Sentences with phrase «with agarose»

The improved size resolution of the DNA chip allows students to identify their genotype, something impossible with agarose gel electrophoresis.

Not exact matches

Amplified sequences were visualized by gel electrophoresis in 2 % agarose gels stained with GelRed (Biotium).

A 3 - ml overlay consisting of EMEM with 0.4 % agarose was added, and the cells were incubated at 37 °C for 72 hours.

The bioactivity of agarose — PEGDA interpenetrating network hydrogels with covalently immobilized RGD peptides and physically entrapped aggrecan.

(This is an updated version of the current DNA Fingerprinting field trip, replacing agarose gels with the DNA chip.)

Cell extracts (1.5 mg) were incubated with 15 μL of anti-FLAG M2 - agarose affinity gel (Sigma - Aldrich, St. Louis, MO).

Ten - microliter aliquots of the nested PCR products were subjected to RFLP analysis by digestion with 2 U of either HinfI or MseI, and digested fragments were resolved by agarose gel electrophoresis in TBE buffer, as described elsewhere [19, 28].

Immune complexes were than isolated with E.coli tRNA / Protein A agarose beads, and the obtained purified DNA with subjected to qPCR using primers for HMOX1 E2 promoter.

Immune complexes incubated with protein A agarose slurry containing tRNA for 1 hr at 4 °C with rotation.

PCR products were separated by electrophoresis on a 2 % agarose gel, stained with ethidium bromide and visualized by UV illumination.

Amplicons were visualized in a 2 % agarose gel stained with Ethidium Bromide and purification was performed directly from the amplification reaction using the Qiagen PCR purification Kit according to the manufacturer's instructions.

This activity introduces DNA analysis through DNA digestion with restriction enzymes and agarose gel electrophoresis.

Genomic DNA of clones B31 - A3, ospC7, and ospC7 / ospC +4 was separated through agarose gels, transferred to a membrane, and hybridized with a 32P - labeled probe specific for ospC.

To generate a full - length unc - 9:: gfp construct, the 14.7 - kb UNC - 9A + B PCR product (representing the 5» end of unc - 9) and the unc - 9:: gfp plasmid were digested separately with Kpn I, digests electrophoresed through low - melt agarose (SeaPlaque GTG agarose, FMC, Rockland, ME, USA), and appropriate fragments purified by phenol extraction and ethanol precipitation.

At 24 hours post fertilization (hpf), fluorescent embryos were dechorionated, mounted on coverslips in 1.2 % ultra-low gelling agarose, overlaid with 30 % Danieua / PTU and analyzed by laser scanning confocal microscopy (Zeiss LSM510 Meta).

This product was similarly purified in a low - melt agarose gel and used at 1 ng / μl along with 50 ng / μl of the unc - 36 (+) cosmid derivative RIp16 (gift of L Lobel) for microinjection into unc - 36 -LRB--) animals.

The supernatant was mixed with 300 µl of Ni - NTA agarose (Qiagen), and the proteins were allowed to bind for 3 hours, followed by washing with 20 ml of resuspension buffer, and eluted with 500 µl of elution buffer (20 mM NaHPO4, 300 mM NaCl, 10 % glycerol, 150 mM Imidazole, pH 7.8).

Responsible and highly motivated professional with solid and diverse experience as Molecular Biologist with excellence in isolating and purifying DNA, bacterial plating, polyacrylamide gel electrophoresis, agarose gel electrophoresis.