Sentences with phrase «with cell sorting»

We also thank Robert Karaffa and Sommer Durham for providing essential support with cell sorting and Dr. Ruben Donis (CDC) for his generous gift of ferret anti-H5 serum.

Not exact matches

The close concentration of urban dwellers, combined with plummeting hardware costs and the overlapping layers of wireless data that accompany us — beamed from cell towers, pulsing from Wi - Fi hot spots, radiating from smartphones in our pockets — has made it easier than ever before to track, sort, manage and organize things in a city setting.

If the divine is now used to give the view a supposedly greater philosophical coherence, then I inevitably reach the sort of conclusion implied by Hartshorne's bodily cells with their «little experiences or feelings.»

This depends upon there being a brain, an arrangement of cells in a particular part of the body which by reason of its peculiar coordination makes the given routing able to «know» in a distinctively human manner — quite different from, although certainly continuous with, the sort of «knowing» that is possible for the higher grades of animal life.

We anticipate some sort of growth toward increased complexity: increasingly larger organic macromolecules, then the convergence of many macromolecules to constitute a simple living system, either as a cell with its protective wall and vital nucleus or as some functional analogue, then the convergence of many cells to form larger organisms.

As a physics teacher, I teach the therapeutic effects of gamma radiation in radiotherapy, along with the associated dangers (radiation can cause cells to become cancerous as well as kill cells that are already cancerous), but a common misconception among students is that cancer cells are rather like viruses or bacteria, a sort of alien cell that has entered the body, growing out of control with little relation to the surrounding cells.

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He added: «The important thing is that you work with people who are like - minded and interested in pursuing the same sort of things where you can, and we will, whether the subject is stem cell or whether the subject is climate change.»

Fluorescence - activated sorting is then used to identify and retrieve from this mix only those cells that display strong reporter gene expression, which represent the cells with the most active enhancers.

Eager to sort through the pile of curriculum vitae's on his desk, Rudnicki is particularly looking for molecular geneticists working on different stem cell systems; scientists with genomics bents like proteomics, microarray analysis, and bioinformatics; and a couple of clinician scientists.

In a mix of bacteria that interact with each other to different degrees, the cells sorted themselves according to the mechanical forces they exerted among one another.

They first devised a nanoscale plastic scaffold and then seeded it with common muscle precursor cells called myoblasts, along with endothelial cells of the sort found on the inside of blood vessels.

After sorting out the mutants with the highest activity under washing machine conditions, they put a mixture of the 10 most promising ones into yeast cells.

«Sorting these cells to purity is really difficult and important,» says Darrell Kotton, director of CReM and co-senior author of both papers, with Brian Davis of UTHealth at the University of Texas.

After all, it's much easier to run a computer program than to grow cells, blast them with a compound, and incubate them before getting any sort of results.

On the other hand, the problem is, you know, with embryonic stem cells, they haven't been able to get stem cell lines from livestock animals that can proliferate in that way, without just sort of veering up in their own direction and turning into, instead of muscle, turning into brain tissue or bone tissue or something else.

Using a high - speed actuator as the driving source of pump, they succeeded in producing a flow with 16 microseconds for cell sorting.

«If you can take the patient into the OR, isolate a bunch of their cells, sort them and put them back in that's ideally where we'd like to go with this,» Darling said.

This approach makes it possible to not only classify cells, but also to digitally sort them with a high level of specificity.

«I do think that one day, especially with stem cell advances, we will be able to do this sort of thing routinely — perhaps even with joints or more complex organs.»

A couple of other research groups have also sorted stem cells based on gene expression, but they have not done so specifically with the goal of enriching cell populations for a specific tissue, the researchers said.

«What I find most fascinating is that it suggests that viruses can function with a sort of primitive «hive mentality» to ensure efficient use of host cell resources, akin to the way worker bees tell others where to locate the best food sources.»

In order to identify the cancer stem cells from other cells in the tumor, the research team studied epigenetic mechanisms that distinguish between the least - sorted cells, with endless division properties and a potential to create growth, and the more sorted cells which lack this ability.

The device also prevents the type of damage to cells that can occur with traditional sorting methods, such as those using high - force centrifuges.

«We're sorting individual aggregates full of cells that are active in a simulated environment and with the cell sorter we can grab active cells to study them.

Cells can also be crudely sorted en masse with centrifuges.

«All sorts of reactions in cells are controlled by modifying proteins with kinases and phosphatases,» said Keating.

