Sentences with phrase «well culture plate»

F1 virgin females were collected, transferred to individual cells of plastic 24 - well culture plates (various manufacturers) and given a single fly host to lay eggs.

The cells were then seeded into 12 - well culture plates (1 ml / well; Falcon, Franklin Lakes, NJ).

Not exact matches

I found that culturing cardiac valve cells on synthetic hydrogels preserves their normal properties better than the traditional plastic plates do, and I discovered a signaling pathway connecting the stiffness of the supporting scaffold to the cells» cytoskeletal structure.

For intracellular cytokine staining, lymphocytes were cultured in 96 - well flat - bottom plates at 1 × 106 cells / well in 200 μl RPMI 1640 supplemented with 10 % FBS in the presence or absence of GP33, GP276, and NP369 peptides.

This process (see figure) started with spin - embryoid body (spin - EB) formation from cultures of single - cell hiPSC suspensions in 96 well plates, and the subsequent addition of BMP4 and FGF - 2 to induce mesoderm differentiation, and then VEGF and SCF to induce hematopoietic differentiation.

First, cells from Cellartis Human iPS Cell Line 22 (ChiPSC22) were modified to stably express AcGFP1, then these cells were cultured using the DEF ‑ CS culture system in a 48 - well plate.

Assays for virus - specific neutralizing antibodies were performed on serial 2-fold diluted samples of each serum using 2 % FBS - DMEM as the diluent in 96 - well tissue culture plates (Falcon 3072); the final volume of the serially diluted samples in each well was 60 µl after addition of 120 TCID50 of SARS - CoV in 60 µl into each well.

For cell proliferation assay, 5 × 104 cells were seeded in triplicate wells per cell line per time point in 12 - well tissue culture plates.

To induce cardiomyocyte differentiation, compact and well - shaped EBs formed in suspension culture containing 10 % of FBS for 6 days were plated in 24 - well plates in the presence of 15 % of FBS.

Briefly, NHLF cells were grown in 96 - well CoStar tissue culture plates (4,000 cells per well) and either subjected to siRNA gene silencing or directly treated with compounds for 51hours in 100 µL complete medium per well.

Sorted human ILC3s (Lin - CD127 +) were cultured at 2,000 — 5,000 cells per well of a 96 well plate with irradiated (30 Gy) OP9 - DL1 feeders [70].

A single - cell suspension (50 μl) containing 1500 cells was mixed with Matrigel (1:1) and plated on top of the Matrigel base onto wells of a 96 - well plate; 50 μl of complete medium was added, and the cells were cultured for 14 days.

EBs were then plated to 12 well culture dishes to allow them to adhere to form a monolayer.

Cells were plated in 12 - well plates at 0.8 × 105 cells / well (A427) or 1.8 × 105 cells / well (H2122) in RPMI medium containing 10 % FBS and cultured overnight.

NHLF cells were cultured in 96 - well Optilux plates as described in Materials and Methods.

EBs were cultured in 6 - well ultra-low attachment plates (Corning, Lowell, MA), and the culture medium was changed every 3 days.

Cells were plated in six - well plates at 2.5 × 105 (A427) or 4 × 105 (H2122) cells / well in RPMI 1640 medium containing 10 % FBS and cultured for 24 h. Culture medium was collected, and PGE2 levels were determined using a PGE2 EIA kit (Cayman Chemicals).

Cells were cultured for 10 d following plating and 12 wells derived from each fraction were subjected to flow cytometry.

(A) BaF3 cells control or stably transduced with wild - type or with V617F, Y931C or V617F / Y931C mutant were washed 3 times in PBS to remove the residual IL - 3 and seeded in 6 - well plates at a concentration of 100,000 cells / mL in culture medium.

These inserts are added to wells containing complete culture medium with 10 % FBS as chemoattractant in 24 - well fluorescence opaque plates allowing only monitoring of fluorescence from the bottom.

Cells were then plated in 24 - well tissue culture treated plates and incubated in a humidified 5 % CO2 incubator at 37 °C.

The skin biopsies were washed in Ca / Mg - free Dulbecco's Phosphate Buffered Saline (PBS, Invitrogen, Carlsbad, CA, http://www.invitrogen.com) and minced into approximately 12 smaller pieces before being seeded onto gelatin - coated 6 - well cell culture plates (Corning Enterprises, Corning, NY, http://www.corning.com) containing mouse embryonic fibroblast (MEF) media consisting of Dulbecco's Modified Eagle Medium (DMEM) supplemented with 10 % fetal bovine serum (FBS, Invitrogen) and 100 IU / ml penicillin - streptomycin (Invitrogen), and cultured at 37 °C in a 5 % CO2 incubator.

Sorted cells cultured in 48 - well plates for 17 days were washed once with PBS and fixed for 5 min.

No differences in the expression of the pluripotent marker Oct4 (Fig. 1C — D) were found between cells cultured in 96 - well plates and hESCs routinely passaged in medium conditioned on MEF in larger dishes (35 - mm or 60 - mm dishes).

People who are prepared to make a break with culinary tradition can reap huge rewards from reconfiguring their plates, and less socially conservative cultures help people to make such a break in tradition, for good or ill.

After starting to attempt to lift itself on day 6, SB - 1 started to slowly crawl on the agar surface of the culture well on day 9, and started to eat the algal food provided the culture plate on day 13.