Amplicons were visualized in a 2 % agarose gel stained
with Ethidium Bromide and purification was performed directly from the amplification reaction using the Qiagen PCR purification Kit according to the manufacturer's instructions.
PCR products were separated by electrophoresis on a 2 % agarose gel, stained
with ethidium bromide and visualized by UV illumination.
Digested PCR fragments were separated by 10 % PAGE and stained
with ethidium bromide.