In order to segregate chromosomes into the two emerging cells, the spindle itself needs a bipolar structure, and so the microtubules must be sorted to align with the long pole - to - pole axis of the spindle.

New research at Rockefeller University, published March 18 in Nature, reveals how cells sort out the loops meant to encode small RNAs, known as microRNAs, by tagging them with a chemical group.

«It's one set of genetically identical cells that are in communication with one another that have a sort of common purpose or at least can coordinate themselves to do something,» Volk explains.

Three men will share the honor for their work describing how small membrane bubbles loaded with molecules get sorted to the correct part of the cell.

«Future sorting techniques based on cell stiffness and existing biomarkers may enable the enrichment of limbal stem cells, thus facilitating clinical success for patients with naturally low limbal stem cell percentages.»

«In combination with recent advances in sorting cells on a biophysical basis, the biomechanical stemness markers we identified hold the potential to rapidly generate corneal transplants with highly enriched stem cell populations, which could one day translate into better outcomes for these patients.»

This disruption halts the maturation of germ cells by interfering with meiosis, the process by which an individual's genetic material is divided and sorted into what eventually becomes eggs and sperm.

The research provides a sort of rulebook that can be used with high success to track down antigens likely to activate a given T cell, easing a bottleneck that has constrained such studies.

Rapsyn is made by our muscle cells and considered a sort of biological anchor that interacts with the acetylcholine receptors to ensure that they are optimally positioned for our muscles to receive orders from our brain.

Flow cytometry and sorting data were analyzed with FlowJo software (Treestar) or Cellquest (BD Biosciences), in the case of primary cells.

We still have our flesh and blood cells, but we soup them up with nanotech brain chips, genetic engineering and these sorts of things.

IDMIT is an infrastructure for preclinical research in infectious diseases and immunology which is certified ISO9001 and which includes 1) A large animal facility with capacity to host NHP in BSL2 and BSL3 containment, 2) State - of - the - art laboratories for cell biology, immunology, molecular biology, flow cytometry and mass cytometry (CyTof), cell - sorting and confocal microscopy in BSL3 containment; 3) A biological resources centre with high storage capacity; 4) Highly innovative technologies for in vivo imaging of large animals in BSL2 and BSL3 containment, including a two - photon microscope, a PET - CT facility, and several optic based technologies (fibered endo - microscopy, near infra - red imaging).

Institutional needs for flow cytometry services were carefully evaluated and a decision was made to transition the operation of the South Campus Flow Cytometry and Cell Sorting Facility to an institutional core to provide the Flow Cytometry and Cellular Imaging Core with sufficient capacity needed to support the institution's investigators.

Whatever the cause, biologists should make sure to note cell sex when describing their experimental methods with stem cells of any sort.

The team has access to state - of - the - art laboratories and animal facilities with BSL2 and BSL3 containment, flow cytometry, cell sorting, histopathology and in vivo imaging (IVIS).

«This is the first time there's been any sort of biochemical activity associated with PMP22,» said Melanie Ohi, Ph.D., associate professor of Cell and Developmental Biology.

BTHP - 1 parental cells were transduced with the MLV vector MX - DC - SIGN and FACS sorted as a population for high levels of DC - SIGN expression.

We delve into very fundamental problems such as «how does a malaria red blood cell attach itself to a blood vessel» or «how does binding of a ligand to a cell surface receptor or contact of a crystalline surface with the plasma membrane drive lipid sorting and how will this lead to signalling» but then immediately apply it to a practical problem such as «how does contact of uric acid crystals with dendritic cells cause gout in affected joints and how can we prevent this occurrence?»

The sorting out of cells with different identities and fates during animal development is an important process to organize functional tissues and organs.

With the proven success of the imaging technique, Larabell said it's possible to perform statistical analyses based on large collections of cell nuclei images sorted by different stages of development.

(C) Sorted Lin - CD127 + cells from Rag1 - / - splenocytes were pretreated with DMSO or indicated concentrations of PD98059 or SB203580 as in A and analyzed for IL - 22 secretion by ELISA.

(A) Human tonsillar lymphocytes or (B) sorted Lin - CD127 + cells were pretreated with lethal toxin (1 μg / ml) for 2 — 3 hr followed by IL - 23 (50 ng / ml) stimulation for 18 hrs.

Sorted human ILC3s (Lin - CD127 +) were cultured at 2,000 — 5,000 cells per well of a 96 well plate with irradiated (30 Gy) OP9 - DL1 feeders [70].