Sentences with phrase «with lentiviral vectors»

Kim Y - S, Wielgosz M, Hargrove P, Kepes S, Gray J, Persons D, Nienhuis A. Transduction of Human Primitive Repopulating Hematopoietic Cells with Lentiviral Vectors Pseudotyped with Various Envelope Proteins.

In 2003, VIRxSYS began the first clinical trial with a lentiviral vector.

CD4 + T - cells from patients with HIV - 1 were transduced with a lentiviral vector containing an antisense sequence against the HIV - 1 envelope.

Not exact matches

Single - cell mouse embryos were infected in vitro with recombinant lentiviral vectors to generate transgenic mice carrying the green fluorescent protein (GFP) gene driven by a ubiquitously expressing promoter.

Many times lentiviral vectors are engineered with non-native envelopes, a process called pseudotyping.

But what's more, the transplanted cells keep producing a foreign protein slipped inside with the help of a lentiviral vector, the kind usually used for gene therapy.

A lentiviral vector with the same modifier, Fuw - dCas9 - Tet1CD, is available from Rudolf Jaenisch's lab in plasmid form or as ready - to - use lentivirus.

The library consists of a pool of lentiviral vectors with roughly 73 million semi-random, 30 bp DNA barcodes that are integrated into target cells» genomes upon infection.

Generation of an HIV -1-resistant immune system with CD34 (+) hematopoietic stem cells transduced with a triple - combination anti-HIV lentiviral vector.

Since lentiviral vectors can deliver a large amount of DNA (~ 8 kb) with a relatively low immune response, it should come as no surprise that researchers are interested in developing these vectors for gene therapy.

HEK293T cells were transfected with the following retroviral or lentiviral constructs, with their packaging vectors indicated in the parentheses, using FuGENE 6 reagent (Promega): pMX - puro - STAT5A * (provided by T. Kitamura, University of Tokyo; pCMV - Gag - Pol and pCMV - VSV - G); pLenti - EF - FH - TAZ - S89A (psPAX2 and pVSV - G); PLKO - NS and shTAZ lentiviral construct (provided by C. Linardic, Duke University; pCMV - Rev, pCVM - VSVG; pMDL).

Genes associated with melanoma are overexpressed or silenced with shRNA constructs in lentiviral vectors and the lab increasingly uses cDNA and sh (short hairpin) RNA libraries for our experiments.

Entry vector constructs were recombined into adenoviral (pAd, Invitrogen) or lentiviral (pCSC - Zeo with Gateway recombination sites cloned into the multiple cloning site) destination vectors according to manufacturer's instructions.

Lentivirus was produced by cotransfecting replication incompetent lentiviral vector with psPAX2 (Addgene, 12260) and pLP / VSV - G (Invitrogen).