Sentences with phrase «whole cell lysates»

Lane 1: Wild - type HAP1 whole cell lysate (20 µg) Lane 2: IKB alpha knockout HAP1 whole cell lysate (20 µg) Lane 3: Hela whole cell lysate (20 µg)

Unpurified ab32518 used to immunoprecipitate IKB alpha from human HeLa whole cell lysate.

Unpurified ab32518 used to immunoprecipitate IKB alpha from rat PC12 whole cell lysate.

WB: Jurkat whole cell lysate.

Not exact matches

Whole - cell lysates were prepared 24 and 48 hours following treatment.

Whole - cell lysates were prepared from control and infected cells 48 hours after infection.

Karpas 299 and SU - DHL1 cells were transiently transfected with 20 μg mTOR siRNA and whole - cell lysates were prepared at 48 hours.

(E) Immunoblots were performed on whole - cell lysates (pSTAT5, STAT5, and tubulin) or conditioned medium (MMP1, IL - 6, and TNC) of parental 1833 and SCP28 cells.

(I) Immunoblotting was performed using the indicated antibodies on whole - cell lysates from cells incubated or not with TRAIL.

(K) Immunoblots were performed on whole - cell lysate.

(A to C) Immunoblots with the indicated antibodies were performed on whole - cell lysates of 1833 and SCP28 cells.

(D) Immunoblots were performed on whole - cell lysates (pSTAT5, STAT5, and tubulin) or conditioned medium (MMP1, IL - 6, and TNC).

Western blot analysis of whole - cell lysates demonstrated that ospC7 no longer synthesized OspC (Fig. 3).

Sera from mice obtained 3 weeks after tick - feeding were tested against recombinant B. burgdorferi antigens and whole - cell B. burgdorferi lysates.

Whole - cell lysates from B. burgdorferi and from Escherichia coli expressing recombinant P39 [BmpA (Borrelia membrane protein A)-RSB-(19) or recombinant His - tagged OspC (20) were prepared as described in ref.

The antibody was incubated under agitation with Protein G beads for 10 min, HeLa DFO treated whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10 min under agitation.