and NP369 peptides.

This process (see figure) started with spin - embryoid body (spin - EB) formation from cultures of single - cell hiPSC suspensions in 96 well plates, and the subsequent addition of BMP4 and FGF - 2 to induce mesoderm differentiation, and then VEGF and SCF to induce hematopoietic differentiation.

The impaired ability of human corneal endothelial cells (HCECs) to divide, both in vivo and in culture, has been well documented in the scientific literature for a number of years [1 - 6].

Besides learning many cell biology techniques (tissue culture, Westerns, generating DNA constructs, transfections) as well as fly genetics, students can get familiar with imaging techniques depending on their own interests and experience.

Overgrown cultures stimulate quiescence, the resting phase of the cell cycle; quiescent cells do not take up nucleic acid or express transgenes as well as actively dividing cells resulting in lower lentiviral titers.

First, cells from Cellartis Human iPS Cell Line 22 (ChiPSC22) were modified to stably express AcGFP1, then these cells were cultured using the DEF ‑ CS culture system in a 48 - well plate.

The present study shows that the basal glucose uptake as well as insulin - stimulated GS activity is reduced in satellite cell cultures established from patients with type 2 diabetes.

The team found that DHEA speeds up the circadian rhythm in human cells, as well as in cultured cells and tissues of mice.

Our lab uses a variety of model systems, such as the nematode C. elegans, as well as in vitro culture systems, to address fundamental questions in cell cycle control during development and tissue formation.