After 5 minutes of incubation at room temperature in the dark, ViaCount / cell mixture was loaded into a 96 -
well microtiter plate and read by the Guava Flow Cytometer.
Briefly, single - cell suspensions of C57BL / 6 KO and wild - type lymphocytes, which had undergone a 96 hr in vitro boost with γ - irradiated (3,000 rad) BALB / c stimulator lymphocytes, were added to 96 -
well microtiter plates (Corning Inc., Corning, NY) along with 2 × 104 51Cr - labeled BALB / c corneal epithelial or endothelial cells in a total volume of 200 μl / well.
The assays are provided in a pre-coated 96 - or 384 -
well microtiter plates.
The proliferative activity was determined every 24 h by the 3 -(4,5 - dimethylthiazol -2-yl)-2,5-diphenol-tetrazolium bromide assay using a Dynatech MR - 5000 96 -
well microtiter plate reader set at 570 nm.
Not exact matches
Using a standard inverted microscope, researchers can then lift the desired cells and transfer them into individual sample tubes or
microtiter wells.
The Eukaryotic Single - Cell Analysis facility will be placed in Stockholm and will offer single - cell RNA - seq on the Fluidigm C1 platform using the STRT protocol, as
well as in a
microtiter - plate format using SMART - seq2.
On the Persomics ImagineArray ™ platform,
microtiter wells are replaced with contact - printed spots.
These include 5 high performance cell sorters that are customized for isolating cells and chromosomes at high speed and 4 high throughput cell analysers that maximize the speed of acquisition of specimens from
microtiter well plates (96
well plate, 384
well plate).
«The problem is that the
wells on a
microtiter plate have spatial information.