Sentences with phrase «when cre»

cassette upstream of a gene of interest blocks transcription when Cre is absent.

Not exact matches

When tested against a large number of CRE strains, the three compounds were broadly active against the strains.

When using the Gad2 - IRES - Cre knock - in (C57BL / 6J) mouse strain in a publication, please cite the originating article (s) and include JAX stock # 028867 in your Materials and Methods section.

It became possible to design FLEx vectors (Flip - excision vectors) that only allow one inversion event when it was discovered that it is not the exact sequence of the asymmetric target site core but its 8 bp length that is critical for Cre and FLP function.

These differences in recombinase efficiency are important to keep in mind when deciding whether to use the Cre - lox or one of the FLP - FRT systems in your experiments.

When bred to mice expressing Cre recombinase, the STOP sequence is deleted and EYFP expression is observed in the cre - expressing tissue (s) of the double mutant offspriCre recombinase, the STOP sequence is deleted and EYFP expression is observed in the cre - expressing tissue (s) of the double mutant offspricre - expressing tissue (s) of the double mutant offspring.

When bred to mice with a cre recombinase gene under the control of a promoter of interest, the STOP sequence of the targeted gene is deleted in the tissue of interest, and EYFP expression is observed.

al were able to create a split version of Cre that is only active when dimerized by the GBPs / GFP.

were able to create a split version of Cre that is only active when dimerized by the GBPs / GFP.

The accelerated catabolic rate of ApoC - III — depleted TRLs in Ndst1fl / fl Alb - Cre + mice was associated with increased [3H] TRL uptake in the liver (Figure 9D), whereas liver accumulation did not differ when ApoC - III — bearing [3H] TRLs were injected into Ldlr — / — Lrp1fl / fl Alb - Cre + mice (Figure 9F).

In contrast, no difference in the catabolic rate between ApoC - III — depleted and ApoC - III — bearing TRLs was observed when the particles were injected into Ldlr — / — Lrp1fl / fl Alb - Cre + mice (t1 / 2 = 16.2 min vs. 16.8 min, respectively)(Figure 9E).

When combined with a Cre - recombinase - expressing strain, the neomycin cassette and Mir stem loop are removed leaving a lacZ tagged null allele (FRT - lacZ - loxP).

Binding and uptake of radiolabeled ApoC - III — depleted TRLs was significantly increased in hepatocytes isolated from Ndst1fl / fl Alb - Cre + mice (i.e., in mice expressing both LDLR and LRP1) when compared with ApoC - III — bearing TRLs (Figure 10).

When I first heard about the opening of CRES, I managed to visit during a lecture and was very impressed with the hospital.

Radiation Budget of the West African Sahel and its Controls: A Perspective from Observations and Global Climate Models, Miller et al, 8/2012, read more here; ``... GCMs underestimated the surface LW and SW CRF and predicted near zero SW CRE when the measured values were substantially larger...»

The CRE in the 1970s had a series of high - profile battles in the courts when its use of its investigatory powers was challenged by companies and public authorities.

When the HHS Director first approached CRE to bring relationship skills to his employees in Arapahoe County, turnover was occurring at 33 %.

Extra regulatory pressure on CRE lending by smaller banks could hamper commercial property prices at a time when they are already beginning to soften after last year surpassing their pre-crisis peak.

When it's time to list a property, our distribution network reaches the top echelon of all CRE brokers and firms, ensuring that your listing receives maximum exposure.

Let your fellow cre brokers know what to expect when doing a deal with YOU.

«The confusion is not only what goes into the HVCRE bucket on day one, but when those loans can come out of the HVCRE category,» says Christina Zausner, a senior director, policy and industry analysis, for the CRE Finance Council.

In the past when working CRE brokerage exclusively, I would propose this to my buying clients when a competitive buying situation arose, but with the added caviot that my fee went into the deal on a pare pasu equity basis and I also got the management / brokerage work for the project.

Maclin's conservative stance on CRE lending in the mid-2000s helped protect J.P. Morgan Chase from the fallout when the sector crashed in 2008 and 2009.

When first announced, it was feared that risk retention could make CMBS less competitive than other sources of CRE financing.